Strain Engineering for Valine

CD Biosynsis is dedicated to solving problems in valine biosynthesis and improving the production efficiency and conversion rate of valine so as to achieve large-scale production of valine.

Background

Valine is one of the three branched-chain amino acids and an essential amino acid for human beings. Valine has various physiological functions and plays an irreplaceable role in various physiological activities of living organisms. Valine is widely used in medicine, food additives, feed additives, and other fields. Currently, valine is produced by extraction, synthesis, and fermentation methods. However, the extraction method and chemical synthesis method are costly and the reaction is complicated, which is not suitable for industrial production. Therefore, it is important to develop synthetic biology methods for the large-scale production of valine.

Figure 1. Engineering strategies for enhancing L-valine production in model organisms as microbial cell factories. (Gao H, et al., 2021)

What We Provide

Based on synthetic biology strategies, we are able to optimize the microbial production process of valine and maximize the potential of the valine biosynthesis pathway.

Deliverables

• Efficient cell factory for valine production.
• Valine.

How We Can Help

Development of Microbial Chassis for Valine Production

We can help our customers develop microbial strains for the efficient production of valine. Our scientists are able to select high-yielding mutant strains based on biosynthetic pathways. We have achieved the synthesis of valine with the strains in the following table. Please contact us directly if you have other chassis of interest for valine production.

Optimization of Fermentation Conditions

Oxygen supply, different carbon and nitrogen sources, and biotin concentration can affect the yield of valine. We can ensure a significant increase in microbial growth and fermentation product yield by optimizing the fermentation conditions parameters.

Selection and Breeding of Valine High-Yielding Bacteria

We can select and breed leucine and isoleucine nutrient-deficient mutant strains or anti-feedback-regulated mutant strains to increase valine production by changing the metabolic pathway and increasing precursor substance synthesis.

Applications of L-Valine

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive valine production and meet the needs of customers in a variety of industries.

• As a feed additive.
• As a flavoring agent.
• For the treatment of hypertension.
• Used to improve the flavor of bread.
• As an athlete's drink and amino acid energy drink.
• Used to relieve muscle fatigue.
• Used in the manufacture of amino acid injections.
• Used in the synthesis of anti-tumor APIs.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in valine production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

1. Gao H, et al. Engineering of microbial cells for L-valine production: challenges and opportunities. Microb Cell Fact. 2021 Aug 30; 20(1): 172.

Please note that all services are for research use only. Not intended for any clinical use.