Synthetic DNA Library Construction

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CD Biosynsis provides high-quality synthetic DNA library construction services for synthetic biology researchers around the world to facilitate their cutting-edge research. Our strong expertise coupled with advanced techniques can help bring diverse synthetic applications and creative ideas to fruition.

Introduction to Synthetic DNA Library

Synthetic DNA library refers to a collection of DNA fragments stored in host microorganisms by molecular cloning. Each fragment can be stored in a host organism separately. By rapidly and accurately replicating DNA in host organisms and producing detectable quantities, we can have quick access to many different sequences of interest with ease and efficiency. Synthetic DNA libraries enable us to achieve gene synthesis and cloning faster, more intelligent, and more reliable.

Synthetic DNA library provides a more accurate and effective approach for understanding and studying genetics. The design and construction of DNA libraries play an important role in identifying novel gene regulatory sequences or the minimal domains that are critical to protein structure and functions. Synthetic DNA libraries can also be used to construct genetic circuits of biosynthesis pathways for metabolic engineering.

Figure 1. Cloned libraries – RNAi and peptide libraries. (Murgha Y E., 2012)

Applications of Synthetic DNA Library

Gene expression.
Enzyme engineering
Synthetic biology
Discovery biology

Protein engineering
Antibody development
Structural biology
Directed evolution

Service Offering

CD Biosynsis offers a wide range of library types specific to the customer’s specifications. If you cannot find the library you need in the list below, please don't hesitate to contact us.

Library Type	Description	Application
Site Saturation Library	Substitution of all 20 possible amino acids at any site.	Structural-functional analysis. Generation of variants for directed evolution.
Random Substitution Library	Substitution of bases with random, triplet, or degenerate ones.	Identify locations for specific analysis. Determine beneficial substitutions in protein engineering.
Modular Substitution Library	Build new genetic modules or pathways from multiple DNA elements.	Genetic circuit engineering. Pathway engineering.
Alanine Scan Library	Sequentially replace an individual amino acid with alanine at every position.	Research on amino acid residues critical to protein function, structure, and interaction.
Antibody Library	Establish high diversity synthetic antibody libraries.	Improve specificity, immunogenicity, affinity, and other existing functionality.
Combinatorial Library	Use different genetic elements in multiple combinations.	Determine the most beneficial combination.
Truncation library	Systematically truncate amino acids from each terminus or both.	Identify the shortest amino acid sequence for protein function.

Competitive Advantages

Powerful one-stop solutions for synthetic DNA library construction.
Customized services based on a wide range of library types.
High quality and reliable services coupled with best-in-class consultation.
Fast turnaround time and competitive price.

CD Biosynsis offers high-quality synthetic DNA libraries of any size and complexity to facilitate various synthetic biology applications. If you are interested in our services or have any specific needs, please feel free to contact us for more details.

References

Murgha Y E. Large-Scale Generation of Synthetic DNA Libraries: Sequence-Specific Priming of Reverse Transcription. 2012.
Acevedo-Rocha C G & Reetz M T. Assembly of Designed Oligonucleotides: a useful tool in synthetic biology for creating high-quality combinatorial DNA libraries. Directed Evolution Library Creation. Springer, New York, NY, 2014: 189-206.

Please note that all services are for research use only. Not intended for any clinical use.

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