Ribosome Display Service

Ribosome display allows library sizes up to 10¹⁴ variants, which is 10,000-100,000 times larger than typical phage display libraries (~10⁹-10¹⁰). Additionally, ribosome display is performed entirely in vitro, avoiding cellular viability constraints and allowing selection of toxic proteins.

Most projects require 3-5 rounds of selection to achieve adequate enrichment of target binders. The exact number depends on library complexity, target properties, selection stringency, and desired enrichment levels. Our team will advise on optimal rounds based on your project goals.

Yes! One of the key advantages of ribosome display is that it uses cell-free systems. This means you can select proteins that would be toxic to cells, unstable in vivo, or require specific conditions not compatible with cellular expression.

With iterative selection and affinity maturation, our service can deliver binders with affinities ranging from high nanomolar to picomolar KD values. Final affinity depends on the target properties, library design quality, and selection strategy employed.

Standard QC includes NGS verification of selected hits to confirm diversity and enrichment, ELISA-based binding confirmation against target and counter-targets, and comprehensive sequence analysis. Additional validation such as SPR/BLI kinetics can be provided as add-on services.

Yes, our PURE system-based ribosome display can incorporate non-standard amino acids at specific positions. This enables development of peptidyl therapeutics with enhanced stability, specificity, or novel functions.

We offer four cell-free translation systems: E. coli S30 extract (cost-effective for large libraries), wheat germ extract (excellent for eukaryotic proteins), rabbit reticulocyte lysate (good post-translational modifications), and PURE system (defined components for maximum flexibility).

Membrane proteins can be challenging for traditional display technologies. Our cell-free ribosome display platform can select binders against membrane proteins using detergent-solubilized targets or nanodisc presentations. We optimize presentation conditions to maintain target native structure.

Beyond initial hit identification, we offer: affinity determination by SPR/BLI, epitope mapping, specificity profiling against related targets, cross-reactivity screening, developability assessment, and functional assays.