Protein Purification Service

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Overview Core Service Offerings Technology Platform and Advantages Project Workflow Case Studies Frequently Asked Questions (FAQs) Contact Us

Overview

Protein Purification Service is an important service in the field of biotechnology. It aims to extract and purify high-purity, biologically active proteins from organisms or protein-containing solutions through a series of complex experimental steps. This process usually includes multiple stages such as rough separation, preliminary purification and fine purification, using various technical means such as extraction, precipitation, centrifugation, chromatography, etc. to remove impurities while maintaining the biological activity of the protein.

Schematic diagram of purification of Nanobodies by anion and cation exchange chromatography(S Liu, et al.,2020)

Protein purification services are widely used in drug screening, structural biology research, genetic engineering and other fields, and can meet customers 'customized needs for protein quantity. For example, we can design different purification schemes based on the properties of the protein to ensure the high quality of the final product. In addition, we also provides services such as purification of recombinant proteins with affinity tags, separation and purification of IgG antibodies, and provides separation and purification services for naturally derived proteins and polypeptides with pre-experiments.

Our protein purification services not only include conventional affinity chromatography, ion exchange chromatography, molecular sieve purification and other methods, but also cover various technologies such as gel filtration chromatography and hydrophobic chromatography to meet the needs of different types of protein purification. Through these advanced technologies and strict quality control systems, we are able to provide customers with high-purity and high-activity protein products to meet scientific and clinical needs.

Core Service Offerings

As a CRO service outsourcing company, it provides Protein Purification Service, which includes the following services:

Antibody purification: Use Protein A/G and other technologies to purify antibodies.
Industrial-grade large-scale purification services: Provide efficient protein purification solutions for large-scale production.
Membrane protein purification services: specifically tailored to the purification needs of membrane proteins.
Tagless protein purification: A technique that allows protein purification without labeling.
Protein sample preparation: Including the entire process from sample extraction to preliminary purification.
Tagged protein purification: Use specific tags to purify proteins.

In addition, the following related services may be included:

Protein expression services: Express specific proteins through recombinant DNA technology.
Protein modification services: Specific chemical modifications to proteins to enhance their function or stability.
Quality control and verification services: Ensure that every step meets strict quality standards.
Together, these services constitute a comprehensive protein purification solution designed to meet customer needs in areas such as drug discovery, biotechnology research and diagnostics.

Technology Platform and Advantages

As a CRO service outsourcing company, our company provides protein purification services. The main technology platforms include the following:

High-throughput protein expression, purification and crystallization technology platform: This platform integrates the most advanced technologies and methods in the world, including automated liquid processing workstations, automated cloning and expression plasmid preparation robot systems, automated protein expression, purification and detection robot systems, etc.
High-density fermentation and ultra-clean purification platform: It has a number of key technologies, including integrated high-throughput protein purification technology, ultra-low residue removal technology, ultrafiltration concentration technology, etc.
Two-dimensional gel electrophoresis technology: suitable for protein purification, identification and detection applications, can separate and display thousands of proteins at the same time.
Chromatographic separation technology: including liquid chromatography, ion exchange chromatography, affinity chromatography, reversed phase chromatography, size exclusion chromatography, etc.

These technology platforms can meet the diverse needs of different customers for protein quantity and provide high-quality protein preparation and purification services.

Project Workflow

As a CRO service outsourcing company, our protein purification service process is as follows:

Pretreatment: Proteins need to be released from tissues or cells first. Commonly used methods include physical grinding, ultrasonic disruption, osmotic treatment and cleavage using protease K.
Rough extraction: After the cells are disrupted, select appropriate reagents to dissolve the protein, and remove cell debris and other insoluble matters through centrifuge and other methods. In the rough separation stage, ammonium sulfate precipitation is usually used to separate the target protein from other cellular components such as RNA and DNA.
Fine purification: After rough extraction, enter the fine purification stage. A variety of purification techniques can be used at this stage, such as ion exchange chromatography, affinity chromatography, hydrophobic chromatography, and gel filtration chromatography. These methods are based on the specific properties of the protein, such as charge, affinity and molecular size, to further improve purity and maintain its biological activity.
Quality testing: During the purification process, a comprehensive protein purification analysis report and protein quantity testing report are needed to ensure the high quality of the final product.
Tailored for special needs: Provide scientific research-level and GMP-level protein preparation services according to customers 'different needs to ensure that the needs of scientific research and clinical applications are met.

