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Total Cholesterol and Cholesteryl Ester Assay Kit - Fluorometric

Catalog Number: AKAS0561

Price: Online Inquiry

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Specifications

Product Information
Product Name Total Cholesterol and Cholesteryl Ester Assay Kit - Fluorometric
Instruments Microplate Reader
Sample Type Serum, Plasma, Animal Tissue, Cells
Research Area Ferroptosis Diabetes & Obesity
Detection Principle Total cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.
Assay Time 20 min
Storage -20°C
Shipping Conditions Gel Pack

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