Strain Engineering for 3-Phenylpropanal

CD Biosynsis is dedicated to designing and optimizing the biosynthetic pathway of 3-phenylpropanol based on the theory of synthetic biology, and continuously improving the performance of microbial cell factories through metabolic engineering to achieve a high yield of 3-phenylpropanol.

Background

3-Phenylpropanol is naturally found in strawberries, tea leaves, and other plants, and is a high-value fragrance with an aromatic flavor. 3-Phenylpropanol has been widely used in medicine, cosmetics, food, and other fields. Currently, the production method of 3-phenylpropanol relies mainly on plant extraction and chemical synthesis. Chemical synthesis generally requires high temperature and pressure and heavy metal catalysts, which are less efficient and more polluting. Meanwhile, synthetic biology is developing rapidly and various microbial systems have been studied in depth. Therefore, the green production of 3-phenylpropanol using microorganisms as cell factories has gradually replaced its plant-derived and chemically synthesized methods.

Figure 1. The 3-phenylpropanol biosynthetic pathways. (Liu Z, et al., 2021)

What We Provide

Based on the synthetic biology platform, we are able to achieve industrial production of 3-phenylpropanol by regulating gene expression, weakening competitive pathways, and regulating carbon flux allocation.

Deliverables

• Efficient cell factory for 3-phenylpropanol production.
• 3-phenylpropanol.

How We Can Help

Development of Microbial Chassis for 3-Phenylpropanol Production

We are able to link 3-phenylpropanol to the microorganism's metabolic network and construct a microbial cell factory for the synthesis of 3-phenylpropanol using renewable resources, enabling sustainable large-scale production of 3-phenylpropanol. The following are the microbial chassis that has been used for the production of 3-phenylpropanol. Please contact us directly if you have other chassis of interest for 3-phenylpropanol production.

Retrobiosynthetic Design of 3-Phenylpropanol Biosynthetic Pathway

We are able to achieve the highest yield of microbial production of 3-phenylpropanol by the computer-aided design of the biosynthetic pathway of 3-phenylpropanol based on a retrobiosynthetic strategy and reconstituting the engineered strain through chromosome engineering.

Screening the Best Pathway for the Production of 3-Phenylpropanol

We are able to analyze and compare the synthetic pathways for the production of 3-phenylpropionic acid. We are able to screen the pathways capable of achieving high yields of 3-phenylpropanoic acid using cheap carbon sources by analyzing the accumulation of the by-product cinnamyl alcohol, the fermentation time of the microorganisms, and the conversion efficiency of the synthetic pathways.

Optimizing Fermentation Conditions

We are able to help our customers select the most appropriate fermentation parameters for cell growth, including temperature, carbon source, etc., to improve fermentation efficiency.

Applications of 3-Phenylpropanol

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive 3-phenylpropanol production and meet the needs of customers in a variety of industries.

• Used for the treatment of cholecystitis and post-biliary surgery syndrome.
• As an edible spice additive.
• Involved in the preparation of coatings and resins.
• Used in cosmetic neighborhoods.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in 3-phenylpropanol production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Liu Z, et al. Metabolic engineering of Escherichia coli for de novo production of 3-phenylpropanol via retrobiosynthesis approach. Microb Cell Fact. 2021 Jun 27; 20(1): 121.

Please note that all services are for research use only. Not intended for any clinical use.