Saccharomyces cerevisiae Metabolic Engineering Services

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Saccharomyces cerevisiae metabolic engineering services offer advanced solutions for optimizing the metabolic pathways of this widely used yeast species to enhance the production of valuable biochemicals, biofuels, pharmaceuticals, and other industrially relevant products. Our comprehensive services provide support from initial project design to final strain optimization, ensuring precise and efficient metabolic modifications tailored to your specific research and biotechnological needs.

Construction of yeast strains for simultaneous utilization of lignocellulosic sugars (J Lian, et al.,2018)

Overview Service Process Examples and Solutions Case Study Frequently Asked Questions

Overview

Saccharomyces cerevisiae, commonly known as baker's yeast, is a model organism extensively used in genetics, molecular biology, and biotechnology. Its ease of genetic manipulation, well-characterized genome, and ability to thrive in various industrial processes make it an ideal system for metabolic engineering. Our services leverage advanced genetic engineering techniques, including CRISPR/Cas9, homologous recombination, and synthetic biology approaches, to deliver high-quality engineered yeast strains.

The applications of Saccharomyces cerevisiae metabolic engineering are diverse and impactful, including:

Biofuel Production: Enhancing the production of ethanol, butanol, and other biofuels.
Pharmaceutical Production: Engineering metabolic pathways to produce antibiotics, vitamins, and other pharmaceuticals.
Industrial Biochemicals: Producing organic acids, alcohols, and other industrially relevant chemicals.
Food and Beverage Industry: Optimizing yeast strains for the production of flavors, fragrances, and food additives.
Synthetic Biology: Constructing synthetic pathways for the production of novel compounds and materials.
Agricultural Biotechnology: Developing yeast strains that produce biopesticides and plant growth-promoting factors.

Service Process

The process of Saccharomyces cerevisiae metabolic engineering involves several critical and interrelated steps:

Project Consultation: Collaborating with researchers to define specific metabolic engineering goals, including target compounds, desired metabolic modifications, and intended applications.
Pathway Analysis and Design: Analyzing existing metabolic pathways and designing modifications to optimize the production of target compounds. This includes pathway reconstruction and flux balance analysis.
Vector Design and Construction: Designing and constructing expression vectors or CRISPR/Cas9 systems tailored to the specific genetic modifications needed for the metabolic pathway.
Yeast Transformation: Introducing the genetic material into yeast cells using techniques such as electroporation or lithium acetate transformation.
Selection and Screening: Selecting successfully transformed yeast cells using selectable markers and screening for desired metabolic modifications using assays such as HPLC, GC-MS, and enzymatic assays.
Strain Optimization: Optimizing the engineered strains through iterative rounds of modification and selection to enhance the production of target compounds. This may include optimizing growth conditions and media composition.
Characterization and Validation: Characterizing the engineered yeast strains to confirm the presence and functionality of the metabolic modifications. This includes growth assays, metabolic profiling, and functional assays.
Scale-Up and Production: Scaling up the engineered strains for large-scale production and further applications in research or industry.
Reporting and Consultation: Providing a detailed report of the findings and offering further consultation to interpret the results and plan subsequent research steps.

For more information about our Saccharomyces cerevisiae Metabolic Engineering Services or to discuss your specific needs, please contact us. Our team of experts is available to provide guidance and support for your research and biotechnological projects, ensuring you achieve your scientific and industrial goals.

Examples and Solutions

The following table provides an overview of various case studies in Saccharomyces cerevisiae metabolic engineering and the solutions we offer to support your research and biotechnological endeavors:

Case Study	Description	Solutions We Offer
Ethanol Production Optimization	Engineering yeast strains to enhance ethanol production from biomass.	Pathway design, genetic modification, strain optimization, and scale-up.
Antibiotic Production Enhancement	Modifying metabolic pathways to increase the yield of antibiotics.	CRISPR/Cas9 gene editing, pathway optimization, and production scaling.
Organic Acid Production	Developing strains for the efficient production of organic acids for industrial use.	Metabolic pathway reconstruction, strain engineering, and yield optimization.
Biofuel Production Strains	Optimizing metabolic pathways for the efficient production of biofuels.	Gene pathway engineering, strain development, and production optimization.
Industrial Biochemical Synthesis	Engineering yeast to produce solvents and other industrial biochemicals.	Synthetic biology, pathway integration, and functional assays.
Synthetic Pathway Construction	Constructing synthetic pathways in yeast for the production of novel biochemicals.	Synthetic biology, pathway integration, and functional assays.

