Rat AKI Array (7 targets)

Catalog Number: AKAA072

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Specifications

Product Information
Product Name	Rat AKI Array (7 targets)
Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Analytes	TIM-1 (KIM-1) FABP1 MCP-1 (CCL2) Cystatin C Lipocalin-2 (NGAL) TIMP-1 VEGF-A
ASSAY SIZE	2 Arrays/4 Arrays/8 Arrays/
Product Features	Easy to useNo specialized equipment neededCompatible with nearly any liquid sampleProven technologyHighly sensitive (pg/ml)Sandwich ELISA specificityHigher density than ELISA, Western blot or bead-based multiplex
Kit Components	Rat Acute Kidney Injury (AKI) Antibody Array C1 MembranesBlocking BufferWash Buffer 1Wash Buffer 2Biotinylated Detection Antibody CocktailStreptavidin-Conjugated HRPDetection Buffer CDetection Buffer DLysis Buffer8-Well Incubation TrayPlastic SheetsArray TemplatesOther Materials RequiredPipettors, pipet tips and other common lab consumablesOrbital shaker or oscillating rockerTissue Paper, blotting paper or chromatography paperAdhesive tape or Saran WrapDistilled or de-ionized waterA chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	1.Block membranes2.Incubate with Sample3.Incubate with Biotinylated Detection Antibody Cocktail4.Incubate with HRP-Conjugated Streptavidin5.Incubate with Detection Buffers6.Image with chemiluminescent imaging system7.Perform densitometry and analysis
Species	Rat
Number of Targets Detected	7
Gene Symbols	CCL2, CST3, FABP1, HAVCR1, LCN2, TIMP1, VEGFA
Compatible Sample Types	Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle	Sandwich-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Storage	For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Please note that all services are for research use only. Not intended for any clinical use.

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