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Oxaloacetate Colorimetric Assay Kit

Catalog Number: AKAS0464

Price: Online Inquiry

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Specifications

Product Information
Product Name Oxaloacetate Colorimetric Assay Kit
Detection Method Colorimetric method
Instruments Microplate Reader
Sample Type Animal Tissue
Detection Principle Oxaloacetic acid (OAA), one of the intermediates in the tricarboxylic acid cycle is an important substance in carbon and nitrogen metabolism. Oxaloacetate reacts with substrate acetyl-coA under the action of enzyme. The substance produced can react with DTNB, which has the maximum absorption peak at 412 nm. The content of oxaloacetate can be determined by measuring the absorbance value.
Assay Time 15 min
Other Reagents Incubator, Ultrafiltration tube (50kD)
Storage -20°C

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