Strain Promoter Engineering

Introduction

Promoter engineering is an important tool in synthetic biology that involves modifying the DNA sequences that control gene expression to optimize the production of specific proteins or metabolites. This approach has become increasingly popular in recent years as it allows for fine-tuning gene expression levels to achieve desirable outcomes. One of the main applications of promoter engineering is in the development of strains for biotechnology. CD Biosynsis is a leading company in the field of synthetic biology, providing expert services in promoter engineering for strains.

Figure 1: Overview of promoter engineering methods. (John B., Hal S.A. 2013)

Our Services for Promoter Engineering

CD Biosynsis offers a comprehensive range of services for promoter engineering in strains, including promoter screening, characterization, substitution, and strain optimization. Our team of experts has extensive knowledge and experience in the field of synthetic biology and uses updated technologies to deliver high-quality results to our clients.

Promoter screening is the first step in our promoter engineering service. We use RNA-seq and promoter prediction to identify endogenous strong promoters in the target strain. This approach has the advantage of utilizing the natural regulatory elements of the host organism, which can increase the efficiency and stability of gene expression.

After identifying potential promoters, we characterize their strength using reporter gene assays. This allows us to evaluate the transcriptional levels of genes under the control of different promoters and select the most suitable promoter for the target gene.

Once the optimal promoter has been identified, we substitute it upstream of the target gene to increase transcriptional levels. This can significantly increase the production of desired metabolites or proteins in the target strain.

In addition to promoter engineering, we also offer strain optimization services. This involves combining promoter engineering with other strategies such as gene knockout or overexpression to further optimize the production of desired metabolites or proteins.

Our Advantages

• Extensive Experience

With years of experience in the industry, CD Biosynsis has a proven track record of success in promoter engineering and strain development.

• State-of-the-Art Equipment

Our laboratory is equipped with the latest tools and equipment.

• Customizable Services

We offer highly customizable services that can be tailored to meet the specific needs of our clients.

Promoter engineering is a powerful tool in synthetic biology that can significantly increase the production of desired metabolites or proteins in strains. CD Biosynsis is a leading company in the field of synthetic biology, offering a comprehensive range of services for promoter engineering in strains. Our team of experts has extensive knowledge and experience in the latest technologies and techniques in synthetic biology, and we utilize state-of-the-art technologies to deliver high-quality results to our clients. Contact us today to learn more about how we can help you achieve your goals in promoter engineering and strain development.

References

1. John B., Hal S.A. Promoter engineering: recent advances in controlling transcription at the most fundamental level. Biotechnology journal, 2013, 8(1): 46-58.
2. Li-Qun, J., Wei-Rong, J. et al. Promoter engineering strategies for the overproduction of valuable metabolites in microbes. Applied microbiology and biotechnology, 2019, 103(21-22): 8725-8736.
3. Xu, N., Wei, L. et al. Recent advances in the applications of promoter engineering for the optimization of metabolite biosynthesis. World Journal of Microbiology and Biotechnology, 2019, 35(33).

Please note that all services are for research use only. Not intended for any clinical use.