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Overview of protocols of Golden Gate cloning for assembly of multiple DNA fragments.

DNA Assembly and Cloning

DNA assembly and cloning are critical techniques in synthetic biology that enable researchers to create and modify DNA sequences for various applications, including gene editing, protein engineering, and synthetic biology. Traditional cloning methods, such as restriction enzyme digestion and ligation, can be time-consuming and often result in unwanted mutations or errors. However, recent advancements in DNA assembly and cloning techniques have enabled researchers to construct complex DNA sequences with greater speed, accuracy, and efficiency.

Potential uses of synthetic genetic circuits.

Genetic Circuits Design

Genetic circuits are complex systems of interacting genes that can be designed to perform specific functions, such as producing valuable chemicals or sensing environmental signals. The field of synthetic biology has enabled the creation of increasingly sophisticated genetic circuits, which has great potential for various industries. CD Biosynsis provides a wide range of services for genetic circuits design in this field.

The abstract formation of an orthogonal DNA replication system by Plasmid/DNA polymerase pair.

Orthogonal Genetic Systems in Strains

In recent years, the field of synthetic biology has made significant advancements in developing new genetic systems that allow for precise and efficient control of gene expression and editing. One promising approach is the use of orthogonal genetic systems, which enable the simultaneous regulation and editing of multiple genes with high specificity and efficiency. CD Biosynsis is leading the way in providing cutting-edge services in this exciting orthogonal genetic systems field.

Biocontainment strategies and biosafety guidelines.

Biocontainment Strategies in Strains

In the field of synthetic biology, genetically modified organisms (GMOs) have been engineered to produce a variety of products at an industrial scale. However, concerns have been raised surrounding the ecological risks associated with the release of GMOs outside of controlled environments. Biocontainment systems are needed to neutralize these GMOs and prevent them from causing harm to the environment. In addition, alternative selection markers are needed to replace costly and risky antibiotics.

Key advantages of cellular and cell-free bioproduction.

Cell-Free Systems

Cell-free systems are gaining popularity as a platform for protein synthesis due to their rapid and efficient nature. These systems are free from cell walls and membranes and can be used to synthesize proteins directly from DNA templates. They offer several advantages over traditional methods of protein expression, including increased speed, simplicity, and cost-effectiveness. Synthetic biology has played a significant role in advancing cell-free systems by providing new methods for DNA synthesis and assembly, as well as new enzymes and substrates for protein synthesis.

A whole-cell biocatalytic strategy was developed to acylate esculin, and the introduction of medium- and long-length fatty acids chains in acylation endowed esculin with non-selective cytotoxicity.

Whole-Cell Biosynthesis

Whole-cell biosynthesis is a rapidly growing field in synthetic biology that involves the use of living cells as a platform for the production of valuable compounds. This approach offers a number of advantages over traditional chemical synthesis, including improved efficiency, reduced waste, and the ability to use renewable resources. CD Biosynsis is a leading company in the field of whole-cell biosynthesis, providing a range of services for the production of a wide variety of compounds using genetically modified microorganisms.

The non-recombinant approach to display antigens and enzymes on the surface of bacterial spores.

Spore Engineering

Spore engineering is a cutting-edge technology that has revolutionized the field of synthetic biology. It involves the genetic modification of bacterial spores, which are highly resistant structures that can survive harsh environmental conditions. Spore engineering has various applications in the fields of bioremediation, bioproduction, drug delivery, and biosensing. CD Biosynsis is a leading company in spore engineering, providing innovative solutions to meet the needs of our clients.

Elimination of the apramycin resistance gene in the host strain genome.

Antibiotic Resistance Marker Removal

The use of antibiotic resistance genes as selection markers in synthetic biology strains has been a common practice for many years. However, the increasing concern over the spread of antibiotic resistance has prompted scientists to develop alternative selection systems. The use of antibiotic-free selection systems is not only a safer option but also more cost-effective in the long run.

Schematic diagram of the biodegradation of toxic recalcitrant compounds.

Biodegradation Pathway Engineering

Synthetic biology has emerged as a promising approach for engineering microorganisms to perform complex metabolic tasks, including biodegradation. Biodegradation pathway engineering involves the modification of microbial metabolism to degrade specific contaminants or pollutants. At CD Biosynsis, we offer a range of services for biodegradation pathway engineering, utilizing the latest synthetic biology tools and techniques to design and optimize microbial consortia for biodegradation applications.

