Strain Metabolic Profiling

Metabolic Profiling Services for Biological Research

Metabolic profiling, also known as metabolomics, is the large-scale study of metabolites present in cells, tissues or biofluids. Metabolites are small molecules such as amino acids, sugars, and lipids that are the end products of cellular processes. By measuring changes in metabolites, researchers can gain a snapshot of an organism's biochemical state and how it responds to stimulus. Metabolic profiling is thus a powerful tool for characterizing biological systems, identifying disease biomarkers, and studying drug effects and toxicities.

Figure 1: Hierarchical Cluster Analysis (HCA) based on LC-MS metabolic profiles from the organic extracts of 24 bacterial strains and TSB culture media. (Betancur Luz A., et al. 2017)

CD Biosynsis provides comprehensive metabolic profiling services to support biological research and drug development. Metabolic profiling analyzes the small molecules or metabolites in a biological sample, providing insight into cellular function and phenotypic changes.

Our Metabolic Profiling Services

Targeted Metabolite Analysis

We offer targeted metabolic profiling using LC-MS, GC-MS and CE-MS techniques to quantify hundreds of predefined metabolites covering key biochemical pathways.

• Amino Acids: All 20 proteinogenic amino acids plus many non-protein amino acids
• Organic Acids: TCA cycle intermediates, ketone bodies
• Sugar Phosphates: Glycolysis and pentose phosphate pathway intermediates
• Lipids: Glycerolipids, glycerophospholipids, sphingolipids, sterols
• Nucleotides and Cofactors

Sample Preparation and Analysis Workflow

• Samples are extracted using optimized organic solvent mixtures to maximize metabolite recovery
• Extracts are subjected to liquid-liquid or solid phase extraction for purification and concentration
• Targeted metabolites are separated using high-resolution chromatography
• Metabolites are ionized and detected by high-sensitivity mass spectrometry
• Data is processed using proprietary pipelines for peak identification, integration and QC
• Statistical analysis is performed to detect differential metabolites

Custom Service Offerings

When standard panels do not meet your needs, we provide fully customized methods development and validation.

• Custom metabolite panels
• Strain-specific extraction and analysis methods
• Specialized sample types (tissues, biofluids, microbes)
• Validation to regulatory standards.

Benefits of Our Approachs

• High Sensitivity: Our experienced scientists utilize optimized sample preparation and analytical methods to achieve the highest sensitivity and reproducibility.
• Wide Coverage: Our metabolic profiling panels cover over 500 metabolites spanning primary and secondary metabolism. Customized methods are also available.
• Robust Data Analysis: We employ rigorous QC, data normalization and advanced statistical analysis to generate biologically meaningful datasets. Validation is performed on every project.
• Experience: With years of experience, CD Biosynsis has supported hundreds of metabolic profiling studies for applications such as strain engineering, biomarker discovery and toxicology assessment.

At CD Biosynsis, we aim to provide the highest quality metabolic profiling data and insights to help advance your research and development goals. Our experienced scientists and customized multi-platform approaches allow us to map unexpected metabolic changes and uncover new biological mechanisms. Contact us today to learn more about our services and we look forward to helping you achieve your next breakthrough.

References

1. Lin Y.X., et al. Comparative metabolomics shows the metabolic profiles fluctuate in multi-drug resistant Escherichia coli strains. Journal of Proteomics, 2019, 207: 103468.
2. Dempo, Y., et al. Molar-Based Targeted Metabolic Profiling of Cyanobacterial Strains with Potential for Biological Production. Metabolites, 2014, 4, 499-516.
3. Betancur Luz A., et al. Marine Actinobacteria as a source of compounds for phytopathogen control: An integrative metabolic-profiling / bioactivity and taxonomical approach. PLOS ONE, 2017, 12(2): e0170148.

Please note that all services are for research use only. Not intended for any clinical use.