Strain Engineering for Polyhydroxyalkanoate

CD Biosynsis works on the optimization of microbial strains, carbon substrates, nutrients and fermentation conditions in the biosynthesis of polyhydroxyalkanoate to improve its biosynthesis efficiency.

Advantages of Synthetic Biology-Driven Polyhydroxyalkanoate Production

Polyhydroxyalkanoate is an industrially important biomolecule and a potential alternative to petrochemical plastics. Of great interest due to their bio-based, biodegradable, biocompatible, and non-toxic properties, polyhydroxyalkanoates are produced by a variety of microbial species and often accumulate as carbon reserves in the presence of nutrient imbalances. Although polyhydroxyalkanoates have promising environmental benefits, their industrial production is limited by high production costs. The synthesis of polyhydroxyalkanoate based on a microbial fermentation process using waste as a substrate using synthetic biology techniques would greatly reduce its production cost.

Figure 1. Next generation industrial biotechnology (NGIB) based on engineered extremophilic bacteria. (Zheng Y, et al., 2020)

What We Provide

Deliverable

• Polyhydroxyalkanoate.
• Value-added products accompanying polyhydroxyalkanoate synthesis.

How We Can Help

Microbial Fermentation for Polyhydroxyalkanoate Production

The biosynthesis of polyhydroxyalkanoate relies on biological processes involving the intracellular formation of polyhydroxyalkanoate from microbial cultures as a carbon and energy store. The production of polyhydroxyalkanoate follows the following four stages, the growth of bacteria and accumulation of polyhydroxyalkanoate, isolation of the culture from the liquid, recovery of polyhydroxyalkanoate from the biomass and washing and drying of the isolated polyhydroxyalkanoate. We are able to produce polyhydroxyalkanoate using the following microorganisms.

Morphology Engineering for Polyhydroxyalkanoate Production

The separation of polyhydroxyalkanoate is influenced by cell size. The large cell size contributes to the separation of bacterial cells and the accumulation of polyhydroxyalkanoate particles. We are able to regulate the expression of genes related to the maintenance of bacterial cell morphology to alter cell size and thus enhance polyhydroxyalkanoate accumulation.

Improving Polyhydroxyalkanoate Production

The diversity of microbial metabolism determines different routes for the synthesis of polyhydroxyalkanoate, and changes in substrates can also lead to differences in their synthetic routes. We provide optimization services for the main routes of polyhydroxyalkanoate synthesis from different substrates by different microorganisms. The specific optimization services involve the following synthetic pathways.

• Ralstonia eutropha and most bacteria synthesize PHB from sugar.
• Rhodospirillum rubrum synthesize PHB from sugar.
• Pseudomonas oleovorans and other bacteria synthesize PHAs from medium-chain alkanes, alcohols, and acids.
• Pseudomonas aeruginosa and other bacteria synthesize PHAs from sugar and carbon sources.
• Ralstonia eutropha synthesis of PHBV from sugar and propionic acid.

Co-Synthesis of Polyhydroxyalkanoate with Value-Added Products

Using an integrated approach to obtain biologics with multiple economic benefits is one way to reduce the cost of polyhydroxyalkanoate production. The value-added products that accompany polyhydroxyalkanoate synthesis are polysaccharides, microbial proteins, carotenoids, etc. We are committed to developing bio-refined methods for the simultaneous production of polyhydroxyalkanoate and value-added products and providing integrated production services for bioproducts.

Applications of Polyhydroxyalkanoate

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive polyhydroxyalkanoate production and meet the needs of customers in a variety of industries.

• For the preparation of biomedical implants.
• For tissue engineering.
• For the preparation of biocomposites.
• For the manufacture of nutritional supplements.
• For the preparation of drug and particle carriers.
• For the preparation of packaging materials.
• For the manufacture of textiles.
• For the preparation of biofuels.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in polyhydroxyalkanoate production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Zheng Y, et al. Engineering biosynthesis of polyhydroxyalkanoates (PHA) for diversity and cost reduction. Metab Eng. 2020 Mar; 58: 82-93.

Please note that all services are for research use only. Not intended for any clinical use.