Porcine Chemokine Array (12 targets)

Catalog Number: AKAA093

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Specifications

Product Information
Product Name	Porcine Chemokine Array (12 targets)
Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Analytes	CCL3L1 Eotaxin-1 (CCL11) IFN alpha IFN-beta IFN-gamma IL-1 alpha (IL-1 F1) IL-1 beta (IL-1 F2) MIF MIG (CXCL9) MIP-1 beta (CCL4) RANTES (CCL5) TNF alpha
ASSAY SIZE	2 Arrays/4 Arrays/8 Arrays/
Product Features	Easy to useNo specialized equipment neededCompatible with nearly any liquid sampleProven technologyHighly sensitive (pg/ml)Sandwich ELISA specificityHigher density than ELISA, Western blot or bead-based multiplex
Kit Components	Porcine Chemokine Antibody Array C1 MembranesBlocking BufferWash Buffer 1Wash Buffer 2Biotinylated Detection Antibody CocktailStreptavidin-Conjugated HRPDetection Buffer CDetection Buffer DLysis Buffer8-Well Incubation TrayPlastic SheetsArray TemplatesOther Materials RequiredPipettors, pipet tips and other common lab consumablesOrbital shaker or oscillating rockerTissue Paper, blotting paper or chromatography paperAdhesive tape or Saran WrapDistilled or de-ionized waterA chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	1.Block membranes2.Incubate with Sample3.Incubate with Biotinylated Detection Antibody Cocktail4.Incubate with HRP-Conjugated Streptavidin5.Incubate with Detection Buffers6.Image with chemiluminescent imaging system7.Perform densitometry and analysis
Species	Porcine
Number of Targets Detected	12
Gene Symbols	CCL4, CCL5, CXCL9, IFN-BETA, IFNB, IFNB1, IFNG, IL1A, IL1B, INTERFERON-BETA-1\|INTERFERON-BETA/POIFN-BETA, MIF, MIP1B, SCY4A, SCYA5, TNF, TNFA, TNFSF2
Compatible Sample Types	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Storage	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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