Trusted by Leading Research & Pharma Institutions

Strain and Cell Development Services

Accelerate your bioprocess development with comprehensive strain and cell line engineering solutions. From microbial chassis optimization to mammalian cell line development, we deliver high-performance production strains tailored to your needs.

Microbial & Mammalian

High-Yield Strains

GMP-Ready

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Trusted by leading research and pharmaceutical institutions

MIT

Pfizer

Stanford

Roche

Harvard

Novartis

Why Choose Our Development

Multiple host platforms

High-throughput screening

AI-assisted optimization

Scalable production support

Microbial Strains

E. coli, Bacillus, Yeast, Pichia

Mammalian Cells

CHO, HEK293, Hybridoma

Metabolic Engineering

High-yield pathway optimization

Titer

Up to 30 g/L

Small molecules

Service Overview

End-to-End Development Solutions

From gene to production-ready strain, our comprehensive development services accelerate your bioprocess from concept to commercialization.

Microbial Strain Development

Comprehensive engineering services for E. coli, Bacillus, yeast, and other microbial hosts.

E. coli, Bacillus, Lactobacillus
Saccharomyces, Pichia pastoris
CRISPR, random mutagenesis

Mammalian Cell Line Development

High-performance CHO and HEK293 cell line development for recombinant protein production.

CHO-K1, CHO-DG44, GS-null
HEK293 transient and stable
Clonal selection and banking

Metabolic Engineering

Pathway optimization and flux engineering for high-yield bioproduct synthesis.

Pathway design and balancing
Precursor supply optimization
Byproduct reduction

Fermentation Development

Process optimization for scalable fermentation from shake flask to bioreactor.

Media optimization
Process parameter optimization
Scale-up support

Adaptive Laboratory Evolution

Non-rational optimization for stress tolerance through laboratory evolution and genome analysis.

Acid, solvent, temperature tolerance
Whole genome sequencing
Rational validation

Cell Banking & QC

GMP-compliant cell banking and comprehensive quality control services.

MCB, WCB preparation
Identity, purity, stability testing
Regulatory documentation

Our Technology

Advanced Development Platforms

State-of-the-art strain development technologies for optimal production outcomes.

Core Technology

Microbial Strain Engineering

Our microbial platform combines CRISPR-based genome editing with high-throughput screening and systems biology approaches to develop high-performance production strains.

CRISPR Engineering

Precision genome modifications

ALE

Adaptive laboratory evolution

Random Mutagenesis

Error-prone PCR, UV, chemical

Systems Biology

Genome-scale metabolic models

Multiple

Host Options

High-Titer

CHO Platforms

Mammalian Platform

CHO Cell Line Development

Our mammalian platform delivers high-titer, stable CHO cell lines with optimized glycosylation profiles for therapeutic protein production.

GS/ DHFR Selection

Validated selection systems

High-Throughput Screening

Automated clone selection

Glycoengineering

Tailored glycosylation profiles

Metabolic Pathway Engineering

Systems metabolic engineering for optimal flux distribution and high-yield product synthesis.

Pathway design and reconstruction
Flux balance analysis
Precursor and cofactor optimization
Compartment engineering

AI-Assisted Optimization

Machine learning models guide strain optimization for improved productivity and robustness.

Multi-omics data integration
Predictive modeling
Design of experiments
Process optimization

Service Specifications

Technical Specifications

Detailed specifications for our strain and cell development services.

Microbial Platforms

E. coli Titer Range 0.1-30 g/L

Pichia pastoris (K. phaffii) Titer Up to 20 g/L

Bacillus Productivity Secretion-optimized

Yeast Platform S. cerevisiae, P. pastoris

Mammalian Platforms

CHO mAb Titer 3-10 g/L typical

Development Timeline 8-16 weeks

Clonal Assurance Single-cell sorting

Cell Banks MCB + 2x WCB

Engineering Methods

CRISPR-Cas9

TALEN/ZFN

Random Mutagenesis

Adaptive Evolution

Quality Control

Genetic Stability

Extended passage testing

Mycoplasma Testing

PCR and culture methods

Productivity Verification

Multiple bioreactor scales

Service Workflow

Our Development Process

From initial assessment to production-ready strain, our streamlined workflow ensures efficient development.

Consultation

Project assessment and host selection based on your product and process requirements.

Product characterization
Host selection
Strategy planning

Engineering

Strain construction and engineering using advanced genetic tools and screening platforms.

Gene manipulation
Library generation
High-throughput screening

Optimization

Process optimization and scale-up for target titer, yield, and productivity.

Fermentation optimization
Process development
Scale-up studies

Delivery

Cell banking and comprehensive documentation for regulatory compliance.

Cell bank preparation
Monoclonality assurance
QC release testing
Technical documentation

Industry Applications

Wide Range of Applications

Our strain development services support diverse industrial applications.

Therapeutic Proteins

Antibodies, enzymes, hormones, and other recombinant proteins for pharmaceutical applications.

Biochemicals

Organic acids, amino acids, vitamins, and other biochemicals for industrial applications.

Biofuels

Ethanol, butanol, biodiesel precursors, and other biofuel-related compounds.

Biomaterials

Biodegradable plastics, polymers, and other materials from renewable sources.

Plant Natural Products

Flavonoids, terpenoids, alkaloids, and other secondary metabolites.

Enzymes

Industrial enzymes for food, feed, textile, and detergent applications.

Customer Testimonials

Trusted by Industry Leaders

"They delivered an E. coli strain that exceeded our titer targets by 300%. The AI-assisted optimization really made a difference in our development timeline."

