TALDO1 Knockout cell line （HT-29）

The TALDO1 Knockout cell line (HT-29) is primarily utilized in cancer research and metabolic studies providing insights into tumor metabolism and cellular signaling pathways This cell line enables researchers to investigate the role of transaldolase 1 (TALDO1) in colorectal cancer progression as well as its contribution to nucleotide and pentose phosphate pathway alterations

In specific experimental contexts such as drug response assays and metabolic profiling scientists can utilize this knockout model to compare cellular responses with wild-type cell lines thereby elucidating the impact of TALDO1 deficiency This integration into workflows involves routine culture and transfection techniques along with analyses such as Western blotting and flow cytometry to confirm gene knockout efficiency and functional consequences

The practical benefits of using the TALDO1 Knockout line include the ability to dissect metabolic dependencies of cancer cells facilitating the identification of potential therapeutic targets Furthermore this cell line is particularly valuable in metabolomics studies where the absence of TALDO1 can reveal compensatory metabolic pathways enhancing our understanding of cancer metabolism and targeting strategies