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Superoxide Anionic Assay Kit - Colorimetric (WST-1 Method)

Catalog Number: AKAS0539

Price: Online Inquiry

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Specifications

Product Information
Product Name Superoxide Anionic Assay Kit - Colorimetric (WST-1 Method)
Instruments Microplate Reader
Sample Type Serum, Plasma, Urine, Cells, Cell Culture Supernatant, Leucocyte
Research Area Oxidative Stress Reactive Oxygen Species
Detection Principle Superoxide anion free radicals are produced through the reaction system of xanthine and xanthine oxidase. WST-1 (a water-soluble tetrazolium salt) can react with the generated superoxide anion to produce water-soluble formazan. When the tested sample contains the superoxide anion free radical inhibitor, it can inhibit the formation of formazan. When the tested sample contains the substance that produces superoxide anion free radical, it can promote the formation of formazan dye. By colorimetric analysis of WST-1 products, the units of activity of inhibition or production of superoxide anion radical in samples can be calculated.
Assay Time 35 min
Storage Reagent 3:-20°C;, Others:2-8°C
Shipping Conditions Gel Pack

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