Mammalian Cells Protein Purification Service

Overview Application Areas Project Workflow Case Studies Frequently Asked Questions (FAQs) Contact Us

Overview

Mammalian cell protein purification is a process to extract and purify protein from mammalian cells.It's often involved in several steps to guarantee a protein is highly pure and retain its biological activity.

To purify proteins from mammalian cells, the protein is first released from the cells. This can be done by either enzymatic or non-enzymatic methods, like using ultrasonic disruption or French press to lyse the cells. The protein is then bound to a matrix to isolate and purify it.

Proteins are purified by techniques such as gel filtration chromatography, ion exchange chromatography, affinity chromatography or hydrophobic chromatography, based on their distinct physical and chemical properties like shape, charge and solubility.

Furthermore, tag purification technology can greatly enhance the efficiency of purification by offering quick, reproducible purification of recombinant proteins produced in mammalian cells. For instance, in some cases, the technique of tandem affinity purification (TAP) can be employed to generate recombinant protein for biochemical research.

Dual-tagging system for the affinity purification of mammalian protein complexes(RJ Giannone, et al.,2007)

Advantages

High Yield and Purity: Optimized protocols to maximize the yield and purity of proteins, ensuring they meet the necessary standards for downstream applications.
Proper Post-Translational Modifications: Mammalian cells ensure that proteins undergo the correct post-translational modifications, such as glycosylation, phosphorylation, and disulfide bond formation.
Scalability: Capable of handling both small-scale lab preparations and large-scale industrial purifications.
Compatibility with Therapeutic Proteins: Particularly suited for the purification of therapeutic proteins, antibodies, and other biologics.
Advanced Purification Techniques: Use of affinity tags, size-exclusion chromatography, ion-exchange chromatography, and other specialized methods to achieve the highest levels of purity and activity.

Application Areas

The mammalian cell protein purification services are used extensively in biomedicine , science and research as well as other fields as the following application scenarios given below:

1. Biomedicine

Drug development: Production of recombinant protein drugs: including antibody drugs, hormones, growth factors, etc.

Vaccine development: production of vaccine antigen proteins.

Drug screening: screening new drug molecules.

Protein structure and function analysis: it is applied to study protein structure and to explain their function mechanism, and provide new ideas for diagnosis and treatment of disease.

Screening of biomarkers: identification of new disease markers of high accuracy accessible for early diagnosis and prognostic evaluation.

2. Biotechnology

Production of enzyme preparation: Industrial enzyme production: enzyme preparations used in food, textile, paper and other industries.

Development of diagnostic reagents: Production of ELISA kits: used to detect markers of various diseases.

Biosensor development: Preparation of proteins required for biosensors.

Protein interaction research: study the interaction between proteins and reveal cell signaling pathways.

Protein modification research: study various modifications of proteins, such as phosphorylation, glycosylation, etc.

Project Workflow

The mammalian cell protein purification service process provided by our company is as follows:

Mammalian protein purification Project Workflow

Obtaining and optimising the gene sequence: Get the gene sequence of the target protein from the customer and optimise the codon to make the mammalian cell expression more effectively.
Gene synthesis and cloning: The optimised gene sequence is synthesised and cloned into a vector for expression in mammalian cells.
Cell transfection and culture : Once you have transfected the mammalian cell using the vector coding the target protein gene, the culturing process can be carried out in large scale in an appropriate culture medium. You may scale up to 1 L culture in lab, alternatively, use the bioreactor cultivation for large scale culture.
Harvesting and extraction: After accumulating enough expression in the cells, the media is harvested, centrifuged and filtered to provide a clear cell lysate.
Preliminary purification: After protein expression was visualised by SDS-PAGE and a green fluorescent band was obtained, preliminary affinity purification was carried out to remove impurities.
Multi-step purification: After extraction, further purification by multi-step purification to obtain the purity with satisfactory yield by applying different purification protocols (affinity chromatography, ion exchange, molecular sieve, etc).
Quality assurance report: We provide protein purification analysis report for customers and our extract, which contains SDS-PAGE, Western Blot and other experimental results to check the purity and activity of the protein.

Through the above steps, we are able to supply customers with different and effective mammalian cell proteins for drug development research, biotechnology and therapeutic development.

Case Studies

Background: In order to provide an experimental basis for early diagnosis reagent bottles and early screening of breast cancer, it is necessary to clone and purify human Mammaglobin protein.
Results: hMAM cDNA was amplified from breast cancer tissue by RT-PCR and expressed in E.coli M15. The fusion protein was successfully purified using Ni-NTA-His affinity chromatography, and the purity reached 97%.

Frequently Asked Questions (FAQs)

For customers who want to know more about mammalian cell protein purification services, we created a list of questions and answers to help them understand better:

1. What is mammalian cell protein purification?

A: Mammalian cell protein purification refers to purification of a specific protein from mammalian cells by specific label into target protein for easy isolate and other unwanted protein.

2.What are the main steps in purifying mammalian cell proteins?

A: steps include: gene design, gene synthesis, cloning, protein production and the generation of stable cell lines that include the M-PER mammalian protein extraction reagent that is used as a specific purification reagent and method.

3.What are the application areas of mammalian cell protein purification?

A: Purification of proteins from mammalian cells is an important tool to aid biomedical reseaching, drug development and biotechnology. By purifying the proteins of mammalian cells recomibant proteins,scientists are able to produce biomolecules that enable more research and help in various kinds of treatments nowadays demonstrated by the wide use of related proteins in medicine nowadays.

4. Which labeling and purification technologies are normally used for protein purification from mammalian cell cultures?

A: Commonly used tags include CMV, EF-1 or UbC promoters.

Contact Us

CD Biosynsis is dedicated to serving your needs in mammalian cell protein purification. We are a team of professional scientists working with state-of-the-art purification technologies and customized solutions to help to proceed your project. Whether you require research-level service or industrial-scale one, precise and effective purification process and services make sure that your project go smoothly. We could constantly overcome your challenge to contribute to your research in biomedicine and biotechnology. If you have any inquiries, please contact us for more information.

Please note that all services are for research use only. Not intended for any clinical use.

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