Strain Engineering for Isoprene

CD Biosynsis is committed to helping customers develop green and high-yield isoprene biosynthesis technologies by modifying the metabolic pathways of strains through genetic engineering and metabolic engineering.

Advantages of Synthetic Biology-Driven Isoprene Production

Isoprene is an important C5 platform chemical with a wide range of applications in rubber, food, fragrances, and aviation fuels. The synthesis of isoprene is mainly divided into chemical synthesis and biosynthesis. Although chemical synthesis and technology are improving, with the gradual depletion and non-renewability of oil, the material shortage will become a bottleneck for the industrial production of isoprene. Certain plants and microorganisms are capable of producing isoprene. However, the production of isoprene by plants and microorganisms under natural conditions is less productive and difficult to collect and is susceptible to climate and growth cycles. Therefore, large-scale production of isoprene from plants is not practical. The ability to obtain monomeric isoprene using microbial fermentation, which has the advantages of low cost, high yield, short production cycle, and low pollution, has become a hot research topic in this field in recent years.

Figure 1. Isoprene biosynthesis pathway divided into three modules using key intermediates as the connecting nodes. (Ye L, et al., 2016)

What We Provide

There is a wide variety of wild-type strains that synthesize isoprene, but the yield of isoprene is low and does not meet the production demand. Therefore, our scientists use genetic and metabolic engineering based on a synthetic biology platform to help our customers achieve large-scale production of isoprene.

Deliverables

• Efficient cell factory for isoprene production.
• Isoprene.

How We Can Help

Development of Synthetic Biology Chassis for Isoprene Production

Microorganisms have the advantages of small size, fast growth, and the ability to utilize renewable substrate resources. Therefore, the use of microbial fermentation to produce isoprene is of great importance. We have successfully used the following microorganisms to produce monomeric isoprene, and if you wish to synthesize isoprene by other microbial chassis, please contact us directly.

Construction Efficient Isoprene-Production Strains

• We are able to help our customers obtain strains with high isoprene production by using genetic and metabolic engineering to increase the activity of key enzymes and cofactors involved in isoprene synthesis.
• We are able to synthesize key enzymes in the isoprene pathway through gene knockout or mutation techniques to regulate the flow of metabolic pathways and help our customers obtain strains with high isoprene production.
• We are able to construct a multi-gene co-expression system to improve the accumulation of metabolites and increase the final yield of isoprene by regulating the sequence of gene expression.

Construction of Cell-Free Multienzyme Systems

We are able to reconstruct the complete isoprene synthesis pathway in vitro using protein redesign techniques.

Optimization of Fermentation Conditions

We can assist our customers in screening the most suitable carbon, nitrogen, and organic acids for the isoprene fermentation process and help them optimize the fermentation process and fermentation conditions to improve the yield of isoprene.

Applications of Isoprene

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive isoprene production and meet the needs of customers in a variety of industries.

• Used in the manufacture of synthetic rubber and synthetic resin.
• Used in the manufacture of pesticides.
• Used in the manufacture of fragrances.
• Used in the manufacture of lubricant additives.
• Used in the manufacture of pharmaceutical intermediates.
• Used in the manufacture of catalysts.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in isoprene production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Ye L, et al. Engineering microbes for isoprene production. Metab Eng. 2016 Nov; 38: 125-138.

Please note that all services are for research use only. Not intended for any clinical use.