Protein Expression in Yeast

Overview Capabilities Advantages Services Process FAQs

Overview

CD Biosynsis is dedicated to delivering excellent yeast protein expression services. The yeast expression platform is an effective eukaryotic expression platform for large-scale production of recombinant proteins. This system possesses the best features of prokaryotic and eukaryotic expression systems and can undergo post-translational changes (glucosylation, methylation, etc.) returning foreign proteins to their native form.

Our yeast expression solutions provide the entire service spectrum, ranging from gene cloning and plasmid building to protein expression and purification. We employ stable production strains and optimize protein yield and purity by utilizing signal peptides, promoters and induction systems. Additionally, our yeast expression system also features low cost and high efficiency, making it particularly suitable for recombinant proteins that require mass production.

Our services include protein expression and purification on a small to large scale, for a range of downstream uses, including antibody production, drug development and biomarker creation. We can now, using advanced gene editing tools and metabolic engineering methods, continue to improve the expression level and function of proteins.

Yeast expression vectors and selective markers(R Baghban, et al.,2019)

Capabilities

The following are the one-stop service details of yeast protein expression services

Number	Step	Description	Turnaround	Deliverables
1	Gene synthesis and codon optimization (optional)	Gene synthesis is carried out according to the sequence provided by the customer, and codon optimization is carried out to improve expression efficiency.	1-2 weeks	sequencing report
2	vector construction	The optimized genes were cloned into appropriate expression vectors and subjected to plasmid sequencing.	1-2 weeks	Cloning into expression vector, plasmid sequencing, large-scale plasmid preparation
3	Vector linearization and transformation	The vector is linearized and transformed into the appropriate yeast strain.	1 week	vector linearization, transformation of the linearized vector into appropriate strains, PCR identification of positive transformants, screening of high-copy transformants
4	expression and purification	Protein expression is carried out in yeast and preliminary purification is carried out.	1-2 weeks	Feasibility report, CoA
5	Expression evaluation and optimization	The expressed protein was subjected to solubility analysis, SDS-PAGE analysis, etc., and expression conditions were optimized if necessary.	1-3 weeks	Expression assessment report, plasmid sequencing, host transformation, positive strain screening, SDS-PAGE analysis, protein purification (soluble expression), QC analysis
6	QC analysis	The final protein samples were tested for purity, concentration, charge heterogeneity, etc.	3 days	SDS-PAGE and UV analysis, HPLC-SEC purity analysis and endotoxin determination (optional)

The exact time may vary based on the complexity of the project and customer needs.

Advantages

The primary advantages of yeast expression systems in protein expression go something like this:

Expression at a high level

The yeast expression system has the ability to express foreign proteins at a high level (in Pichia pastoris it's 10-100x more than Saccharomyces cerevisiae).

Post-translational modifications

Yeast can do many post-translational modifications like glycosylation, disulfide bonding, and phosphorylation to leave the protein as it is.

Cost-effectiveness

Yeast systems are less expensive than mammalian cell systems and their culture conditions are very simple which makes them ideal for industrial scale.

Rapid growth

Yeast Cells Propagates Very Fast and has the capability to grow rapidly up to a cell density within a short period of time and is therefore suitable for production of large scale fermentation.

Easy to operate

The yeast expression system is straightforward in its genetic history, easy to use, and simple in genetic engineering and improvement.

High safety

Proteins generated by yeast expression systems tend to be non-toxic, and many yeast strains are GRAS (Generally Recognized As Safe) certified and biopharmaceutical safe.

Suitable for large-scale production

The yeast expression system can handle industrial production at large scales and can generate recombinant proteins reliably and reliably.

Services Process

The following is the cooperation process for yeast protein expression services:

Early engagement

In the beginning, the client messages or calls the company to discuss needs.

Confirmation of requests and service plan development

The organization issues a detailed service plan in response to customer's requests and contacts customers for confirmation.

Contract signing

When the two parties reach a consensus about service content, price, and other terms, a contract is signed.

Project implementation

Vector design: copy the target gene sequence into an expression vector.
Create a strain: Choose an appropriate yeast strain, like Saccharomyces cerevisiae or Pichia pastoris.
Small scale expression analysis: Run small scale yeast expression experiments to measure protein expression.
Correction and tweaking: Based on the initial experimental findings, expression conditions were modified and high-expression clones were chosen for further study.
Expression at a mass scale: 1-liter yeast was expressed using shake flasks and proteins were purified.

Project delivery

Demo sub-packaging & warehousing: Demo sub-packaging & warehousing for storage.
Writing a report: Prepare a project report or a composition review report.
Product delivery: Plan product delivery and deliver reports as per the needs of the customer.

After-sales services

Provide technical assistance and the additional services required to ensure customers can smoothly operate the delivered protein products.

Early engagement

Confirmation of requests and service plan development

Contract signing

Project implementation

Project delivery

After-sales services

The above procedure is intended to provide customers with end-to-end solutions from gene cloning to protein expression and purification for high-quality yeast protein expression services.

FAQs

Q: Why should we use yeast protein expression services?

A: Yeast protein expression system has the best of both worlds: prokaryotic and eukaryotic expression systems, high expression, mature fermentation, low cost, simple isolation and simple post-translational modification. Further, the yeast expression system can withstand the undesirable conditions of mass continuous fermentation, increasing yields.

Q: How long the cycle of yeast protein expression services?

A: It takes 6-10 weeks on average to execute services of yeast protein expression based on the project complexity and the steps.

Q: What is the workflow for yeast protein expression services?

A: Our yeast protein expression services are the process of gene synthesis, codon optimization, vector generation, expression discovery, solubility studies, amplification expression and purification.

Q: What's the delivery packages of Yeast Protein Expression Services?

A: Delivery of expression evaluation report, plasmid sequencing, host transformation, positive strain screening, SDS-PAGE analysis, protein purification (soluble expression), QC report and target protein quality check report.

Q: How to choose the yeast strain for expression of protein?

A: Standard non-methylotrophic yeast strains include Saccharomyces cerevisiae BJ5464, AH22 and INVSc1, and popular methylotrophic yeast strains include Pichia pastoris GS115, KM71 and X-33 because they are highly cell dense and excellent protein producers.

Q: Can yeast protein expression services be used for mass production?

A: The answer is yes, yeast protein expression services can accommodate everything from single-cell research to large-scale manufacturing.

Q: Does the yeast protein expression service do QC services?

A: Yes, yeast protein expression services, QC (SDS-PAGE/UV), HPLC-SEC purity testing, endotoxin analysis.

Q: Does Yeast Protein Expression Services Have Removal Of Tag Protein?

A: And yes, we offer tag protein removal service to make sure that the final product remains pure and functional.

Q: Is there a protein dispensing/lyophilization service for yeast protein expression service?

A: Yes, we offer protein dispensing and freeze-drying for various customers.

Please note that all services are for research use only. Not intended for any clinical use.

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