Oligo Synthesis is the process of creating short sequences of nucleic acids, known as oligonucleotides. These oligonucleotides are widely used in various research applications, such as PCR, gene synthesis, and DNA sequencing. They play a crucial role in molecular biology and biotechnology.
Oligo Synthesis(P Herdewijn, et al.,2008)
Below is a table outlining the application areas and requirements for oligo synthesis:
Application Area | Description | Requirements |
---|---|---|
PCR and DNA Sequencing | Primer design for PCR amplification and DNA sequencing. | Knowledge of target sequence, primer design software, DNA synthesizer, suitable reagents (nucleotides, phosphoramidites). |
Gene Synthesis | Construction of synthetic genes for various purposes. | Accurate sequence design, DNA synthesizer, high-quality reagents, purification methods, sequence verification. |
Site-Directed Mutagenesis | Introduction of specific mutations into DNA sequences. | Design of mutagenic primers, DNA synthesizer, reagents for PCR, sequence verification methods. |
Probe Synthesis | Production of labeled probes for molecular detection. | Design of probe sequence, labeled nucleotides or post-synthesis labeling, purification methods, quality control. |
Aptamer Synthesis | Generation of specific DNA or RNA molecules for binding to target molecules. | Rational design of aptamer sequence, DNA or RNA synthesizer, purification methods, binding assays. |
RNA Interference (RNAi) | Synthesis of short interfering RNA (siRNA) for gene silencing. | Design of siRNA sequences, DNA or RNA synthesizer, purification methods, validation of gene knockdown. |
Functional Genomics | Synthesis of oligos for various functional genomics studies. | Design of oligo sequences, DNA synthesizer, purification methods, validation assays. |
Our Oligo Synthesis service process is designed to be seamless and efficient, ensuring a hassle-free experience for our customers. Here's how it works:
If you have any further questions or require additional information, please don't hesitate to contact our customer support team. We are committed to providing exceptional service and support for all your Oligo Synthesis needs.
Here are some frequently asked questions about our Oligo Synthesis services:
A: Our standard turnaround time is [insert time frame], but expedited options are available upon request. We understand that some projects have urgent timelines, and we offer flexible solutions to meet your specific needs.
A: Absolutely! We offer various modification options to meet your specific research needs. Whether you require specific chemical modifications, labeling, or other customizations, our team of experts is here to assist you. Please consult our customer support team for further assistance, and they will guide you through the available options.
A: Quality is our top priority. We employ stringent quality control measures throughout the entire synthesis process. Our experienced technicians utilize advanced analytical techniques such as High-Performance Liquid Chromatography (HPLC) and mass spectrometry to ensure the purity and accuracy of the synthesized oligos. Rest assured that our quality control procedures are in place to deliver reliable and consistent results for your research.
A: Yes, we understand that large-scale projects may require cost-effective solutions. We offer competitive pricing and tailored solutions for bulk orders. Please reach out to our customer support team to discuss your requirements, and they will provide you with the best pricing options.
A: We value transparency and communication with our customers. Once your order is placed, you will receive regular updates regarding the status of your synthesis. You will be notified at various stages, including confirmation of shipment. Additionally, our customer support team is always available to address any queries or concerns you may have regarding your order.
A: We accept various payment methods, including credit cards, bank transfers, and online payment platforms. Our customer support team will provide you with detailed payment instructions and assist you throughout the payment process.
A: Absolutely! We have a team of experienced scientists and technical experts who can provide guidance and support for your experiments. Whether you need assistance with troubleshooting or optimizing experimental conditions, we are here to help. Please reach out to our customer support team with your specific requirements, and they will connect you with the appropriate experts.
A: Yes, we offer sample purification options to ensure the highest quality oligonucleotides for your research. Our purification methods include cartridge-based purification, HPLC purification, and PAGE purification. Please consult our customer support team for more information and guidance on choosing the right purification method for your specific needs.
A: Absolutely! We provide a certificate of analysis (COA) with every synthesized oligo order. The COA includes detailed information on the quality control tests performed, including HPLC data, mass spectrometry analysis, and purity results. You can rely on our COA to validate the quality and specifications of your oligonucleotides.
A: Our synthesis capabilities allow for a wide range of oligo lengths and complexities. However, there may be practical limitations depending on the specific sequence and modifications requested. Our team of experts will work closely with you to ensure that your desired oligo can be successfully synthesized. Please consult our customer support team with your specific sequence requirements, and they will provide guidance based on our capabilities.
Please note that all services are for research use only. Not intended for any clinical use.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
There is no product in your cart. |
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.