Custom Cell Line Development Services

Custom cell line development services offer specialized solutions for creating and optimizing cell lines tailored to specific research, therapeutic, or biotechnological needs. Our services provide comprehensive support from initial consultation to final validation, ensuring that your custom cell lines meet your precise requirements for functionality, stability, and reproducibility.

Figure 1. Synthetic biology research cycle for the development of new biological systems. (Linshiz G, et al., 2016)

Biosynthesis Data Analysis

CD Biosynsis provides high-quality data analysis services for the learn phase of synthetic biology to help researchers around the world facilitate their cutting-edge research. We have the ability and extensive experience to help bring diverse synthetic biology applications and creative ideas to fruition.

Major aspects of the de novo protein design.

De Novo Protein Design

De novo protein design is revolutionizing synthetic biology by enabling the creation of tailor-made proteins with unprecedented functions. Our services empower industries and researchers to harness the potential of these engineered biomolecules for diverse applications, from therapeutics to materials science.

Assay design for high-throughput screening.

Strain Hight-throughput screening

Our high-throughput screening services are dedicated to assisting researchers and organizations in achieving their scientific goals efficiently and effectively, leveraging state-of-the-art technology and expertise to accelerate progress in diverse fields of study.

Fig 1. Sustainable production of chemicals by combining industrial process optimization and genetic engineering approaches.

Chemical production Metabolic pathways Engineering

CD Biosynsis is committed to providing high-quality services to our clients. Our specialized synthetic biology platform specializes in providing tools, libraries of gene parts for metabolic pathways, and bioengineering for chemical production, as well as designing and optimizing metabolic pathways for maximum efficiency.

Figure 1. Integrated design-build-test-learn cycles in synthetic biology encompassing whole-cell model-guided approaches, and relative applications. (Marucci L, et al., 2020)

Biosynthesis Experimental Redesign

CD Biosynsis provides experimental redesign services for the "learn" phase of synthetic biology to help researchers around the world facilitate their cutting-edge research. We have the ability and extensive experience to help bring diverse synthetic biology applications and creative ideas to fruition.

Automation Screening

Strain Automation screening

Automation screening is a pivotal technology in synthetic biology, revolutionizing research across various domains. By harnessing the power of automation, CD Biosynsis' services contribute to the advancement of life sciences, enabling rapid, systematic, and high-throughput analysis that was previously unattainable through manual methods.

Fig 1. Direct combinatorial pathway optimization.

Biosynthesis Pathway design and optimization services

CD Biosynsis has expertise in pathway engineering, computational tools, and collaborative approaches that are well suited to meet the diverse needs of our clients in synthetic biotechnology.

Service process flow for site-directed mutagenesis at CD Biosynsis.

Site-Directed Mutagenesis

Site-directed mutagenesis is a very useful in vitro technique to create specific, targeted mutations in a known DNA sequence. It can efficiently change the characterization of target proteins. Site-directed mutagenesis can be used as a precision tool to enable synthetic biology. It plays an essential role in a variety of synthetic biology research, such as the study of gene regulatory elements, protein structure and functions, enzyme active sites and novel proteins.

In silico screening of peptide from edible peptide database.

Strain In silico screening

In silico screening is revolutionizing synthetic biology by offering a powerful and cost-effective approach to biological research and drug discovery. Our comprehensive services, coupled with cutting-edge technology and expert guidance, position us at the forefront of this transformative field. Unlock the full potential of your research with our In Silico Screening Services.

Figure 1. Comparison of various commercial recombinant protein expression systems. (Raskin I, et al., 2002)

Cell-Based Protein Expression

CD Biosynsis has a team of experienced and highly skilled scientists to provide synthetic biology researchers around the world with high-quality cell-based protein expression services to facilitate their cutting-edge research.

Metabolic Pathway Synthesis and Optimization

Metabolic Engineering

Metabolic engineering and synthetic biology have combined to become one of the most promising fields. CD Biosynsis provides a comprehensive portfolio of metabolic services for the build phase of the Design-Build-Test-Learn (DBTL) cycle to help synthetic biology researchers around the world facilitate their cutting-edge research. We provide powerful tools and tailored solutions to help our customers overcome the challenges in synthetic biology and bring diverse creative ideas to fruition.

