Nucleotide Strain Engineering

CD Biosynsis is dedicated to harnessing the power of synthetic biology to produce custom nucleotides efficiently and reliably. By meticulously recreating the intricate nucleotide biosynthesis pathway, we offer a comprehensive range of nucleotide synthesis services, ensuring access to high-quality building blocks crucial for various life sciences applications.

Background

Nucleotides serve as the fundamental building blocks of life, playing an indispensable role in the biochemistry of living organisms. These organic molecules are the structural units of nucleic acids, namely DNA (deoxyribonucleic acid) and RNA (ribonucleic acid), which are pivotal in storing and transmitting genetic information. Nucleotides consist of three key components: a phosphate group, a pentose sugar (either ribose in RNA or deoxyribose in DNA), and a nitrogenous base. These complex molecules act as the alphabet of life, encoding genetic instructions and facilitating various cellular processes, making them central to the field of molecular biology and genetics.

Fig 1. Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylation. (Chen L, et al., 2023)

The biosynthesis of nucleotides, a highly regulated and intricate cellular process, is vital for maintaining the integrity and functionality of DNA and RNA. This biosynthetic pathway ensures a constant supply of nucleotides required for DNA replication, repair, and RNA transcription.

Nucleotide Biosynthesis Pathway

The de novo synthesis of nucleotides primarily occurs through two distinct pathways: the purine and pyrimidine biosynthesis pathways. Here are the key stages of the nucleotide biosynthesis pathway:

Services Offering

Our synthetic biology technology allows us to biosynthesize a wide range of nucleotides, including:

• Purine Nucleotides. Purine nucleotides are the building blocks of DNA and RNA, which are essential for the replication and transcription of genetic material.
• Pyrimidine Nucleotides. Pyrimidine nucleotides, such as cytosine (C) and thymine (T), are vital for DNA synthesis. In RNA, uracil (U) replaces thymine. Pyrimidine nucleotides are also involved in various metabolic pathways.
• Deoxyribonucleotides. Our technology enables the synthesis of deoxyribonucleotides critical for DNA replication and repair.
• Ribonucleotides. Our capabilities encompass the synthesis of ribonucleotides necessary for various cellular processes, including protein synthesis and gene regulation.
• Modified Nucleotides. Beyond the basic nucleotides, we also specialize in the synthesis of modified nucleotides. These modifications can include methylations, acetylations, and other chemical alterations that influence gene expression and cellular function.

Applicationsof Biosynthesis Nucleotide

• Drug Development: Essential for antiviral and anticancer drug research.
• Genomic Studies: Essential for DNA sequencing, polymerase chain reaction (PCR), and next-generation sequencing (NGS).
• Nutraceuticals: Essential for the formulation of dietary supplements and nutraceutical products.
• Biotechnology: Employed in gene editing (e.g., CRISPR-Cas9), synthetic biology, and recombinant protein expression.
• Diagnostic Tools: Used in various diagnostic assays for diseases, genetic disorders, and infections.

Our Technical Advantages

Whether you require custom nucleotide synthesis for cutting-edge research or large-scale production for pharmaceutical applications, we have the expertise and technology to meet your needs. Explore the endless possibilities of biosynthesized nucleotides and unlock new horizons in biology and medicine. Feel free to contact us.

Reference

1. Chen, L.; et al. Direct stimulation of de novo nucleotide synthesis by O-GlcNAcylation. Nature Chemical Biology. 2023, 1-11.

Please note that all services are for research use only. Not intended for any clinical use.