Strain Engineering for Glutaric Acid

CD Biosynsis is dedicated to the development and optimization of synthetic pathways for glutaric acid based on synthetic biology platforms, and the search for glutaric acid production technologies with short fermentation cycles, high conversion rates and high yields.

Advantages of Synthetic Biology-Driven Glutaric Acid Production

Glutaric acid is an important C5 platform chemical that serves as a synthetic precursor chemical for a variety of value-added C5 derivatives. Glutaric acid has been widely used in chemical, pharmaceutical, and agricultural applications. Currently, the synthesis of glutaric acid is mainly based on the oxidation of highly aromatic compounds such as cyclohexanol and cyclopentanol, and the nitric acid oxidation of 2-cyanocyclopentanone. The above-mentioned pathways for the synthesis of glutaric acid usually require extreme reaction conditions and produce toxic substances. However, the production of synthetic biologically driven glutaric acid mainly utilizes microbial fermentation and enzyme catalysis. Thus, the production of glutaric acid using synthetic biology techniques can not only be carried out under milder conditions but also be a green and sustainable process.

Figure 1. Multiple engineering strategies for constructing efficient glutaric acid cell factories. (Uranukul B, et al., 2019)

What We Provide

Based on our synthetic biology platform, we are able to help our customers achieve microbiological production of glutaric acid.

Deliverables

• Efficient cell factory for glutaric acid production.
• Glutaric acid.

How We Help

Establishment of Metabolic Pathways for Biosynthesis of Glutaric Acid

The biosynthetic pathways of glutaric acid mainly include α-ketoglutarate reduction, transadipate degradation, α-ketoglutarate carbon chain extension and decarboxylation, 5-aminoglutarate pathway and cadaveric pathway. We are able to use genetic engineering and metabolic engineering tools to modulate the glutaric acid biosynthetic pathway to increase glutaric acid production. The specific services we are able to provide are as follows and include but are not limited to.

• We are able to construct recombinant strains overexpressing the 5-aminoglutarate pathway of DavB, DavA, GabT and GabD for glutaric acid production.
• We are able to construct recombinant strains that regulate the expression of key genes under the control of a synthetic promoter.
• We are able to construct recombinant strains containing codon-optimized genes.
• We are able to design a multi-enzyme cascade pathway and reconstitute the glutaric acid production pathway in vitro.

Optimization of Whole Cell Reaction Condition

Substrates, intermediate metabolites, by-products, products, temperature, pH, etc. can all affect the production of glutaric acid. We are able to help our customers establish optimal whole-cell catalytic systems.

Applications of Glutaric Acid

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive glutaric acid production and meet the needs of customers in a variety of industries.

• Used to make synthetic rubber.
• Used to make synthetic resins.
• Used to make nylon.
• Used to make plasticizers.
• As a precursor chemical for the synthesis of 1,5-pentanediol.
• As a precursor chemical for the synthesis of 5-hydroxyvaleric acid.

Want to Learn More?

As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for bio-based chemical production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Wang J, et al. Engineering the Cad pathway in Escherichia coli to produce glutarate from L-lysine. Appl Microbiol Biotechnol. 2021 May; 105(9): 3587-3599.  

Please note that all services are for research use only. Not intended for any clinical use.