Reactive Oxygen Species (ROS) Assay Kit - Fluorometric

Catalog Number: AKAS0517

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Specifications

Product Information
Product Name	Reactive Oxygen Species (ROS) Assay Kit - Fluorometric
Instruments	Microplate Reader Fluorescence Microscope Flow Cytometer
Sample Type	Fresh Tissue, Cultured Cells
Research Area	Oxidative Stress Reactive Oxygen Species
Detection Principle	DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Assay Time	60 min
Storage	Reagent 1:-20°C;, Others:2-8°C
Shipping Conditions	Gel Pack

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Please note that all services are for research use only. Not intended for any clinical use.

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