Professional Yeast Protein Expression Services

Pichia offers several advantages over E. coli: authentic eukaryotic protein folding including disulfide bond formation, post-translational modifications (glycosylation, phosphorylation), secretory expression for simplified purification, and high cell density cultivation achieving grams per liter yields. E. coli remains excellent for simple proteins and rapid screening, but Pichia is preferred for complex eukaryotic proteins requiring proper tertiary structure.

Standard service takes 6-8 weeks from gene receipt to purified protein delivery. This includes sequence optimization, vector construction, clone screening, fermentation, and purification. Rush services (3-4 weeks) are available for expedited projects. Large-scale production (grams) may extend timelines to 10-12 weeks.

Standard Pichia produces high-mannose N-glycans (Man8-14). For humanized glycosylation, we offer engineered strains producing defined human-compatible glycoforms (Man5, complex glycans). We also provide deglycosylated expression options. Glycosylation requirements should be specified during project consultation.

Yes, we've successfully expressed various membrane proteins in Pichia including GPCRs, channels, and transporters. We use detergent-based solubilization strategies and can provide functional assays upon request. Membrane protein expression typically requires more optimization, and we recommend early consultation for challenging targets.

Every project includes SDS-PAGE analysis, Western blot verification (optional), and mass spectrometry confirmation. Additional QC available: endotoxin testing (LAL), SEC-MALS for aggregation, activity assays, and custom specifications. Certificates of Analysis are provided with each shipment.

With our >95% success rate, expression failures are rare. However, if initial attempts don't yield sufficient protein, we offer optimization services including: alternative secretion signals, promoter switching, different strains, or clone rescreening. Full refund or credit is provided for confirmed expression failures after optimization attempts.

We offer expression at scales from milligrams to kilograms. Shake flask screening (50-2000 mL) is ideal for initial evaluation. Benchtop bioreactors (1-50 L) provide consistent results for research and preclinical studies. Industrial-scale fermentation (100-10,000+ L) is available for commercial production.

Yes, codon optimization is included in our standard service. We optimize gene sequences for Pichia codon usage, adjusting GC content, eliminating cryptic splice sites, and minimizing mRNA secondary structure to maximize expression levels.