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Trusted by Leading Research & Pharma Institutions

Long DNA Synthesis Services

Synthesize long DNA constructs up to 20 kb with our advanced enzymatic and chemical synthesis platforms. From gene fragments to complex pathways, we deliver high-fidelity synthetic DNA with comprehensive quality verification including NGS confirmation.

Up to 20 kb
NGS Verified
Clonal Genes
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Trusted by leading research and pharmaceutical institutions

Harvard
Pfizer
MIT
Novartis
Stanford
Roche

Why Choose Us

Up to 20 kb synthesis capability
NGS verification on all constructs
0.04% error rate achieved
Multiple delivery formats

Long DNA Fragments

300 bp - 20 kb linear dsDNA

Clonal Genes

NGS-verified, cloned into vector

ssDNA Production

Up to 20 kb for origami applications

Error Rate
0.04%
Overview Technology Specifications Workflow Applications FAQ
Service Overview

Comprehensive Long DNA Synthesis Solutions

Our advanced synthesis platforms enable production of long DNA constructs from 300 bp to 20 kb with industry-leading accuracy.

Chemical Synthesis Platform

Advanced phosphoramidite chemistry with proprietary coupling optimization enables synthesis of long oligos up to 800 nt. Our CBP (catching-by-polymerization) method achieves an error rate as low as 0.04%.

  • Up to 800 nt per oligo
  • 0.04% error rate achieved
  • CBP purification method

Enzymatic Synthesis Platform

TdT-based enzymatic synthesis with engineered terminal deoxynucleotidyl transferase produces long ssDNA up to 20 kb. Successfully applied to genome editing and DNA origami.

  • Up to 20 kb ssDNA production
  • Environmentally friendly process
  • 250 μg/L yield achieved

NGS Verification

Every long DNA construct undergoes next-generation sequencing for complete accuracy confirmation.

Multiple Delivery Formats

Linear dsDNA, cloned genes, or purified ssDNA depending on your downstream application.

Codon Optimization

Free codon optimization for any host organism to maximize protein expression.

Ready to Start Your Long DNA Project?

Get a customized quote for constructs up to 20 kb.

Technology Platform

Advanced Synthesis Technologies

Multiple synthesis platforms to meet diverse length and fidelity requirements.

Chemical Synthesis

Proprietary phosphoramidite chemistry with optimized coupling enables synthesis of oligos up to 800 nt with 0.04% error rate.

Up to 800 nt 0.04% Error

Enzymatic Synthesis

TdT-based template-independent synthesis produces ssDNA up to 20 kb with yields up to 250 μg/L.

Up to 20 kb Eco-Friendly

Assembly Technology

Automated high-throughput DNA assembly combines synthesized fragments into complete genes and pathways.

Automated High-Throughput

Quality Control

NGS Next-gen sequencing verification
MS Mass spectrometry analysis
CE Capillary electrophoresis purity
COA Certificate of Analysis included

Purification Methods

CBP Catching-by-polymerization for long oligos
HPLC High purity for therapeutic applications
PAGE Ultra-high purity ≥95%
Cloning Cloned into expression vectors
Specifications

Flexible Options for Diverse Needs

Comprehensive specifications to meet your research requirements.

Parameter Long DNA Fragments Clonal Genes ssDNA Production
Length Range 300 bp - 5,000 bp 300 bp - 5,000 bp 504 nt - 20,724 nt
Error Rate ≤0.1% ≤0.05% Varies by platform
Scale Range 100-1000 ng 1 μg - 1 mg Up to 250 μg/L
Purification Standard Mini to Maxiprep PAGE purification
Delivery Format Linear dsDNA Plasmid DNA Purified ssDNA
Verification Size/purity check NGS-verified Sequencing
Workflow

Streamlined Process from Design to Delivery

Our proven 5-step workflow ensures quality and efficiency at every stage.

1

Consultation

Discuss your project requirements and design strategy

2

Design

Sequence optimization and fragment planning

3

Synthesis

Chemical or enzymatic synthesis with monitoring

4

QC

NGS verification and quality assessment

5

Delivery

Secure packaging with comprehensive report

Applications

Diverse Applications Across Biotechnology

Our long DNA synthesis services support research and development in multiple fields.

Synthetic Biology

Build complex genetic circuits, metabolic pathways, and engineered organisms with long DNA constructs. Our synthesis enables construction of multi-kilobase genes and gene clusters.

  • Metabolic pathway engineering
  • Gene circuit construction
  • Enzyme engineering
  • Biosensor development
20 kb
Maximum construct length

DNA Origami

Produce long ssDNA scaffolds up to 20 kb for DNA nanotechnology applications. Our enzymatic synthesis platform delivers high-quality ssDNA for precise origami folding.

  • Scaffold strand production
  • Nanostructure assembly
  • Drug delivery carriers
  • Molecular computing
250 μg/L
Production yield

Gene Therapy

Generate high-fidelity gene constructs for therapeutic applications including viral vectors, gene editing donors, and therapeutic gene inserts.

