Total Cholesterol and Cholesteryl Ester Assay Kit - Fluorometric
Catalog Number: AKAS0561
Price: Online Inquiry
Catalog Number: AKAS0561
Price: Online Inquiry
Product Information | |
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Product Name | Total Cholesterol and Cholesteryl Ester Assay Kit - Fluorometric |
Instruments | Microplate Reader |
Sample Type | Serum, Plasma, Animal Tissue, Cells |
Research Area | Ferroptosis Diabetes & Obesity |
Detection Principle | Total cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE). Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration. |
Assay Time | 20 min |
Storage | -20°C |
Shipping Conditions | Gel Pack |
Please note that all services are for research use only. Not intended for any clinical use.
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