Case Study

Saccharomyces cerevisiae was engineered with an n-butanol biosynthetic pathway, in which isozymes from a number of different organisms (S. cerevisiae, Escherichia coli, Clostridium beijerinckii, and Ralstonia eutropha) were substituted for the Clostridial enzymes and their effect on n-butanol production was compared. By choosing the appropriate isozymes, we were able to improve production of n-butanol ten-fold to 2.5 mg/L. The most productive strains harbored the C. beijerinckii 3-hydroxybutyryl-CoA dehydrogenase, which uses NADH as a co-factor, rather than the R. eutropha isozyme, which uses NADPH, and the acetoacetyl-CoA transferase from S. cerevisiae or E. coli rather than that from R. eutropha. Surprisingly, expression of the genes encoding the butyryl-CoA dehydrogenase from C. beijerinckii (bcd and etfAB) did not improve butanol production significantly as previously reported in E. coli. Using metabolite analysis, we were able to determine which steps in the n-butanol biosynthetic pathway were the most problematic and ripe for future improvement.

n-Butanol production from engineered S. cerevisiae (EJ Steen, et al.,2008)

Symbols and strains: black squares, ESY7; empty squares, ESY11; black circles, ESY2; the rest of the samples all produced approximately the same amount of n-butanol and are indicated on the graph. Symbols and error bars represent the mean and standard deviation of triplicate cultures.

n-Butanol pathway intermediates at 24 h (EJ Steen, et al.,2008)

Bars and strains: black bars, ESY4; gray bars, ESY7; white bars, ESY11. (A) All pathway intermediates in strains ESY4, 7 and 11. (B) HbCoA, CrCoA and BtCoA intermediates in strains ESY4 and ESY7. Levels of AcCoA were similar except for strains ESY11 (A). Levels of 3-hydroxybutyryl-CoA (HbCoA) and butyryl-CoA (BtCoA) were notably higher in ESY7 compared to ESY4, while crotonyl-CoA (CrCoA) was relatively similar in the two strains. Values and error bars represent the mean and standard deviation of triplicate cultures.

Frequently Asked Questions

Q: What is Saccharomyces cerevisiae metabolic engineering?

A: Saccharomyces cerevisiae metabolic engineering involves the genetic modification of yeast strains to optimize their metabolic pathways for the production of target compounds. This can include introducing, deleting, or modifying specific genes to redirect metabolic fluxes and increase the yield of desired products.

Q: How is Saccharomyces cerevisiae metabolic engineering performed?

A: Saccharomyces cerevisiae metabolic engineering is performed through a series of steps including project consultation, pathway analysis and design, vector design and construction, yeast transformation, selection and screening, strain optimization, characterization and validation, scale-up and production, and reporting. Each step ensures precise and efficient metabolic modifications.

Q: What are the applications of Saccharomyces cerevisiae metabolic engineering?

A: Applications include biofuel production, pharmaceutical production, industrial biochemicals production, food and beverage industry, synthetic biology, and agricultural biotechnology. Engineered yeast strains are used to produce valuable bioproducts and address various industrial and environmental challenges.

Q: What are the key steps in the Saccharomyces cerevisiae metabolic engineering process?

A: Key steps include project consultation, pathway analysis and design, vector design and construction, yeast transformation, selection and screening, strain optimization, characterization and validation, scale-up and production, and reporting. These steps ensure comprehensive and accurate development of engineered yeast strains.

Q: Why is Saccharomyces cerevisiae metabolic engineering important?

A: Saccharomyces cerevisiae metabolic engineering is important for advancing research, developing new bioproducts, optimizing industrial processes, and addressing environmental challenges. Engineered yeast strains provide valuable tools for enhancing production yields and creating novel compounds.

Please note that all services are for research use only. Not intended for any clinical use.

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