Strain Engineering for Cadaverine

CD Biosynsis is committed to using synthetic biology techniques based on genetic engineering, metabolic engineering, and protein engineering strategies to create environmentally friendly and efficient cadaverine production chassis cells for a wide range of cadaverine applications in multiple fields.

Background

Cadaverine is a physiologically active nitrogenous base widely found in living organisms and is produced by the decarboxylation of lysine in the presence of decarboxylase during protein decay. Cadaverine is an important monomer for a variety of high-value-added products and is widely used in automotive, sporting goods, plastics, electronics, and other fields. Petroleum is the main raw material for cadaverine production. However, the increasing scarcity of petroleum resources and the multiple problems of chemical synthesis of cadaverine make it urgent to develop a method for the efficient production of cadaverine from renewable resources.

Figure 1. Glutarate biosynthesis via lysine catabolism. (Li W, et al., 2019)

What We Provide

Based on a synthetic biology platform, our scientists combine systems biology analysis with genetic engineering, metabolic engineering and protein engineering technologies to achieve the resolution and modification of the cadaverine synthesis pathway for low-carbon and efficient cadaverine biosynthesis.

Deliverables

• Cadaverine producing microorganisms.
• Cadaverine.

How We Can Help

Development of Synthetic Biology Chassis for Cadaverine Production

We are able to achieve cadaverine production using glucose, galactose, starch, mannose, xylose, etc. as carbon sources. The following are the microbial chassis that have been used for the production of cadaveric amines, and we are able to provide modification services for these microbial chassis. Please contact us directly if you have other microbial chassis of interest for cadaverine production.

Whole Cell Transformation for Cadaverine Production

We are able to construct and optimize a whole-cell catalyst for the production of cadaveric amines. We are able to optimize the substrate transport module, product transport module, cofactor transport module, and product synthesis module in the cadaverine production cell factory based on a systematic metabolic engineering strategy to improve the performance of cadaverine production cell engineering.

Optimization of Promoter Element

The appropriate promoter is important for the efficient expression of lysine decarboxylase and the improvement of cadaverine production. We have a library of promoter elements and can help our customers to screen or design and synthesize promoters that are most beneficial for cadaverine synthesis.

Mixed Culture Fermentation

We are able to help our customers build new fermentation systems that take advantage of the synergy between the two engineered bacteria to increase the yield of cadaverine.

Applications of Cadaverine

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive cadaverine production and meet the needs of customers in a variety of industries.

• Regulation of plant senescence as a second messenger.
• Promotes fruit development.
• Used in the synthesis of polyamide nylon.
• Promotes pistil development.
• Used in the treatment of cardiac arrhythmias.
• Used in the treatment of dysentery.
• Improves cold tolerance of plants.
• Used to regulate the concentration of iron ions in microorganisms.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in cadaverine production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

References

1. Li W, et al. Targeting metabolic driving and intermediate influx in lysine catabolism for high-level glutarate production. Nat Commun. 2019 Jul 26; 10(1): 3337.
2. Wang X, et al. Ameliorating end-product inhibition to improve cadaverine production in engineered Escherichia coli and its application in the synthesis of bio-based diisocyanates. Synth Syst Biotechnol. 2021 Sep 14; 6(4): 243-253.

Please note that all services are for research use only. Not intended for any clinical use.