Site-Directed Mutagenesis

Site-directed mutagenesis is a very useful in vitro technique to create specific, targeted mutations in a known DNA sequence. It can efficiently change the characterization of target proteins. Site-directed mutagenesis can be used as a precision tool to enable synthetic biology. It plays an essential role in a variety of synthetic biology research, such as the study of gene regulatory elements, protein structure and functions, enzyme active sites and novel proteins.

CD Biosynsis provides professional site-directed mutagenesis services for synthetic biology researchers around the world to facilitate their cutting-edge research. We can provide fast and economical services, including point mutations, deletions, and insertions, to meet the specific needs of our customers.

Service Process

CD Biosynsis provides comprehensive upstream and downstream services for site-directed mutagenesis, including but not limited to template DNA sequencing, gene synthesis, gene cloning, and protein expression and purification.

Figure 1. Service process flow for site-directed mutagenesis at CD Biosynsis.

Competitive Advantages

• Any form of mutagenesis—Point mutation, deletion or insertion.
• Unlimited sites—We can introduce mutations at any site according to specific needs.
• Highly efficient—Multiple mutation sites can be introduced in one mutagenesis reaction.
• 100% accuracy—Mutant constructs are verified by sequencing analysis.
• One-stop solutions—We provide comprehensive upstream and downstream services for site-directed mutagenesis.
• Competitive price—Unbeatable prices for high-quality services.

CD Biosynsis has been involved in the field of protein design and engineering for many years. We are fully committed to working with you to facilitate the successful completion of the projects. If you are interested in our services, please contact us for more details.

References

1. Drufva E E, et al. Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases. Synthetic and Systems Biotechnology, 2020, 5(2): 62-80.
2. Reikofski J & Tao B Y. Polymerase chain reaction (PCR) techniques for site-directed mutagenesis. Biotechnology advances, 1992, 10(4): 535-547.

Please note that all services are for research use only. Not intended for any clinical use.