Strain Phenotypic Screening and Selection

Introduction

Phenotypic screening and selection is a crucial step in the development of novel strains with improved performance in synthetic biology. With the increasing demand for high-value products and sustainable solutions, there is a growing need for efficient and effective screening methods that can identify the most promising strains for further development. At CD Biosynsis, we offer a range of services for phenotypic screening and selection, utilizing cutting-edge technologies and expertise in synthetic biology to deliver the best possible results for our clients.

Figure 1: General workflow for selection and high-resolution phenotypic screening of bacterial mutants in the nwSlide. (Antypas H., Veses-Garcia M., et al. 2018)

Our Services for Phenotypic Screening and Selection

High-throughput Screening

High-throughput screening is a powerful tool for rapidly assessing the performance of large numbers of strains. At CD Biosynsis, we utilize a variety of high-throughput screening technologies, including DNA microarrays, RNAi, and CRISPR/Cas9. These technologies allow us to target specific genes and pathways and identify the most effective combinations of genetic traits.

Mutagenesis and Phenotypic Selection

Mutagenesis and phenotypic selection is another powerful tool for generating novel strains with improved performance in specific environments or applications. At CD Biosynsis, we employ a mutagenesis and phenotypic selection approach that involves generating a collection of random mutants, followed by phenotype analysis to identify strains with desirable traits. This approach has been successful in a range of applications, including the domestication of Chlamydomonas reinhardtii strains for improved performance in photobioreactors.

Natural Product-Based Screening

Natural product-based screening is a valuable tool for identifying novel compounds and drug candidates with promising biological activity. At CD Biosynsis, we utilize natural product-based screening to identify compounds and drug candidates with potential applications in synthetic biology and beyond.

Our Advantages

At CD Biosynsis, we pride ourselves on our expertise in synthetic biology and our cutting-edge technologies for phenotypic screening and selection. Our advantages include:

1. High-Quality Data: We utilize state-of-the-art equipment and protocols to ensure high-quality data and reproducible results. Our rigorous quality control measures ensure that our clients can rely on our data for their decision-making processes.
2. Customization: We work closely with our clients to develop customized solutions that meet their specific needs and goals. Our team of experts has the knowledge and experience to design and execute experiments that deliver the best possible results for each project.
3. Rapid Turnaround Time: We understand the importance of timely results in the fast-paced world of synthetic biology. As a result of our high-throughput screening methods and efficient workflows, we are able to deliver results quickly and efficiently at a minimum turnaround time of 6 weeks, while maintaining the highest degree of quality and accuracy.

Conclusion

Phenotypic screening and selection is a crucial step in the development of novel strains with improved performance in synthetic biology. At CD Biosynsis, we offer a range of services for phenotypic screening and selection, utilizing cutting-edge technologies and expertise in synthetic biology to deliver the best possible results for our clients. With our customized solutions, high-quality data, and rapid turnaround time, we are committed to helping our clients achieve their goals in the field of synthetic biology. Contact us today to learn more about our services and how we can help you with your phenotypic screening and selection.

References

1. Antypas H., Veses-Garcia M., et al. A universal platform for selection and high-resolution phenotypic screening of bacterial mutants using the nanowell slide. Lab on a Chip, 2018, 18(12): 1767-1777.
2. Thewes S., Moran G. P., et al. Phenotypic screening, transcriptional profiling, and comparative genomic analysis of an invasive and non-invasive strain of Candida albicans. BMC Microbiology, 2008, 8(1): 187.
3. Cairns T., Sidhu Y., et al. Construction and high-throughput phenotypic screening of Zymoseptoria tritici over-expression strains. Fungal genetics and biology, 2015, 79:110-7. doi: 10.1016/j.fgb.2015.04.013.

Please note that all services are for research use only. Not intended for any clinical use.