Strain Engineering for Nucleocapsid Protein

CD Biosynsis has successfully established a variety of gene expression systems and is committed to promoting research related to viral structural proteins using in vitro expression systems to express a variety of viral nucleocapsid proteins.

Implications for Synthetic Biology-Driven Nucleocapsid Protein Production

The nucleocapsid protein is the main protein of the viral capsid, which has nucleoprotein properties, binds to nucleic acids, protects them, and plays an important role in the process of virus proliferation and infection. Currently, there are various methods to study the function of viral proteins, among which, in vitro expression of viral structural proteins followed by functional studies is one of the most common means. Based on synthetic biology techniques, viral recombinant structural proteins are obtained by prokaryotic or eukaryotic expression systems and can be used for activity analysis, screening and characterization of viral mutants, and study of virus-host cell interaction mechanisms. Therefore, as the main structural protein of viruses, the large-scale production of nucleocapsid proteins through synthetic biology is of great importance for the study of many viruses.

Figure 1. Viral nucleocapsid proteins (NCs) function in several stages of the virus life cycle. (Ding B, et al., 2016)

What We Provide

Based on the synthetic biology platform, our scientists are dedicated to exploring the application of genetic engineering, protein engineering, and other technologies in the process of nucleocapsid protein production.

Deliverables

• Nucleocapsid protein products.

How We Can Help

Production of Nucleocapsid Protein

We have successfully established a variety of gene expression systems for the production of nucleocapsid proteins. Depending on the expression host, they can be classified as E. coli expression systems, yeast expression systems, mammalian expression systems, and plant expression systems. Based on the above in vitro expression systems, we have realized the production of various viral nucleocapsid proteins. If you would like to synthesize nucleocapsid proteins of other viruses by synthetic biology methods, please contact us directly.

Optimization of In Vitro Expression of Nucleocapsid Protein

Nucleocapsid proteins are expressed at low levels in vitro, are weakly immunogenic, and do not produce an effective, durable, and widespread cytosolic humoral immune response in the secondary organism. We help our customers to optimize the in vitro expression of nucleocapsid proteins through the following services.

• Selection of suitable expression vectors and host systems. We are able to help our customers select the appropriate expression vector and host system based on the structural characteristics of the nucleocapsid protein and the research objectives.
• Optimization of codons for genes encoding nucleocapsid proteins. Common codons exist for each genetically engineered organism. In order to enable high levels of expression of viral genes in the host expression system, we can help our customers to make the coding sequence complexes of viral genes express the host-preferred codon usage.
• Improve translation termination efficiency. The vast majority of organisms have their own favored planting codons in addition to codon preferences that encode proteins. We are able to help our customers optimize sequences near termination codons to significantly increase the expression of recombinant proteins.

Applications of Nucleocapsid Protein

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive nucleocapsid protein production and meet the needs of customers in a variety of industries.

• As a target antigen for vaccine development.
• For the development of specific monoclonal antibodies.
• Used for disease diagnosis.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in nucleocapsid protein production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Ding B, et al. Nucleocapsid proteins: roles beyond viral RNA packaging. Wiley Interdiscip Rev RNA. 2016 Mar-Apr; 7(2): 213-26.

Please note that all services are for research use only. Not intended for any clinical use.