Total Cholesterol Assay Kit - Colorimetric
Catalog Number: AKAS0550
Price: Online Inquiry
Catalog Number: AKAS0550
Price: Online Inquiry
Product Information | |
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Product Name | Total Cholesterol Assay Kit - Colorimetric |
Instruments | Microplate Reader |
Sample Type | Serum, Plasma, Tissue, Cells, Cell Culture Supernatant |
Research Area | Diabetes & Obesity |
Detection Principle | Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated. |
Assay Time | 20 min |
Storage | 2-8°C |
Shipping Conditions | Gel Pack |
Please note that all services are for research use only. Not intended for any clinical use.
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