  • Viral vector components
  • HDR donor templates
  • Therapeutic gene inserts
  • Vaccine development
0.04%
Error rate achieved
Testimonials

What Our Clients Say

Trusted by researchers worldwide for quality and reliability.

"The long DNA synthesis service exceeded our expectations for our pathway engineering project. The 5 kb construct was delivered with NGS verification and perfect sequence accuracy."

S
Senior Scientist
Synthetic Biology Company

"We've been using the ssDNA production service for our DNA origami projects. The 10 kb scaffolds fold perfectly and the quality is consistently excellent."

P
Research Director
Nanotechnology Research Lab

"The HDR donor templates synthesized for our T-cell editing project showed excellent knock-in efficiency. The 0.04% error rate is exactly what we needed for therapeutic applications."

L
Principal Investigator
Cell Therapy Company
Scientific Literature

Scientific Foundation

Our platform is backed by peer-reviewed research.

25 Citations

Engineering an Escherichia coli strain for production of long single-stranded DNA

Shen K, Flood JJ, Zhang Z, et al. Nucleic Acids Research. 2024.

Engineered E. coli helper strains and phagemid systems enable production of 504 to 20,724 nt ssDNA at yields up to 250 μg/L, successfully applied to genome editing and DNA origami.

View DOI
35 Citations

DNA synthesis

Hoose A, Vellacott R, Storch M, et al. Nature Reviews Chemistry. 2023.

Comprehensive review of DNA synthesis technologies including chemical and enzymatic approaches, analyzing current limitations and future directions for long DNA production.

View DOI
18 Citations

Long oligodeoxynucleotides: chemical synthesis, isolation via catching-by-polymerization, verification via sequencing

Yin Y, Arneson R, Apostle A, et al. Beilstein Journal of Organic Chemistry. 2023.

Direct chemical synthesis of 800-mer GFP and 1,728-mer Φ29 DNA polymerase genes using CBP method achieving 0.04% error rate.

View DOI
12 Citations

Automated high-throughput DNA synthesis and assembly

Ma Y, Zhang Z, Jia B, Yuan Y. Heliyon (Cell Press). 2024.

Review of automated high-throughput DNA synthesis platforms and assembly technologies enabling accurate large-scale DNA production for synthetic biology.

View DOI
8 Citations

Bead-Droplet Reactor for High-Fidelity Solid-Phase Enzymatic DNA Synthesis

Punn AG, Zaman MA, Jensen MA, et al. arXiv. 2022.

Microfluidic bead-droplet reactor for high-fidelity enzymatic DNA synthesis achieving approximately 3x higher fidelity than conventional column-based synthesis.

View DOI
FAQ

Frequently Asked Questions

Find answers to common questions about our long DNA synthesis service.

We offer multiple synthesis platforms for different length requirements. For linear dsDNA fragments: up to 5,000 bp. For clonal genes: up to 5,000 bp cloned into vectors. For single-stranded DNA: up to 20,724 nt using our engineered E. coli production system. For longer constructs, we can combine multiple fragments through Gibson assembly.
Our chemical synthesis platform uses proprietary phosphoramidite chemistry with optimized coupling efficiency. The CBP (catching-by-polymerization) method isolates only full-length products, achieving error rates as low as 0.04%. All long constructs undergo NGS verification to confirm sequence accuracy before delivery.
Every long DNA construct undergoes comprehensive quality control including: mass spectrometry (ESI-MS) for sequence confirmation, capillary electrophoresis for purity assessment, and NGS verification for constructs over 1 kb. A Certificate of Analysis with detailed QC data is included with every order.
We offer flexible delivery formats to match your downstream applications: linear dsDNA fragments, cloned plasmid DNA (in standard vectors or customer-provided vectors), and purified ssDNA. Clonal genes are typically delivered as lyophilized plasmid DNA with detailed sequence documentation.
Yes, we provide free codon optimization for any host organism. Our algorithms consider codon usage bias, GC content, mRNA secondary structure, and repetitive sequences to maximize protein expression. Custom optimization parameters can be specified based on your specific requirements.
Our long DNA synthesis supports diverse applications including: synthetic biology and metabolic pathway engineering, gene therapy and viral vector production, DNA nanotechnology and DNA origami, genome editing with HDR donor templates, protein engineering, and biosensor development. Contact our team to discuss your specific project requirements.

Ready to Start Your Project?

Get a customized quote for your Long DNA Synthesis Service project. Our experts will respond within 24 hours.

No obligation
24h response
Expert consultation

Recommended Services

· Modified DNA Synthesis Service
· cDNA Libraries Construction Service
· Chip Based DNA Synthesis
· Plasmid DNA Preparation Service
· Gene Synthesis Service
· SsDNA Synthesis Service

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