Figure 1. Schematic representation of the differences and convergence between synthetic biology and metabolic engineering. (Pouvreau B, et al., 2018)

Plant Metabolic Engineering

CD Biosynsis provides comprehensive services for plant metabolic engineering to facilitate the cutting-edge research of synthetic biology researchers around the world.

The primary lutein biosynthetic pathway.

Biosynthesis Screen assay and characterization

CD Biosynsis provides a comprehensive suite of tools to accelerate your synthetic biology research. By harnessing the power of high-throughput screening and in-depth characterization, we empower researchers and innovators to unlock the full potential of biological systems for a wide range of applications, from drug discovery to biotechnology advancements.

Figure 1. Schematic diagram of the backbone building process at CD Biosynsis.

Protein Backbone Building

CD Biosynsis provides professional backbone building services for synthetic biology researchers around the world to facilitate their cutting-edge research. Our aim is to obtain protein backbone configurations that satisfy a set of predefined requirements by the customers.

Figure 1. Specification and design of a genetic AND gate and dual-feedback oscillator. (Appleton E, et al., 2017)

Design Automation

CD Biosynsis has advanced design automation platforms and extensive experience to help synthetic biology researchers around the world facilitate their cutting-edge research.

Figure 1. A snapshot of synthetic biology tools used for modification of microbial enzymes. (Shukla P, 2019)

Enzyme Engineering

CD Biosynsis provides professional enzyme engineering services to facilitate the cutting-edge research of synthetic biology researchers around the world.

Figure 1. Tools and approaches for studying multicellular behavior. (Mathur M, et al., 2017)

Cellular Analysis

CD Biosynsis provides comprehensive cellular analysis for the test phase of synthetic biology to help researchers around the world facilitate their cutting-edge research. Equipment with an advanced technology platform and powerful synthetic biology tools, we have the ability to help bring diverse synthetic biology applications and creative ideas to fruition.

Various types of epigenetic modifications under stress conditions.

Plant Strain Modification

Plant strain modification, also known as plant breeding or genetic engineering, involves the deliberate alteration of a plant's genetic makeup to achieve specific desired traits. This process aims to improve crop yield, quality, resistance to pests and diseases, and tolerance to various environmental conditions. Plant strain modification is a powerful tool in plant biotechnology that enables the manipulation of plant traits to improve crop yields, enhance disease resistance, and increase nutritional value.

Heterologous reconstitution of plant pathways in yeast, as exemplified by reconstitution of opiate biosynthetic pathways in yeast.

Plant Metabolic Pathway Engineering

Plant metabolic pathway engineering is a powerful tool that has revolutionized the field of synthetic biology. It involves the modification of plant metabolic pathways using genetic engineering techniques to produce high-value compounds, such as pharmaceuticals, industrial chemicals, and biofuels. The use of synthetic biology tools in plant metabolic engineering has enabled the production of these valuable compounds in a cost-effective and sustainable manner.

TMV based plant-derived vaccine for Non-Hodgkins Lymphoma.

Plant Virus-Based Vector Systems

Plant virus-based vector systems have emerged as an efficient and practical tool for the production of recombinant proteins in plants. This technology has revolutionized the field of synthetic biology and has been widely adopted for the production of high-value proteins, such as vaccines, antibodies, and enzymes.

Overview of plant transformation approaches employed for the production of recombinant pharmaceutical and non-pharmaceutical proteins in plants.

Plant Molecular Farming

Plant molecular farming is a rapidly growing field in synthetic biology that utilizes plants as bioreactors for the production of recombinant proteins. This innovative approach presents many advantages over traditional mammalian-based expression systems, such as lower costs, reduced risk of contamination, and increased scalability. At CD Biosynsis, we specialize in providing high-quality services for plant molecular farming, leveraging our expertise in synthetic biology and plant biotechnology to offer customized solutions for our clients.

Experimental Design

Biosynthesis Experimental Design

CD Biosynsis provides one-stop experimental design services to help synthetic biology researchers around the world to facilitate their cutting-edge research.

Synthetic biology approach to develop chassis microbe strain.

