Staphylococcus aureus Cas9

Catalog Number: GE0010

Specifications Downloads Related products

Specifications

Catalog Number	Product Size	Concentration	Price
GE0010S	70 pmol	1 μM	Online Inquiry
GE0010L	400 pmol	20 μM	Online Inquiry

Product Information
Description	Staphylococcus aureus Cas9 is expressed as a C-terminal 6×His-tagged fusion in E. coli. It is a programmable DNA endonuclease guided by a sgRNA. Staphylococcus aureus Cas9 can catalyze site-specific cleavage of double-stranded DNA. A ~100 nucleotide gRNA complementary to the target site and a 5′ NNGRRT 3′ (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence are required for targeting.
Applications	Random Mutagenesis

Product Information

Description

Staphylococcus aureus Cas9 is expressed as a C-terminal 6×His-tagged fusion in E. coli. It is a programmable DNA endonuclease guided by a sgRNA. Staphylococcus aureus Cas9 can catalyze site-specific cleavage of double-stranded DNA. A ~100 nucleotide gRNA complementary to the target site and a 5′ NNGRRT 3′ (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence are required for targeting.

Applications

Random Mutagenesis

Properties
Storage Temperature	-20°C
Storage Conditions	• 20 mM Tris-HCl • 300 mM NaCl • 0.1 mM TCEP • 50% Glycerol • pH 7.5 at 25°C
Shipping Conditions	Blue ice.
Heat Activation	65°C for 5 min

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Datasheet

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