Strain Engineering for Phenol

CD Biosynsis is committed to developing more productive methods of phenol biosynthesis using glycerol, glucose, etc. as carbon sources to provide customers with high-quality phenol products and their derivatives.

Advantages of Synthetic Biology-Driven Phenol Production

Phenol is a basic chemical raw material, mainly used in the production of certain resins, adipic acid, etc. Derivatives of phenol such as nitro phenols and alkyl phenols can be used in the production of pharmaceuticals, fuels, fragrances, and various additives. Currently, there are seven main chemical synthesis methods for phenol. However, the chemical synthesis methods of phenol suffer from a series of disadvantages such as high production costs, high environmental pollution, and more by-products. The biosynthesis methods of phenol mainly use glycerol and glucose as carbon sources. Therefore, biosynthesis of phenol is a more promising method for phenol production that is inexpensive and environmentally friendly.

Figure 1. The pathways for phenol biosynthesis via 4HB and TYR. (Guo X, et al., 2019)

What We Provide

We have built a microbial fermentation platform and developed high-quality microbial chassis for phenol production using molecular biology and fermentation process optimization techniques based on principles such as metabolic engineering to help our customers improve the yield of phenol biosynthesis.

Deliverables

• Efficient cell factory for phenol.
• Phenol.

How We Can Help

Development of Glycerol-Based Biosynthesis of Phenol

Glycerol is an extremely promising carbon source for microbial fermentation. Our scientists are developing phenol biosynthesis services using glycerol as a carbon source and E. coli as a biological chassis by introducing exogenous genes in E. coli. If you would like to develop phenol synthesis using glycerol as a carbon source in other microbial chassis, please fill out the online application form or call us to let us know your requirements.

Development of Glucose-Based Biosynthesis of Phenol

E. coli uses the shikimic acid pathway as a basis for phenol production, using glucose as a carbon source. Our scientists have been working on the development and optimization of glucose-based biosynthesis of phenol in E. coli. If you would like to develop phenol synthesis using glucose as a carbon source in other microbial chassis, please fill out the online application form or call us to let us know your requirements.

Constructed Multi-Enzyme Systems for the Production of Phenol

The biosynthesis of phenol depends on the catalysis of a series of enzymes in microorganisms. Our scientists have extensive experience in establishing artificial biotransformation systems. We can achieve phenol synthesis by constructing multi-enzyme systems, and we offer optimization services for multi-enzyme systems for co-phenol production. Our optimization services for multi-enzyme systems are as follows.

• We use cofactor regeneration technology to increase enzyme activity and thus reduce the cost of phenol production.
• We mixed multiple recombinant bacteria and used some of them to complete the regeneration of cofactors and others to complete the synthesis of target products.

Optimization of Fermentation Conditions

We provide optimization services for the whole process of fermentation parameters in the fermentation of phenol. By optimizing the fermentation parameters, we can help our customers to develop the maximum potential of phenol production.

Applications of Phenol

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive phenol production and meet the needs of customers in a variety of industries.

• Used in the preparation of salicylic acid.
• Used in the preparation of caprolactam.
• Used in the preparation of phenolic resins.
• Phenol derivatives are used in the preparation of pharmaceutical products, dyes, fragrances, and additives.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in phenol production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Guo X, et al. De novo phenol bioproduction from glucose using biosensor-assisted microbial coculture engineering. Biotechnol Bioeng. 2019 Dec; 116(12): 3349-3359.

Please note that all services are for research use only. Not intended for any clinical use.