Strain Screening and Characterization Services

Strain characterization and analysis are crucial steps in understanding the properties and behavior of microorganisms. Accurate characterization of strains is essential in various fields such as agriculture, biotechnology, medicine, and environmental science. At CD Biosynsis, we offer a range of services for strain characterization and analysis, including phenotypic characterization, genotypic analysis, and bioinformatics analysis.

High-throughput Strain Screening Services

High-throughput strain screening services provide advanced solutions for rapidly identifying and optimizing microbial strains with desirable traits for research and industrial applications. This technology enables the efficient screening of large libraries of microbial strains to discover those with superior performance in various processes such as fermentation, bio-production, and metabolic engineering. Our comprehensive services offer support from initial library construction to final strain selection, ensuring precise and reliable results for your screening projects.

Phenotypic characterization of strains bearing MNase-tagged TFs.

Strain Phenotypic Characterization

Phenotypic characterization of strains is a fundamental aspect of microbiology. It involves the identification and characterization of microorganisms based on their observable traits, such as colony morphology, growth rate, and biochemical properties. This information is essential for the diagnosis and treatment of infectious diseases, the development of new drugs, and the study of microbial ecology and evolution.

Strain identification process.

Strain Authentication Service

As the field of synthetic biology continues to evolve, the need for reliable and accurate strain authentication methods has become increasingly important.

Overview of next-generation sequencing-assisted genetic analysis of epilepsy disorders.

Strain Genotypic Analysis

Genotypic analysis plays a pivotal role in advancing our understanding of genetic variations and their impact on various biological processes. At CD Biosynsis, a leading company specializing in synthetic biology, we are committed to providing cutting-edge services in genotypic analysis. With years of experience in the industry, our team of highly proficient biological experts combines their expertise with state-of-the-art technologies to deliver accurate and comprehensive genotypic analysis solutions.

chematic representation of the conformational stability assay (CSA).

Strain Stability Testing

In the field of synthetic biology, ensuring the stability and genetic integrity of strains is of paramount importance. Genetic stability testing plays a crucial role in verifying the reliability and safety of engineered strains used in various applications, including vaccine production, biomanufacturing, and bioengineering.

Hierarchical Cluster Analysis (HCA) based on LC-MS metabolic profiles from the organic extracts of 24 bacterial strains and TSB culture media.

Strain Metabolic Profiling

Metabolic profiling, also known as metabolomics, is the large-scale study of metabolites present in cells, tissues or biofluids. Metabolites are small molecules such as amino acids, sugars, and lipids that are the end products of cellular processes. By measuring changes in metabolites, researchers can gain a snapshot of an organism's biochemical state and how it responds to stimulus. Metabolic profiling is thus a powerful tool for characterizing biological systems, identifying disease biomarkers, and studying drug effects and toxicities.

Figure 1. Bacterial surface display of antigens. (Glass D S & Riedel-Kruse I H, 2018)

Bacterial Cellular Engineering

CD Biosynsis provides comprehensive bacterial cellular engineering services to facilitate the cutting-edge research of synthetic biology researchers around the world.

Figure 1. The design–build–test–learn cycle of metabolic engineering highlighting important parts of each of the components. (Petzold C J, et al., 2015)

Metabolite Analysis

CD Biosynsis provides a wide range of metabolite analysis services for the test phase of synthetic biology to help researchers around the world facilitate their cutting-edge research. Equipment with an advanced technology platform and powerful synthetic biology tools, we have the ability to help bring diverse synthetic biology applications and creative ideas to fruition.

CRISPR‒Cas9-based genome-editing approaches.

Genetic Engineering

Genetic engineering has revolutionized various industries, including healthcare, agriculture, and biomanufacturing. The field of synthetic biology, which involves the design and construction of new biological systems or redesigning existing ones, has further expanded the possibilities of genetic engineering. Genetic engineering has been a rapidly evolving field over the past few decades, with the development of new technologies and techniques that have made it possible to manipulate genes with unprecedented precision and efficiency. The potential of genetic engineering to revolutionize various industries has been well-established, but it also raises ethical and regulatory challenges that need to be addressed.

