NAD+/NADH Colorimetric Assay Kit

Catalog Number: AKAS0438

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Specifications

Product Information
Product Name	NAD+/NADH Colorimetric Assay Kit
Detection Method	Colorimetric method
Instruments	Microplate Reader
Sample Type	Animal Tissue, Cell
Detection Principle	Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.
Assay Time	70 min
Other Reagents	PBS(0.01 M, pH 7.4)
Storage	-20°C

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