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Succinate Dehydrogenase (SDH) Activity Assay Kit

Catalog Number: AKAS0535

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Specifications

Product Information
Product Name Succinate Dehydrogenase (SDH) Activity Assay Kit
Detection Method Colorimetric method
Instruments Microplate Reader
Sample Type Animal Tissue, Cell
Detection Principle SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP). Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm.
Assay Time 10 min
Other Reagents Normal saline (0.9% NaCl), PBS(0.01 M,pH 7.4).
Storage -20°C

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