Metabolic Pathway Optimization in Strains

Strain Pathway Optimization

Synthetic biology is a rapidly growing field that has revolutionized the way we think about the production of biological compounds. One of the key challenges in synthetic biology is the optimization of metabolic pathways in strains to produce high-value compounds. Metabolic pathway optimization involves the design, engineering, and optimization of the enzymatic and regulatory processes that control the production of specific compounds in microbial cells. However, the complexity of cellular metabolism and the limited understanding of the underlying mechanisms make the optimization of metabolic pathways a tedious and time-consuming process. Therefore, new approaches are required to accelerate the pathway optimization process and improve the efficiency and accuracy of the metabolic engineering process.

Diagram of modular plasmid design.

Plasmid Design and Transformation

Plasmids are small, self-replicating circular DNA molecules that play a crucial role in synthetic biology. They are used to introduce genes of interest into host cells, allowing for the manipulation and engineering of biological systems.

DNA Assembly and Cloning Services

DNA Assembly and Cloning Services

Through DNA assembly and cloning services, CD Biosynsis aims to provide researchers and innovators with streamlined access to complex genetic constructs, which will alleviate the technological challenges associated with DNA manipulation and enable researchers to focus on their core objectives, facilitating their breakthroughs in different fields.

Fig 1. The main stages of the database development - a clinical example.

Biosynthesis Database building

CD Biosynsis provides high-quality database building services for the "learn" phase of synthetic biology to help researchers around the world facilitate their cutting-edge research. We have the ability and extensive experience to help bring diverse synthetic biology applications and creative ideas to fruition.

Fig 1. Digital Experimentation and DOE.

Biosynthesis Digital experimeand and DOE

In recent years, the integration of digital experimentation (DE) and design of experiments (DOE) services has revolutionized the design phase of the DBTL cycle, enhancing efficiency and accelerating innovation. CD Biosynsis is dedicated to providing professional digital experimentation and DOE services to help researchers around the world advance their cutting-edge research.

Graphical abstract of evaluation of strains.

Strain Performance Evaluation

In the rapidly advancing field of synthetic biology, the performance evaluation of strains plays a pivotal role in optimizing biological systems for various applications. Synthetic biology combines principles from biology, engineering, and computer science to design and construct new biological parts, devices, and systems with enhanced functionalities.

Graphical abstract of UV-Vis process monitoring.

Strain Fermentation Monitoring

Fermentation is a fundamental biological process that has been used for centuries to produce a wide range of products, including food, beverages, pharmaceuticals, and biofuels. Traditional methods of fermentation monitoring involve time-consuming and costly methods such as manual sampling and offline analysis. However, monitoring the fermentation process in real-time can be challenging due to the complex interactions between microorganisms, substrates, and environmental factors.

Figure 1. Large-scale reengineering of organisms. (Standage-Beier K & Wang X, 2017)

Genome Reprogramming Service

CD Biosynsis provides custom genome reprogramming services for synthetic biology researchers around the world to facilitate their cutting-edge research. Our strong expertise coupled with advanced techniques can help bring diverse synthetic applications and creative ideas to fruition.

The workflow of protein structure prediction at CD Biosynsis.

Biosynthesis In Silico and Experimental Validation

CD Biosynsis provides professional in silico and experimental validation services for synthetic biology researchers around the world to facilitate their cutting-edge research.

Genes chosen for modification and their positions in metabolic fluxes.

Strain Metabolic Engineering

Metabolic engineering is a rapidly growing field that involves the design and construction of microbial cells for the production of high-value compounds. The optimization of metabolic pathways is a crucial step in this process, as it can significantly improve the efficiency and accuracy of production.

Time-resolved extracellular metabolite concentrations (mmol/l) of COL and HG001 were visualized with MeV as color coded chart.

Strain Metabolic Throughout Analysis

Synthetic biology involves engineering biological systems through the design and construction of new biological parts, devices, and systems, or the modification of existing ones. One of the key challenges in synthetic biology is to create efficient and reliable metabolic pathways in microbial strains.

Novel CRISPR System

Novel CRISPR Gene Editing Service

In a world where scientific breakthroughs hinge on innovative tools, our novel CRISPR system stands at the forefront of genetic engineering innovation. With its precision, versatility, and diverse applications, it empowers researchers and industry leaders to push the boundaries of what's possible in the realm of synthetic biology.

Fig 1. Unbiased libraries in protein directed evolution.

