Metabolite Analysis

CD Biosynsis provides a wide range of metabolite analysis services for the test phase of synthetic biology to help researchers around the world facilitate their cutting-edge research. Equipment with an advanced technology platform and powerful synthetic biology tools, we have the ability to help bring diverse synthetic biology applications and creative ideas to fruition.

Introduction to Metabolite Analysis

One of the primary goals of synthetic biology is to generate desired materials and reduce production costs through a good conversion from substrates to products. This goal can be achieved through metabolic engineering, which enables the optimization of genetic and regulatory processes and pathways within cells to increase the production of a whole range of valuable substances while reducing the energy costs to the cells and the production of any unwanted side products. Metabolomics is one of the indispensable tools for synthetic biology to quantify global small biomolecules. The combination of synthetic biology and metabolomics is an evolving field.

The key to advancing metabolic engineering beyond trial-and-error research is to obtain components with well-defined performance that can be readily applied in vastly different environments with predictable effects. Currently, analytical tools that allow the detection of target molecules, transcripts, proteins, and metabolites from different hosts and pathways are critical in this field. In-depth omics analysis can provide a system-level view on the specific information of thousands of strain variants generated by screening technologies. These analyses can provide quantitative information on the dynamic processes between components and host chassis, thus contributing to better decision-making during the design and strain construction stages to accelerate metabolic engineering research. Metabolic engineering and synthetic biology, two promising areas of biotechnology, have made great contributions to the resolution of problems in the production of chemical compounds, drugs, vaccines, etc.

Figure 1. The design–build–test–learn cycle of metabolic engineering highlighting important parts of each of the components. (Petzold C J, et al., 2015)

Featured Services

CD Biosynsis offers a complete set of tools for metabolite analysis and synthetic biology to help our customers overcome the challenges in synthetic biology. Browse our featured services below or use our online inquiry form to get more information.

Polar and Non-polar Metabolite Analysis

Polar metabolites, including nucleic acids, amino acids, sugars, and small organic acids, are typically part of primary metabolism. And often play a role in growth and development. Non-polar metabolites often encompass secondary metabolites with non-polar or hydrophobic chemical properties, which can provide evolutionary advantages important for survival. CD BioSicences provides both polar and non-polar metabolite analysis services to fully meet customer needs.

Metabolomics

Metabolomics aims to systematically identify and quantify the metabolome of biological systems at specific time points. It plays an increasingly central role in the Design–Build–Test–Learn (DBTL) cycle of synthetic biology. CD Biosynsis has established a comprehensive metabolomics platform that includes, but is not limited to targeted and untargeted metabolomics to advance metabolic engineering and synthetic biology.

Fluxomics

Fluxomics aims to determine the rates of metabolic reactions within a biological entity. The integration of fluxomics within synthetic biology is crucial at all levels from the initial design to the optimization. CD Biosynsis has developed a fluxomics platform to provide a more detailed analysis of metabolic fluxes.

CD Biosynsis offers a variety of cutting-edge techniques to customize services for individual customer specifications. Our experienced scientific team of biologists, bioinformatics, and chemists is dedicated to helpinging our customers solve any problems encountered in their synthetic biology projects.

If you are interested in our services or have any specific needs, please feel free to contact us for more details.

References

1. Petzold C J, et al. Analytics for metabolic engineering. Frontiers in bioengineering and biotechnology, 2015, 3: 135.
2. Nguyen Q T, et al. Metabolomics methods for the synthetic biology of secondary metabolism. FEBS letters, 2012, 586(15): 2177-2183.

Please note that all services are for research use only. Not intended for any clinical use.