Protein Analysis Services

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Overview Core Service Offerings Technology Platform and Advantages Application Areas Project Workflow Case Studies Frequently Asked Questions (FAQs) Contact Us

Overview

Protein Analysis Services refers to a series of experimental technologies and services used to characterize proteins. These services cover everything from the detection, purification, and identification of proteins to detailed analysis of their structure and function. These services are widely used in fields such as biomedical research, drug development and industrial production.

Representative LC-MS/MS data and a generalized bioinformatic analysis pipeline for protein identification and quantification in shotgun proteomic(Y Zhang, et al.,2013)

Core Service Offerings

The Protein Analysis Services services provided by our company include the following:

Protein expression profiling: By studying changes in protein expression in specific cells or tissues under specific states, it helps researchers discover potential biomarkers and understand the role of proteins in the disease process.
Protein-protein interaction network analysis: Build an interaction network between proteins to study the interactions between proteins and their biological functions.
Mass spectrometry for protein measurement: including protein sample preparation, enzymatic hydrolysis, mass spectrometry analysis, database retrieval and protein identification. Mass spectrometry can accurately detect the relative molecular mass, amino acid sequence and post-translational modifications of proteins and polypeptides in biological samples.
Quantitative protein analysis: Determine protein concentration using BCA, Bradford and other methods, and measure protein concentration by ultraviolet spectrophotometer.
Protein purity analysis: High performance liquid chromatography (HPLC) technology is used for protein purity analysis, and proteins of different sizes, shapes, or hydrophilic and hydrophobic properties are separated through chromatographic columns.
Proteomic analysis: Including total protein profiling and post-translational modification (PTM) type analysis, such as identification and quantification of modification sites such as phosphorylation, glycosylation, and acetylation.
Protein functional analysis: Study the function of proteins and their role in cell biological mechanisms through methods such as GO classification and metabolic pathway enrichment analysis.
Construction of differential protein interaction networks: Construction of interaction networks based on differential proteins is used to study the interactions of proteins under specific conditions and their biological significance.
Transcription factor prediction: Predicting the likelihood of a protein serving as a transcription factor and its role in the regulation of gene expression.

These services cover the entire process from protein extraction, quantification, identification to functional analysis, providing researchers with comprehensive protein analysis solutions.

We offer a comprehensive suite of Protein Analysis Services tailored to meet the needs of researchers and professionals in the fields of pharmaceutical research, biotechnology, and academia. Our services cover a broad spectrum of analytical techniques designed to assess the structure, function, purity, and stability of proteins. With state-of-the-art instrumentation and a team of experienced scientists, we provide accurate and detailed protein analysis to support your research, development, and production projects.

Technology Platform and Advantages

The Protein Analysis Services services provided by our company mainly rely on multiple technology platforms. These platforms include but are not limited to:

LC-MS/MS technology: This technology is used for proteomic data analysis to support comprehensive analysis of non-targeted and targeted data.
Bioinformatics software: For example, PEAKS Online uses the PEAKS 12 software workflow to provide accurate and sensitive proteomic analysis.
Glycosylation analysis: Some platforms also provide glycosylation analysis services to study glycosylation modifications of proteins.
Protein interaction analysis: Including protein-protein and protein-small molecule interaction analysis.

These technology platforms together form the core technical foundation of Protein Analysis Services provided by our company, helping customers conduct protein analysis and verification during the drug development process.

Application Areas

The Protein Analysis Services services provided by our company are mainly used in the following fields:

Cell or tissue proteomic analysis: This includes proteomic analysis with and without treatment, which helps study the mechanism of action and toxicity of drugs.
Discovery and verification of biomarkers: Proteomic technology plays an important role in the discovery and verification of biomarkers, which is of great significance for early diagnosis and treatment of diseases.
Research on drug mechanism of action and toxicity: Through proteomic analysis, we can deeply study the mechanism of action and potential toxicity of drugs, thereby optimizing drug design and development.
Disease mechanism research: Proteomics plays a key role in studying the mechanisms of various diseases, such as cancer, helping to understand the pathological process of diseases.
Target identification and verification: Proteomic technology can help identify and verify new drug targets, which is crucial for new drug development.
Clinical diagnosis and drug screening: Proteomics provides theoretical basis and technical support for clinical diagnosis and drug screening, helping to improve diagnostic accuracy and drug screening efficiency.
Toxicology testing and antibody analysis: Proteomic techniques also have important applications in toxicology testing and antibody analysis, helping to assess the safety and effectiveness of drugs.

To sum up, the Protein Analysis Services services provided by our company are widely used in many fields such as pharmaceutical research and development, disease research, and clinical diagnosis, and can provide customers with comprehensive proteomic analysis solutions.

