Strain Engineering for cis-Muconic Acid

CD Biosynsis is committed to using synthetic biology to re-engineer the cis-muconic acid synthesis pathway in engineered bacteria or to construct new synthesis pathways to serve our customers.

Advantages of Synthetic Biology-Driven cis-Muconic Acid Production

Muconic acid is a promising C6 platform chemical with excellent UV absorption properties at 260nm, which can be used as a raw material for the production of resins, pharmaceuticals, and pesticides. The production of cis-muconic acid is mainly based on aromatic compounds of petroleum origin. However, petroleum is a non-renewable resource, and its use has a high environmental impact and harsh synthesis conditions. Therefore, synthetic biology-driven production of cis-muconic acid, which constructs a cell factory for cis-muconic acid production, is in line with environmentally friendly principles and has a promising development.

Figure 1. Proposed nonnative pathway for muconic acid synthesis. (Sengupta S, et al., 2015)

What We Provide

We are able to help our customers improve the productivity of cis-muconic acid by using synthetic biology methods, metabolic engineering, and protein engineering to genetically modify the cis-muconic acid heterologous pathway through synthetic promoters, and rational design of enzyme molecules and backbone proteins.

Deliverables

• Efficient cell factory for cis-muconic acid.
• cis-Muconic acid.

How We Can Help

Development of Novel Pathway for cis-Muconic Acid Production

Based on research into the biosynthetic pathway of cis-muconic acid, our scientists can help our customers design unnatural pathways for the production of cis-muconic acid. Cis-muconic acid is produced by the functional expression of unnatural genes in engineered bacteria.

Optimization of cis-Muconic Acid Cell Factories

The biosynthesis of cis-muconic acid is mainly achieved using engineered bacteria-containing plasmids. For the optimization of cis-muconic acid cell factories, we can offer the following specific services.

• We can modify and optimize strains for the production of 3-deoxymanganic acid using rational design principles and genome replication engineering.
• We can use genome replication engineering to bring cells into a state of high mutation rate by introducing a series of genetically modified DNA polymerase correction elements into the microorganism. We provide high throughput screening of engineered bacteria by measuring UV absorbance values.
• We offer two types of fermentation options for the production of cis-muconic acid by microbial fermentation. These two fermentation methods are shaking flask fermentation and fermentor make-up fermentation.

Development of Carbon Source for cis-Muconic Acid Production

Different carbon sources have an important influence on the production of cis-muconic acid. Based on the cell factories we have built, we can help our customers to find safe and inexpensive carbon sources that can be used for large-scale production of cis-muconic acid.

Applications of cis-Muconic Acid

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive cis-Muconic Acid production and meet the needs of customers in a variety of industries.

• Used in the preparation of UV protectants.
• Used in the preparation of military specialties, such as stealth aircraft coatings.
• For the preparation of functional resins.
• As a potential raw material for pharmaceutical chemicals.
• As a potential raw material for agrochemicals.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in cis-muconic acid production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Sengupta S, et al. Metabolic engineering of a novel muconic acid biosynthesis pathway via 4-hydroxybenzoic acid in Escherichia coli. Appl Environ Microbiol. 2015 Dec; 81(23): 8037-43.

Please note that all services are for research use only. Not intended for any clinical use.