Porcine Inflammation Array (26 targets)

Catalog Number: AKAA098

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Specifications

Product Information
Product Name	Porcine Inflammation Array (26 targets)
Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Analytes	Eotaxin-1 (CCL11) GM-CSF IFN alpha IFN-beta IFN-gamma IL-1 alpha (IL-1 F1) IL-1 beta (IL-1 F2) IL-1 Ra (IL-1 F3) IL-10 IL-12 p70 IL-13 IL-15 IL-17A IL-17F IL-18 IL-21 IL-22 IL-28B IL-4 IL-6 IL-8 (CXCL8) MIF MIG (CXCL9) MIP-1 beta (CCL4) RANTES (CCL5) TNF alpha
ASSAY SIZE	2 Arrays/4 Arrays/8 Arrays/
Product Features	Easy to useNo specialized equipment neededCompatible with nearly any liquid sampleProven technologyHighly sensitive (pg/ml)Sandwich ELISA specificityHigher density than ELISA, Western blot or bead-based multiplex
Kit Components	Porcine Inflammation Antibody Array C1 MembranesBlocking BufferWash Buffer 1Wash Buffer 2Biotinylated Detection Antibody CocktailStreptavidin-Conjugated HRPDetection Buffer CDetection Buffer DLysis Buffer8-Well Incubation TrayPlastic SheetsArray TemplatesOther Materials RequiredPipettors, pipet tips and other common lab consumablesOrbital shaker or oscillating rockerTissue Paper, blotting paper or chromatography paperAdhesive tape or Saran WrapDistilled or de-ionized waterA chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	1.Block membranes2.Incubate with Sample3.Incubate with Biotinylated Detection Antibody Cocktail4.Incubate with HRP-Conjugated Streptavidin5.Incubate with Detection Buffers6.Image with chemiluminescent imaging system7.Perform densitometry and analysis
Species	Porcine
Number of Targets Detected	26
Gene Symbols	CCL4, CCL5, CSF2, CXCL8, CXCL9, IFN-BETA, IFNB, IFNB1, IFNG, IGIF\|IL10, IL12A, IL13, IL15, IL17, IL17A, IL17F, IL18, IL1A, IL1B, IL1RN, IL21, IL22, IL28B, IL4, IL6, IL8, INTERFERON-BETA-1, INTERFERON-BETA/POIFN-BETA, IRAP1, MIF, MIP1B, SCY4A, SCYA5, TNF, TNFA, TNFSF2
Compatible Sample Types	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Storage	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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