TUNEL 1-Step In Situ Apoptosis Kit

Catalog Number: AKAS0578

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Specifications

Product Information
Product Name	TUNEL 1-Step In Situ Apoptosis Kit
Detection Method	Fluorometric Method
Sample Type	Paraffin Section, Frozen Section, Cell Slide
Research Area	Apoptosis TUNEL 1-Step Kits
ASSAY SIZE	20T/50T/100T/
Detection Principle	When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.
Applications	DNA Fragmentation
Assay Time	3 hours
Dye Type	FITC
Ex/EM	490/520
Channel Set	FITC
Other Reagents	4% Paraformaldehyde, 0.2% Triton X-100, PBS, ddH2O, Mounting solution with anti-fluorescence quencher
Storage	RT/-20°C. Refer to the Manual.
Shipping Conditions	Ice pack

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