Through the above process, we can provide customers with a full range of services from protein extraction to fine purification, and ensure that every step meets high standards of quality management requirements.

Case Studies

Case 1: Recombinant protein expression and purification services

Title: Recombinant protein expression and purification services-a one-stop service from gene synthesis and vector construction to protein expression and purification

Content: This case describes in detail the entire process from gene synthesis, vector construction to protein expression and purification. Specifically, the molecular weight of the recombinant protein expressed by HEK293 cells was 43kDa, which was in line with expectations. Using Ni-NTA purification technology, the total amount of purified recombinant protein was 4.5 mg, and the protein purity reached 85%.

Case 2: Mammalian protein expression case

Title: Mammalian Protein Expression Cases-Recombinant Protein Expression Technical Services-Zhongding Biotechnology

Content: This case is based on the pAZV5 secretory expression system, using gene synthesis method to build a pAZV5 expression vector and transiently transfect HEK293 suspension cells. The expression of the protein was detected by SDS-PAGE and Western Blot, and the high-purity recombinant protein was obtained by affinity purification on Ni column.

Frequently Asked Questions (FAQs)

In order to help customers better understand protein purification services, the following are some common questions and their answers:

Q: What should I do if the protein solubility is low?

A: You can try to adjust the pH value, ionic strength of the buffer or add auxiliary dissolving agents (such as urea, glycerol) to improve the solubility of the protein.

Q: Why was my protein inactivated during the purification process?

A: Protein inactivation may be due to shear forces, temperature changes, pH changes, or certain chemical substances. Ensure that mild conditions and appropriate protective measures are used.

Q: What should I do if I encounter non-specific binding problems during protein purification?

A: You can try to optimize the composition of the buffer, add elution steps, or try different affinity column types to reduce non-specific binding.

Q: What should I do if I encounter a column blockage problem during protein purification?

A: Column clogging may be caused by impurities in the sample. You can try cleaning the columns or replacing them with new ones.

Q: How to choose the appropriate protein purification method?

A: Factors such as protein properties (such as solubility, affinity, isoelectric point, molecular weight), purity requirements and cost should be considered when selecting a protein purification method. Commonly used purification methods include affinity chromatography, ion exchange chromatography, molecular sieve, salting-out, etc.

Q: How to optimize protein purification conditions?

A: Methods to optimize protein purification conditions include adjusting the pH, ionic strength, temperature, flow rate and other parameters of the buffer, using different chromatography media and elution buffers, etc.

Q: How to deal with the problem of impurity bands during protein purification?

A: If there are many protein heterobands purified by His tags, you can try to add multiple protease inhibitors or increase the affinity of the heteroprotein to the nickel column.

Q: What are the quality control standards after protein purification?

A: The minimum purity standard we guarantee is>85%. If the initial purification does not meet this standard or the customer has higher purity requirements, we also have the technology for further purification.

Q: How to deal with turbidity during protein purification?

A: The turbidity of the protein may be due to impurities or denaturant residues in the sample. Impurities can be removed by centrifugation, filtration, or replacing fresh buffer.

Q: How to deal with the brown problem of nickel columns during protein purification?

A: The brown appearance of the nickel column may be due to metal ion contamination. You can try cleaning the nickel column or replace it with a new one.

Contact Us

We are a professional CRO service outsourcing company focusing on providing high-quality protein purification services. Our team has rich industry experience and technical strength, and is committed to providing customers with full or partial process services from drug development to marketing. By working with us, you can significantly shorten new drug development time and save a lot of research and development expenses.

If you have any needs or questions about protein purification services, please feel free to contact us. Our customer service team will be happy to serve you and ensure the smooth progress of your project.

Please note that all services are for research use only. Not intended for any clinical use.

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