Orthogonal Genetic Systems in Strains

In recent years, the field of synthetic biology has made significant advancements in developing new genetic systems that allow for precise and efficient control of gene expression and editing. One promising approach is the use of orthogonal genetic systems, which enable the simultaneous regulation and editing of multiple genes with high specificity and efficiency. CD Biosynsis is leading the way in providing cutting-edge services in this exciting orthogonal genetic systems field.

Figure 1: The abstract formation of an orthogonal DNA replication system by Plasmid/DNA polymerase pair. (Arzumanyan G.A., et al. 2018)

Our Services for Genetic Circuits Design

Our services in orthogonal genetic systems offer a comprehensive solution for designing, engineering, and testing custom genetic circuits for gene regulation and editing. At CD Biosynsis, we use advanced computational tools and state-of-the-art technologies to design and engineer custom circuits that are tailored to meet the specific needs of our clients. Our services include:

Circuit Design and Engineering

We work closely with our clients to design and engineer custom genetic circuits that meet their specific needs. Our team of experts uses advanced computational tools to model and predict the behavior of these circuits, allowing us to optimize their performance and efficiency.

Our circuit design and engineering services include the following:

• Design of custom genetic circuits based on client specifications.
• Optimization of circuit performance through computational modeling and simulation.
• Selection of genetic components and assembly of circuits using standard molecular biology techniques.
• Modification of existing circuits to meet new specifications.

We use a modular approach to circuit design, which enables us to easily swap in and out different genetic components to create new circuits with different functions. This approach allows us to rapidly prototype and test different circuit designs, reducing the time and cost involved in developing new genetic systems.

Testing and Validation

Once a custom genetic circuit has been designed and engineered, we perform rigorous testing and validation to ensure that it meets the desired specifications. We use a variety of techniques, including quantitative PCR, fluorescence microscopy, and high-throughput sequencing, to analyze the behavior of the circuit in vivo.

Our testing and validation services include the following:

• Testing circuit behavior in vivo using advanced molecular biology techniques.
• Quantitative analysis of circuit performance using qPCR and other assays.
• Microscopic imaging to visualize circuit behavior in vivo.
• High-throughput sequencing to analyze large datasets.

We also perform extensive validation studies to ensure that the circuit is highly specific and efficient, and that it does not interfere with the host cell's natural regulatory networks. This allows us to ensure the safety and efficacy of the system before it is used in downstream applications.

Applications

Our services in orthogonal genetic systems have a wide range of applications in various fields, including biotechnology, agriculture, and medicine. One promising application is in the development of new gene therapies for the treatment of genetic diseases.

Our application services include the following:

• Development of new gene therapies for the treatment of genetic diseases.
• Engineering of crops that are resistant to pests and disease.
• Design of custom circuits for use in biofuels research.
• Development of new diagnostic tools for the detection of disease.

Our Advantages

• Experienced Team
• Customized Solutions
• Cutting-Edge Tools and Technologies
• Wide Range of Applications

In CD Biosynsis, our services in orthogonal genetic systems offer a number of advantages, including an experienced team, customized solutions, cutting-edge tools and technologies, and a wide range of applications. Contact us today to learn more about how we can help you with your next project in orthogonal genetic systems.

References

1. Arzumanyan G.A., et al. Mutually Orthogonal DNA Replication Systems In Vivo. ACS Synthetic Biology, 2018, 7(7): 1722-1729.
2. Zhang Yang, et al. Development of Host-Orthogonal Genetic Systems for Synthetic Biology. Advanced Biology, 2021, 5(3): 2000252.

Please note that all services are for research use only. Not intended for any clinical use.