Tth Argonaute

Catalog Number: GE0016

Specifications Downloads Related products

Specifications

Catalog Number	Product Size	Concentration	Price
GE0016	50 pmol	1 μM	Online Inquiry

Product Information
Description	Thermus thermophilus argonaute (TrAgo) is a prokaryotic programmable DNA-endonuclease from the Gram-negative, thermophilic bacterium Thermus thermophilus. As a DNA-guided endonuclease, it requires a 16-18 nucleotide long 5’-phosphorylated single-stranded DNA oligonucleotide guide to target its activity to a specific corresponding sequence on a substrate. TrAgo is active from 65-85°C. It exihibits mild activity on single-stranded RNA, moderate to high activity on double-stranded DNA, and high activity on single-stranded DNA.
Applications	Single-tube, isothermal generation of the Cas9-sgRNA constructs.

Product Information

Description

Thermus thermophilus argonaute (TrAgo) is a prokaryotic programmable DNA-endonuclease from the Gram-negative, thermophilic bacterium Thermus thermophilus. As a DNA-guided endonuclease, it requires a 16-18 nucleotide long 5’-phosphorylated single-stranded DNA oligonucleotide guide to target its activity to a specific corresponding sequence on a substrate.
TrAgo is active from 65-85°C. It exihibits mild activity on single-stranded RNA, moderate to high activity on double-stranded DNA, and high activity on single-stranded DNA.

Applications

Single-tube, isothermal generation of the Cas9-sgRNA constructs.

Properties
Storage Temperature	-20°C
Storage Conditions	• 10 mM Tris-HCl • 300 mM NaCl • 1 mM DTT • 0.1 mM EDTA • 50% Glycerol • pH 7.4 at 25°C
Shipping Conditions	Blue ice.

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Datasheet

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