Large Scale Protein Purification Service

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Overview Core Service Offerings Application Areas Project Workflow Case Studies Frequently Asked Questions (FAQs) Contact Us

Overview

CD biosynsis is a professional CRO enterprising who provides large-scale protein purification service. In biotechnology and pharma companies, the protein purification process is an important development step of product safety and quality. We provide the solution of lab to commercial protein purification, applying to the various step of protein purification, from initial expression-isolation to final purification.

Our technical staffs possess experiences to process out protein from different origins, such as antibodies , enzyme and other different therapeutics proteins Due to our advanced chromatography and membrane separation technologies, like ion exchange chromatography, affinity chromatography and ultrafiltration, we can offer the high-purity and high-recovery services at the same time.

Through our large-scale protein purification service, you would have a dependable, efficient and cost-effective partner to fasten the process of drug development and enhance product quality to meet the dynamic market demand.

Monoclonal antibody downstream process(AA Shukla, et al.,2010)

Core Service Offerings

Our company specializes in providing large-scale purification of protein services, which can provide customers with the whole industrial chain of upstream and downstream services from gene synthesis and primary vectors, purified protein, composition analysis and identification. Here is our one-stop service details:

Gene synthesis and optimisation: We use advanced gene design algorithms and codon optimisation to greatly increase the expression level of the target proteins.
Protein expression systems: We provide eukaryotic expression systems and prokaryotic expression systems such as transient gene expression and large-scale protein preparation, stable cell line construction, etc.The largest of our fermenters is 2,000 L and we can comfortably scale the volume of production to any given project.
Purification method of proteins: There have a quite number of proteins purification methods that can be applied for the isolation of proteins, such as affinity column chromatography, gel filtration, ion exchange and hydrophobic chromatography.Such techniques yield proteins of higher than 95 per cent purity.
Label removal and endotoxin removal: We do label removal and endotoxin removal.
Quality Control and Testing. In the process of protein purification,we strictly followed the SOPs and achieve the QC standard, test the target protein and fed back to customer .After purified, personadly go through each of the samples and detect if their antibody reaction is consistent and conforms to customer's needs,so as not to affect customers' use.

Application Areas

Our company specializes in Large scale protein purification services which can be adapted to meet our customer's requirements in pharmaceutical research and development, biopharmaceuticals, science research services and industrial solutions.

Drug research and development: Large-scale protein purification is indispensable in drug research and development. As an instance, large-scale continuous production of drugs for COVID-19 treatment are produced by using large-scale protein purification. Moreover, large-scale protein purification technology is also important in the research and development as well as the production and purification of vaccine for ensuring the virus clearance and removal of host cell proteins in the final product.
Biopharmaceuticals: Biopharmaceutical production often consists of purifying large amounts of protein from cell cultures (recombinant proteins), or from blood collected from donors (antibodies and other biologics), in order to create drugs and other medication with high purity for safety and efficacy.
Scientific research services: Test the functions of enzymes and analyse the 3D structure of a specific protein (example, by X-ray crystallography, NMR spectroscopy etc.). The technique of synthesizing protein used by us involves five steps, the first of which is obtaining the sequence of a target protein. Also, similar large-scale protein purification technology is used to explore the organisation of protein complexes within the cells.
Industrial applications: Ultrafiltration technology to separate proteins on large scale is applied in production line to remove unwanted protein or other ingredients in food processing industry and dairy industry.

Project Workflow

The Large Scale Protein Purification service process provided by our company is as follows:

Large scale protein purification Project Workflow

Material pretreatment and cell disruption: First, the protein is diluted from the tissue or cells and maintained in its natural state without losing its activity.
Rough isolation stage: Separating the target protein from other cellular components (such as RNA, DNA, etc.). Commonly used methods include ammonium sulfate precipitation.
Fine purification stage: Further purify the target protein. Commonly used methods include affinity purification, ion exchange purification and gel filtration purification. These methods separate proteins based on specific properties such as charge, molecular size, etc.
Automated purification steps: Large-scale production utilizes Aktas technology, an automated system designed for efficient, streamlined purification processes.
Sample concentration and clarification: Protein samples are concentrated and clarified using filtration and ultrafiltration techniques.
Dialysis desalination and buffer replacement: Dialysis and washing filtration techniques are used to remove impurities and replace buffer.
Dispensing, lyophilization and labeling: Purified protein is dispensed, lyophilization and labeling based on customer needs (additional time may be required if needed).
Batch production (optional): Large-scale gene expression and purification are carried out according to the quantity required by customers.

Through the above steps, we ensure high-quality, high-purity protein products, consistent from batch to batch and low endotoxin levels.

Case Studies

Customer background: a biotechnology company needs to mass produce recombinant proteins for antibody drug research and development.

Solution: the production of high-purity protein has been successfully achieved by using self built laboratories and a number of independently developed experimental technologies. The experimental success rate is more than 98%. The delivered protein has been successfully applied in antigen production, antibody drug development, diagnostic reagents and vaccine research and other fields.

Results: the recombinant protein with high purity was successfully purified by ion exchange chromatography and gel filtration media, and the purity reached 98%.

Frequently Asked Questions (FAQs)

For the convenience of customers using our large-scale purification of protein services, a list of these common questions and their answers is provided below:

1. What is large-scale protein purification?

The large-scale purification of proteins refers to the ability to isolate active proteins from bioreactors in large amounts using grate equipment and technologies. This expression is frequently used in a bio-pharmaceutical industry which has a requirement of high-purity active proteins to develop drugs and carry out proteinomics research.

2. What technologies are available for large-scale protein purification?

The large-scale protein purification technologies included affinity purification, ion-exchange purification, molecular sieve purification and hydrophobic purification. These technologies had enhanced protein purity and reduced loss of proteins.

3. What equipment is needed for large-scale protein purification?

This process requires the use of fermentation tanks, bioreactors and wave mixers, etc., all of which is used facilitates large-scale protein purification.

4. What is the process for large-scale protein purification?

A large-scale protein purification process involves thepurification of proteins, such as blood cells, plasma, liverprotein, diatoms and phage protein, which includes thefollowing steps: Synthesize genes>Construct expression vectors>Expression protein expression>Protein purificationprotein quality controlEvery step is strictly controlled to ensure the purity and activity of the final product.

5. How high is the purity requirement for large-scale protein purification?

Large-scale protein purification in lab is always challenging, not only for the traditional methods that use bacterial fermenter to express target proteins, but also for transfective methods that cultivate The ultimate goal for protein purification is to achieve very high purity of the target protein, normally over 95%. In order to do that, more advanced AKTA purification systems are available, so is the complete sets of pre-loaded purification columns.

6. What are the applications of large-scale protein purification in drug development?

Large scale protein purification is widely used in the research and development of drugs. It is also important in the production of bispecific antibody drugs, vaccines, and other biological products. The main importance for this lies in the high purity of the active protein.

7. What is the price of large-scale protein purification services?

it depends on the scale of the project and also depends on the selection of instruments Clear price information can be provided if you require our large-scale protein purification service. Please consult your company's sales reps, who will provide you with specific quotations according to your specifications.

Contact Us

We focus on large-scale protein purification services. Our team has rich experience and technology and can provide one-stop services from gene synthesis and vector construction to protein expression and purification to ensure that your project needs are fully met. We use advanced expression systems and large-scale fermentation tanks and other equipment to provide gram-grade protein with a purity of up to 98%. Welcome to contact us to discuss your protein purification needs. Looking forward to cooperating with you!

Please note that all services are for research use only. Not intended for any clinical use.

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