Cas9 Expressing Rat osteosarcoma cells

Catalog Number: GEC0007

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Specifications

Product Information
Product Name	Cas9 Expressing Rat osteosarcoma cells
Specification	1*10^6
System	Bones, joints, soft tissues, skin systems
Resistance	hygro
Cas9 expression detection	Provided
Cell name	UMR-106-CAS9
Cell morphology	Epithelial like, adherent cells
passage ratio	1：2~1：4
Species expression gene	Rat
Expressed Gene	Cas9

Properties
Construction method	Viral method
Mycoplasma detection	negative
Storage and transportation	Dry ice transportation; Store in liquid nitrogen
Culture system	90% DMEM+10% FBS

Overview

UMR-106-CAS9 is a biologically engineered cell line derived from mouse embryonic fibroblasts specifically designed for advanced CRISPR/Cas9-mediated genome editing This unique cell line expresses the Cas9 nuclease under the control of a tightly regulated promoter facilitating targeted gene editing with enhanced efficiency and precision UMR-106-CAS9 cells are cultured in DMEM supplemented with 10% fetal bovine serum (FBS) providing robust nutritional support for cell proliferation and viability

The incorporation of a puromycin resistance gene ensures the selective culture and maintenance of cells expressing Cas9 thereby simplifying the process of obtaining a homogenous population for experimental use Moreover through rigorous validation methods the stable expression of Cas9 is confirmed establishing UMR-106-CAS9 as a reliable tool for researchers engaged in genetic modifications

One of the standout applications of UMR-106-CAS9 is its utility in functional genomics studies Researchers can leverage the gene-editing capabilities of Cas9 to create knockouts knock-ins or specific point mutations allowing for comprehensive investigations into gene function and regulatory mechanisms This feature is particularly valuable in the context of studying complex biological systems disease models and transgenic organism development

Furthermore UMR-106-CAS9 cells serve as an essential platform for the development and validation of innovative therapeutic strategies By utilizing this cell line scientists can assess the therapeutic potential of candidate compounds and gene therapies aimed at curing genetic disorders cancer and other diseases The scalability of UMR-106-CAS9 accompanied by standardized culturing practices supports high-throughput screening initiatives in drug discovery and development

UMR-106-CAS9 cells are mycoplasma-free ensuring the integrity of experimental results and providing peace of mind to researchers They are stored in liquid nitrogen with shipping in dry ice to maintain optimal viability during transit and are shipped at a density of 1×10^6 cells per vial With recommended passaging practices of 1:2 to 1:4 UMR-106-CAS9 cells are optimized for consistent performance across multiple experiments

In summary the UMR-106-CAS9 cell line is an invaluable resource for academic and commercial laboratories alike offering a reliable and efficient platform for genome editing functional assays and the exploration of genetic pathways paving the way for innovative advancements in molecular biology and therapeutic research

Application

UMR-106-CAS9 is primarily utilized in gene editing applications within research and clinical laboratory settings This highly efficient CRISPR-associated protein enables precise modifications of genomic DNA making it invaluable for functional genomics studies disease model creation and therapeutic development Researchers commonly implement UMR-106-CAS9 in various experimental contexts such as generating knockout models in cell lines or organisms conducting targeted gene modifications and enhancing the efficiency of transgenics

In workflows researchers can easily incorporate UMR-106-CAS9 by designing specific guide RNAs targeting the gene of interest followed by delivery via electroporation or lipofection into cultured cells The practical benefits of using UMR-106-CAS9 include reduced off-target effects high specificity and rapid editing efficiency facilitating shorter experimental timelines This product excels in protocols involving multiplexed gene editing where simultaneous targeting of multiple genes is required thus expanding its utility in high-throughput applications and systems biology studies

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Please note that all services are for research use only. Not intended for any clinical use.

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