Project Workflow

The main process of providing Protein Analysis Services can be divided into the following steps:

Contract signing: First of all, after receiving customer needs, the company will communicate technical solutions with customers in a timely manner. After determining the technical service plan, complete the quotation, communicate the price and service details with the customer, and finally sign the contract.
Project implementation: After signing the contract, the company drafts the project document according to the customer's project plan, and delivers the project document to the corresponding experimental personnel to start project implementation.
Sample preparation: For protein analysis services, you first need to prepare samples. Samples can come from a variety of sources without special requirements. Freshly collected samples need to be directly frozen and stored in a refrigerator at-80℃.
Protein detection and identification: Use a variety of experimental techniques for protein detection, purification and identification. These techniques include but are not limited to SDS-PAGE electrophoresis, mass spectrometry, liquid chromatography, etc. For example, Coomassie-stained SDS-PAGE and LabChip capillary electrophoresis are used to analyze molecular weight and examine molecular expression and secretion.
Protein structure and functional characterization: Through high-resolution mass spectrometry detection and professional software analysis of data, the primary structural sequence of the protein is determined to ensure 100% sequence detection coverage. In addition, secondary, tertiary and quaternary structure prediction and functional characterization of proteins can also be carried out.
Data analysis and reporting: Conduct detailed analysis of experimental data and generate a comprehensive analysis report. The report includes information on the identity, purity, quantity and quality of the protein. In addition, it may also include differential quantification, differential metabolite screening, and advanced machine learning analysis.
Project conclusion: After the project is completed, submit a project conclusion report to explain the project completion and provide all relevant experimental data and analysis result documents.

Through the above processes, the company is able to provide customers with high-quality protein analysis services to support various needs in research, production and clinical development.

Case Studies

Here are a few case studies on protein analysis services:

Label free non-standard quantitative technology was used to compare the differences in protein abundance between leaf age and nitrogen nutrition. A total of 2717 proteins were identified in all treatments and were divided into 4 comparison groups, namely "LM vs LS", etc. This study helped customers deeply understand the differences in protein expression under different conditions and provided important data support for subsequent biological research.
Proteomic testing was carried out on Mycoplasma pneumoniae C267 and M129 strains through TMT-labeled quantitative proteomic analysis, and a total of 7263 polypeptides corresponding to 531 proteins were detected. This study revealed differentially expressed proteins between macrolide-sensitive and drug-resistant strains, providing important data support for the development of new antibiotics.

Frequently Asked Questions (FAQs)

As a CRO company that provides protein analysis services, customer communication and business promotion are very important. The following common customer questions:

1. What are the basic principles of protein mass spectrometry identification?

The basic principle of protein mass spectrometry identification is to digest the protein into a mixture of peptide fragments with a protease, ionize it by soft ionization means (such as MALDI or ESI), and then separate the peptide fragments with a specific plaster-to-core ratio through a mass analyzer. The analysis was carried out through the actual spectrum and theoretically the primary mass spectrum peak and secondary mass spectrum peak generated by protein digestion.

2. What is the difference between IP-MS and Co-IP MS?

IP-MS (Immunoprecipitation Mass Spectrometry) is mainly used to detect specific proteins and their interactions, while Co-IP MS (Co-Immunoprecipitation Mass Spectrometry) is used to study interactions between proteins. The main difference between the two lies in the different experimental design and application purposes.

3. In statistical analysis of differentially expressed proteins, how do you know what the most significant protein is?

In the statistical analysis of differentially expressed proteins, the most significant differentially expressed protein can be determined by calculating the change in expression amount of each protein and combining statistical methods (such as t-test or ANOVA). In addition, visualization tools such as volcano maps can be used to help identify significantly different proteins.

4. Can WB films be used for mass spectrometry?

Western Blot (WB) membranes are usually not directly used for mass spectrometry analysis. Mass spectrometry requires protein samples, not protein bands separated by electrophoresis. Therefore, the proteins on the WB membrane need to be properly processed and extracted before they can be used for mass spectrometry analysis.

5. What is the role of enzymatic hydrolysis in peptidomics research?

Enzymolysis is one of the key steps in peptidomics research. It refers to the use of specific enzymes to break down polypeptide chains, so that originally large and complex proteins or polypeptides are broken down into smaller and simpler polypeptides or amino acids. This facilitates subsequent mass spectrometry analysis.

6. How are sample controls used in data analysis?

Sample controls are pooled plasma samples that are run repeatedly on each experimental plate and are used as external controls to estimate the internal and external precision of each assay. These control samples help correct experimental data and ensure the reliability of the results.

7. What is the difference between intact protein analysis and peptide-based proteomics methods?

Complete protein analysis can provide fingerprints of samples and reveal differences in proteins between samples, such as post-translational modifications (PTMs). Compared with peptide-based proteomic methods, complete protein analysis highlights differences between protein isomers and reduces sample complexity and sample pretreatment requirements.

8. What are the classic and accurate methods for protein analysis?

Classic and accurate methods for protein analysis include proteomic analysis methods such as protein molecular weight determination, mass spectrometry identification, and iTRAQ (TMT). These methods can provide detailed information on proteins, including their structure, function and interactions.

9. How to select the right CRO company to provide protein analysis services?

When selecting a suitable CRO company, factors such as the company's qualifications and successful experience, priority scheduling, and the best project team should be considered. In addition, we should also ensure that CRO companies have abundant resources and complete equipment.

10. What is the role of proteomic analysis services in drug development?

Proteomic analysis services can help early discovery teams identify and verify drug targets during development, reveal key biomarkers for the pharmacology team, and determine mid-target and off-target effects and mechanisms of action. This is of great significance for drug development.

Contact Us

Our team of experts is proficient in a variety of mass spectrometry techniques and strategies and can provide high-quality characterization information for your protein and sugar chain structures. In addition, we also provide computational protein analysis services that use principles of bioinformatics, molecular modeling and systems biology to extract meaningful information from biological data.

If you have any needs or questions about protein analysis, please feel free to contact us. We look forward to working with you to jointly promote the progress of scientific research.

Please note that all services are for research use only. Not intended for any clinical use.

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