Protein library design

CD Biosynsis's synthetic biology platform is dedicated to providing high-quality protein library design services to help biologists around the world design protein variant collections to support innovation in a variety of fields.

Flux-dependent graphs for metabolic networks.

Strain Metabolic Network Modeling

Metabolic network modeling is a powerful tool for understanding the complex interactions between biological systems and their environments. It involves the use of computational techniques to analyze and simulate the metabolic pathways in organisms, with the goal of predicting their behavior under different conditions. In recent years, metabolic network modeling has become increasingly important in synthetic biology, where it is used to design and optimize strains for a wide range of applications.

Components of precision engineering technology for microbial strain improvement.

Strain Improvement Service

Strain improvement is a critical aspect of synthetic biology that involves the targeted enhancement of microorganisms or cell lines for specific applications. It revolves around modifying the genetic, metabolic, and physiological characteristics of these organisms to optimize their performance in various industrial, medical, or research contexts.

General workflow for selection and high-resolution phenotypic screening of bacterial mutants in the nwSlide.

Strain Phenotypic Screening and Selection

Phenotypic screening and selection is a crucial step in the development of novel strains with improved performance in synthetic biology. With the increasing demand for high-value products and sustainable solutions, there is a growing need for efficient and effective screening methods that can identify the most promising strains for further development. At CD Biosynsis, we offer a range of services for phenotypic screening and selection, utilizing cutting-edge technologies and expertise in synthetic biology to deliver the best possible results for our clients.

The role of RapP in the domestication of strain 168.

Strain Domestication Service

Microbial strains play a significant role in the production of various industrial compounds, such as biofuels, chemicals, and pharmaceuticals. However, the performance of these strains is often limited by their natural diversity, making it challenging to achieve high production yields and efficiency. To overcome these limitations, researchers have turned to synthetic biology and strain domestication as promising strategies for improving microbial strains.

Applications of adaptive laboratory evolution (ALE) in strain development for biomanufacturing and in basic science research.

Strain Adaptive Evolution

Adaptive evolution is a powerful tool used in synthetic biology to improve the performance of microbial strains for various biotechnological applications. The technique involves the selection and propagation of genetic variants with desirable traits over multiple generations, resulting in the evolution of the microbial population towards a specific objective. One of the significant advantages of adaptive evolution is that it can be used to modify a strain's genome without the need for genetic engineering. This approach allows for the optimization of a strain's performance by selecting for natural genetic variation that confers a selective advantage under specific growth conditions.

DNA mutation mechanisms by physical and chemical mutagenesis.

Strain Mutagenesis Service

Mutagenesis is a powerful tool that enables researchers to generate genetic diversity in microorganisms and other organisms. It involves the induction of mutations in the genome of an organism, which can result in the generation of novel phenotypes that may have beneficial properties for various applications, including industrial biotechnology. Synthetic biology has been instrumental in the development of novel methods for mutagenesis that enable the generation of large libraries of mutants with diverse phenotypes.

The principles of in situ hybridization chain reaction.

Strain Hybridization Service

One of the key components of synthetic biology is the creation of novel strains with unique properties that can be used for various applications such as biotechnology, medicine, and agriculture. Strain hybridization is an essential technique in synthetic biology that involves the combination of genetic material from different strains to create a new hybrid strain with desired traits.

DNA vector construction service

DNA vector construction service

DNA vector construction is a fundamental technique in molecular biology that involves creating recombinant DNA molecules for various applications. It plays a crucial role in genetic engineering, gene therapy, and biotechnology research. By designing and assembling DNA sequences into a vector, which acts as a carrier, DNA vector construction enables the delivery of desired genetic material into target cells or organisms. This process is essential for manipulating and studying genes, as well as for developing new treatments and therapies.

Figure 1. A conceptualized pathway design workflow. (Wang L, et al., 2017)

Biosynthesis Pathway Design

CD Biosynsis provides comprehensive pathway design services for synthetic biology researchers around the world to facilitate their cutting-edge research.

The mechanisms of genome editing and DSB repair.

Strain Gene Editing

Synthetic biology is a rapidly growing field that has the potential to revolutionize many industries, including biotechnology, medicine, and agriculture. One of the key tools in synthetic biology is gene editing, which allows scientists to manipulate the DNA of organisms to create new functions, optimize metabolic pathways, and engineer new traits. In recent years, the development of new gene editing tools, such as CRISPR-Cas9, has made gene editing faster, cheaper, and more precise than ever before.

