Escherichia coli Metabolic Engineering

Introduction

Escherichia coli (E. coli) is a versatile and widely used microorganism that has been extensively studied and engineered for various biotechnological applications. Metabolic engineering of E. coli involves the manipulation of its metabolic pathways to produce valuable compounds, such as biofuels, chemicals, and pharmaceuticals, as well as to improve its growth and survival in different environments.

At CD Biosynsis, we have extensive experience in metabolic engineering of E. coli, and we are committed to providing high-quality services and solutions to our clients.

Figure 1: The primary lutein biosynthetic pathway. (Park, S.Y., et al. 2022)

Our Services for Metabolic Engineering of E coli

CD Biosynsis offers a range of services for metabolic engineering of E. coli, including:

Metabolic Pathway Engineering

We design and engineer E. coli metabolic pathways to produce a wide range of compounds, such as:

• Biofuels (e.g., butanol, ethanol, and isobutanol)
• Chemicals (e.g., biodegradable plastics, detergents, and fragrances)
• Pharmaceuticals (e.g., antibiotics, anti-inflammatory agents, and vaccines)

Gene Editing and Gene Expression

We use state-of-the-art gene editing tools, such as CRISPR-Cas9, to modify genes involved in metabolic pathways and to improve gene expression.

Fermentation Optimization

We optimize E. coli fermentation conditions to improve cell growth and productivity, including the selection of optimal media compositions, pH, temperature, and oxygen levels.

Process Development and Scale-Up

We develop and scale up optimized fermentation processes to meet clients' specific needs, including the design and construction of bioreactors and the development of process control strategies.

Pilot Plant and Commercial Scale Production

We offer pilot plant and commercial scale production services for clients who require large quantities of engineered E. coli strains.

Our Advantages

At CD Biosynsis, we have a team of experienced scientists and engineers who are dedicated to developing innovative solutions for metabolic engineering of E. coli. Our advantages include:

• Deep understanding of E. coli metabolism and its genetic manipulation.
• State-of-the-art biotechnology platforms and tools.
• Proven track record of successfully engineered E. coli strains.
• Collaborative and customized approach to meet clients' specific needs.

Metabolic engineering of E. coli is a powerful tool for synthetic biology, allowing for the production of a wide range of valuable compounds. At CD Biosynsis, we are committed to providing high-quality services and solutions to our clients, leveraging our expertise and cutting-edge technologies. Whether you are looking to produce biofuels, chemicals, or pharmaceuticals, we have the capabilities and experience to help you succeed. Contact us today to learn more about our services and how we can help you unlock the full potential of synthetic biology.

References

1. Park, S.Y., et al. Metabolic engineering of Escherichia coli with electron channelling for the production of natural products. Nature Catalysis, 5, 726–737 (2022).
2. Zhao, X.R., et al. Metabolic engineering of Escherichia coli for secretory production of free haem. Nature Catalysis, 1, 720–728 (2018).

Please note that all services are for research use only. Not intended for any clinical use.