Biotech Company

Strain Development Team

"Our CHO cell line development project was completed ahead of schedule with exceptional productivity. The regulatory documentation was thorough and complete."

Pharmaceutical Company

Cell Line Development

"The Pichia engineering team developed a strain with significantly improved protein secretion. The fermentation optimization support was excellent."

Dr. Maria Peters

Roche

Scientific Literature

Supported by Peer-Reviewed Research

Systems Metabolic Engineering of Industrial Microorganisms

Multiple authors. Microorganisms. 2023

Comprehensive review of systems metabolic engineering strategies for industrial strain development.

View Publication

Metabolic Engineering for Lactic Acid Production in S. cerevisiae

PMC9769770. Appl Environ Microbiol. 2022

Combining pathway engineering with adaptive evolution for efficient L-lactic acid production.

View Publication

Engineering Chronological Lifespan in Yeast

Wu Z et al. PNAS. 2025

Novel approach linking cellular lifespan engineering with improved chemical biosynthesis.

View Publication

CHO Cell Line Development Strategies

Review in Biotechnology Advances. 2021

Comprehensive review of technologies for CHO cell line development and protein expression.

View Publication

High-Level Protein Expression in E. coli

Review in Microbial Cell Factories. 2022

Strategies for high-level recombinant protein production in E. coli systems.

View Publication

Metabolic Engineering for Organic Acids

Review in Metabolic Engineering. 2023

Recent advances in metabolic engineering for organic acid production.

View Publication

FAQ

Frequently Asked Questions

Find answers to common questions about our strain development services.

Which host organism should I choose for my product?

Host selection depends on multiple factors including product complexity, post-translational modification requirements, expected titer, and downstream processing considerations. E. coli is ideal for simple proteins and small molecules. Yeast offers eukaryotic processing. CHO cells are preferred for therapeutic proteins requiring complex glycosylation. Our team will help you evaluate options based on your specific requirements.

What titers can you achieve with your strains?

Achieved titers vary by product and host: E. coli (0.1-30 g/L for recombinant proteins), Pichia pastoris (up to 20 g/L), CHO cells (3-10 g/L for monoclonal antibodies). For small molecule production, yields depend on the specific compound and pathway complexity. We establish target titers during project scoping and optimize accordingly.

How long does strain development typically take?

Development timelines vary by complexity: microbial strain development (8-16 weeks for engineered strains, additional 8-12 weeks for process optimization), CHO cell line development (12-20 weeks to clonal cell line). Expedited timelines are available for urgent projects. We provide detailed timelines during project scoping.

Do you offer GMP cell banking services?

Yes, we offer comprehensive cell banking services including GMP Master Cell Bank (MCB) and Working Cell Bank (WCB) preparation. Our banks include full characterization testing (identity, purity, stability, productivity) and regulatory documentation packages for IND/IMPD submissions.

What engineering methods do you use?

We employ multiple engineering approaches including CRISPR-Cas9 for precision genome editing, TALEN/ZFN for specific modifications, random mutagenesis (error-prone PCR, UV, chemical) for broad optimization, and adaptive laboratory evolution (ALE) for stress tolerance improvement. Method selection depends on project requirements.

Can you help with fermentation process development?

Absolutely. We offer comprehensive fermentation development including media optimization, process parameter optimization (pH, temperature, oxygen, feeding strategies), and scale-up support from shake flask to bioreactor. We can provide material at multiple scales for your process validation needs.

What quality control testing do you perform?

Our standard QC package includes genetic stability testing (extended passage studies), mycoplasma testing, sterility testing, identity verification (STR profiling for mammalian cells), productivity validation, and product quality assessment. Additional custom testing can be arranged based on your regulatory requirements.

Do you provide regulatory support documentation?

Yes, we provide comprehensive documentation packages including cell line development history, construction records, characterization data, and quality control results. Our documentation supports IND/IMPD submissions and follows ICH guidelines. We can also assist with regulatory strategy consultation.

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Strain and Cell Development Services

Why Choose Our Development

Microbial Strains

Mammalian Cells

Metabolic Engineering

End-to-End Development Solutions

Microbial Strain Development

Mammalian Cell Line Development

Metabolic Engineering

Fermentation Development

Adaptive Laboratory Evolution

Cell Banking & QC

Ready to Develop Your Production Strain?

Advanced Development Platforms

Microbial Strain Engineering

CRISPR Engineering

ALE

Random Mutagenesis

Systems Biology

CHO Cell Line Development

GS/ DHFR Selection

High-Throughput Screening

Glycoengineering

Metabolic Pathway Engineering

AI-Assisted Optimization

Technical Specifications

Microbial Platforms

Mammalian Platforms

Engineering Methods

Quality Control

Genetic Stability

Mycoplasma Testing

Productivity Verification

Our Development Process

Consultation

Engineering

Optimization

Delivery

Wide Range of Applications

Therapeutic Proteins

Biochemicals

Biofuels

Biomaterials

Plant Natural Products

Enzymes

Trusted by Industry Leaders

Supported by Peer-Reviewed Research

Systems Metabolic Engineering of Industrial Microorganisms

Metabolic Engineering for Lactic Acid Production in S. cerevisiae

Engineering Chronological Lifespan in Yeast

CHO Cell Line Development Strategies

High-Level Protein Expression in E. coli

Metabolic Engineering for Organic Acids

Frequently Asked Questions

Ready to Start Your Project?

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