MicroRNA Agomir/Antagomir Synthesis

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MicroRNA Agomir/Antagomir Synthesis is an advanced and innovative technology that enables the synthesis of specific microRNA molecules, known as Agomir or Antagomir. These molecules play a vital role in the regulation of genes and have significant implications in various biological processes. By manipulating the expression of microRNAs, researchers can gain valuable insights into the underlying mechanisms of diseases and potentially develop new therapeutic strategies.

Service Process Case Studies FAQs

Service Process

Our service process is designed to provide you with a seamless and efficient experience. Here is an overview of the steps involved:

Consultation: We believe in the importance of understanding your research requirements thoroughly. During the consultation phase, our team will engage in detailed discussions with you to gain insights into your research objectives, experimental techniques, and specific needs. Based on this understanding, we will provide expert guidance on the design and synthesis of Agomir/Antagomir molecules.
Design and Synthesis: Our team of experienced scientists will leverage their expertise in microRNA synthesis to design and synthesize Agomir/Antagomir molecules that are tailored to your research objectives. We will consider the specific characteristics of the molecules, such as sequence, modifications, and delivery methods, to optimize their effectiveness.
Quality Control: Ensuring the highest quality and purity of the synthesized molecules is of utmost importance to us. Each synthesized Agomir/Antagomir molecule undergoes rigorous quality control measures, including analytical techniques and functional assays, to verify its purity and integrity. We employ advanced technologies and follow strict protocols to guarantee the reliability and reproducibility of the results.
Delivery: Once the quality control process is complete, we will promptly and securely deliver the synthesized Agomir/Antagomir molecules to your specified location. We understand the sensitivity of these molecules and take extra precautions to ensure their stability and integrity during transit.

We are committed to providing you with the highest quality MicroRNA Agomir/Antagomir Synthesis services. Our team of experts is dedicated to supporting your research objectives and ensuring that you have the necessary tools to make significant advancements in your field. Contact us today to learn more about how we can assist you in your research journey.

Case Studies

Elucidating the regulatory mechanisms of osteogenesis of human mesenchymal stem cell (hMSC) is important for the development of cell therapies for bone loss and regeneration. Here we showed that hsa-miR-199a-5p modulated osteogenic differentiation of hMSCs at both early and late stages through HIF1a pathway. hsa-miR-199a expression was up-regulated during osteogenesis for both of two mature forms, miR-199a-5p and -3p. Over-expression of miR-199a-5p but not -3p enhanced differentiation of hMSCs in vitro, whereas inhibition of miR-199a-5p reduced the expression of osteoblast-specific genes, alkaline phosphatase (ALP) activity, and mineralization. Furthermore, over-expression of miR-199a enhanced ectopic bone formation in vivo. Chitosan nanoparticles were used for delivery of stable modified hsa-miR-199a-5p (agomir) both in vitro and in vivo, as a proof-of-concept for stable agomir delivery on bone regeneration. The hsa-mir199a-5p agomir were mixed with Chitosan nanoparticles to form nanoparticle/hsa-mir199a-5p agomir plasmid (nanoparticle/agomir) complexes, and nanoparticle/ agomir complexes could improve the in vivo regeneration of bone. Further mechanism studies revealed that hypoxia enhanced osteogenesis at early stage and inhibited osteogenesis maturation at late stage through HIF1a-Twist1 pathway. At early stage of differentiation, hypoxia induced HIF1a-Twist1 pathway to enhance osteogenesis by up-regulating miR-199a-5p, while at late stage of differentiation, miR-199a5p enhanced osteogenesis maturation by inhibiting HIF1a-Twist1 pathway.

Preparation and characterization of nanoparticle/agomir. (A) hsa-mir-199a-5p agomir was first complexed with the TPP. Next, the agomir:TPP complexes were added to an chitosan solution, spherical particles of submicron size encapsulating agomir were generated as shown by the scanning electron micrograph. (B) the SEM of the nanoparticles/ agomir. (C) in vitro Toxicity the nanoparticles/agomir. Viability of human MSCs incubation with the nanoparticles/agomir extract. (D) The nanoparticle size distribution calculated from electron micrograph images shows the mean diameters of nanoparticles/agomir ranging from 0.1 to 0.8 um. Scale bar ¼ 10 um, 1um.

(X Chen, et al.,2015)

FAQs

Here are some frequently asked questions about our MicroRNA Agomir/Antagomir Synthesis services:

Q: What is the turnaround time for Agomir/Antagomir synthesis?

A: The turnaround time for Agomir/Antagomir synthesis depends on various factors, including the complexity and quantity of the molecules. During the initial consultation, our team will assess your specific requirements and provide you with an estimated timeline.

Q: Can you assist with the design of Agomir/Antagomir molecules?

A: Absolutely! Our team of experts is well-versed in the design and optimization of Agomir/Antagomir molecules. We will work closely with you to understand your research objectives and provide guidance on designing molecules that are tailored to your specific needs.

Q: Are your Agomir/Antagomir molecules compatible with different experimental techniques?

A: Yes, our synthesized Agomir/Antagomir molecules are designed to be compatible with a wide range of experimental techniques. We understand the diverse nature of research projects and ensure that our molecules can be seamlessly integrated into various experimental setups, allowing you to explore different avenues in your research.

Q: Do you offer bulk synthesis services?

A: Yes, we understand that some research projects require larger quantities of Agomir/Antagomir molecules. We offer bulk synthesis services to accommodate such needs. Please contact us for more information and pricing details, and we will be happy to assist you further.

Q: What are the delivery options for the synthesized Agomir/Antagomir molecules?

A: We offer flexible delivery options to accommodate your specific needs. Depending on your location and requirements, we can arrange for shipping via express courier or provide electronic delivery of the synthesized molecules. Our team will work closely with you to determine the most suitable delivery method.

Q: Can you provide technical support after the delivery of the synthesized Agomir/Antagomir molecules?

A: Yes, we offer comprehensive technical support to ensure your success in using the synthesized molecules. Our team of experts is available to answer any questions, provide troubleshooting assistance, and offer guidance on the application of the molecules in your research. We are committed to supporting you throughout your research journey.

Q: Are your MicroRNA Agomir/Antagomir Synthesis services customizable?

A: Absolutely! We understand that each research project has unique requirements. Our services are highly customizable to meet your specific needs. Whether you require modifications to the molecule design, specific analytical assays, or additional quality control measures, we can tailor our services to align with your research objectives.

Q: Do you provide documentation and certificates of analysis for the synthesized Agomir/Antagomir molecules?

A: Yes, we provide detailed documentation and certificates of analysis for the synthesized Agomir/Antagomir molecules. These documents include information on the synthesis process, purity assessment, quality control results, and specifications of the molecules. We ensure that you have comprehensive records to support your research and regulatory requirements.

Q: How can I request a quote for MicroRNA Agomir/Antagomir Synthesis services?

A: To request a quote, please reach out to our team via email or phone. Provide us with details about your research project, including the specific Agomir/Antagomir molecules you require, quantities, any customization needs, and desired delivery options. Our team will promptly review your request and provide you with a detailed quote.

Please note that all services are for research use only. Not intended for any clinical use.

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