Codon frequencies in human

Codon optimization service

Codon optimization is a crucial technique used to strategically modify the genetic code of a gene in order to enhance protein expression in a specific host organism. By fine-tuning codon usage, we can significantly improve protein production yield, enhance protein functionality, and increase overall efficiency.

Plasmid DNA Preparation Service

Plasmid DNA Preparation Service

Plasmid DNA Preparation is a crucial process in molecular biology that involves isolating and purifying plasmid DNA from bacterial cells. Plasmids are small, circular DNA molecules commonly used in genetic engineering and research. Plasmid DNA Preparation ensures the production of high-quality and pure DNA samples for various applications such as cloning, gene expression studies, and genetic engineering experiments.

PCR Cloning

PCR Cloning Service

PCR Cloning is a powerful molecular biology technique that allows scientists to amplify a specific DNA sequence and insert it into a vector for further study or manipulation. This method has revolutionized the field of genetic engineering by enabling the creation of multiple copies of DNA fragments, which can be used for various applications in molecular biology and biotechnology.

(CF Peddle, et al.,2017)

sgRNA Design Service

SgRNA (short guide RNA) design is the process of designing RNA molecules for the CRISPR-Cas9 genome editing tool. The CRISPR-Cas9 system is composed of Cas9 protein and guiding RNA (sgRNA), which can accurately cleave specific DNA sequences, thereby achieving genome editing and repair.

CRISPR/Cas9 genome editing system(Y Cui, et al.,2018)

CRISPR-Cas9 sgRNA synthesis

CRISPR-Cas9 sgRNA synthesis refers to the use of molecular biology techniques to synthesize sgRNA (single guide RNA) molecules in the CRISPR-Cas9 system. CRISPR-Cas9 is a technology used for gene editing that can accurately modify DNA sequences. SgRNA is a key component of the CRISPR-Cas9 system, which can guide Cas9 enzymes to locate target DNA sequences and perform cleavage or repair. Therefore, synthesizing sgRNA is a necessary step for conducting CRISPR-Cas9 gene editing experiments. The synthesis of sgRNA is usually achieved through in vitro synthesis methods, using chemical synthesis or in vitro transcription.

 Engineering protein, metabolism and artificial cell in the open cell-free system.

Biosynthesis Cell free system

CD Biosynsis harnesses the transformative power of the Cell-Free System to catalyze innovation across the biotech landscape. With an unwavering focus on precision, quality, and versatility, we empower researchers and biotech entities to explore new horizons and unlock the full potential of molecular biology. Join us on the journey to redefine the future of biotechnology through our pioneering Cell-Free System Services.

Biosynthetic gene cluster and proposed biosynthetic pathway of 1.

Biosynthesis Gene Cluster

CD Biosynsis specializes in Biosynthesis of gene cluster services, offering cutting-edge solutions for synthetic biology applications. Our comprehensive services encompass gene cluster analysis, design, and optimization to drive innovation in biotechnology.

Schematic representation of cell-free methodology modification for the production of diverse proteins.

Protein Biosynthesis

CD Biosynsis specializes in cutting-edge protein biosynthesis services. Leveraging synthetic biology techniques, we deliver precise and efficient protein synthesis solutions tailored to your unique research and industrial needs.

Salmonella Genome Editing Service

Our Salmonella Genome Editing Service offers precise and efficient solutions for genetic modifications in Salmonella species, optimizing them for use in research, vaccine development. Utilizing state-of-the-art genome editing technologies such as CRISPR/Cas9, we provide comprehensive support from project design to final validation, ensuring your genome editing goals are achieved with high accuracy and efficiency.

Pseudomonas aeruginosa Genome Editing Service

Our Pseudomonas aeruginosa Genome Editing Service offers precise and efficient solutions for genetic modifications in Pseudomonas aeruginosa, optimizing them for use in research, industrial applications, and medical biotechnology. Utilizing state-of-the-art genome editing technologies such as CRISPR/Cas9, TALENs, and recombineering, we provide comprehensive support from project design to final validation, ensuring your genome editing goals are achieved with high accuracy and efficiency.

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