Whole-Cell Biosynthesis

Whole-Cell Biosynthesis in Synthetic Biology

Whole-cell biosynthesis is a rapidly growing field in synthetic biology that involves the use of living cells as a platform for the production of valuable compounds. This approach offers a number of advantages over traditional chemical synthesis, including improved efficiency, reduced waste, and the ability to use renewable resources. CD Biosynsis is a leading company in the field of whole-cell biosynthesis, providing a range of services for the production of a wide variety of compounds using genetically modified microorganisms.

Figure 1: A whole-cell biocatalytic strategy was developed to acylate esculin, and the introduction of medium- and long-length fatty acids chains in acylation endowed esculin with non-selective cytotoxicity. (Hao L.S., et al. 2021)

Our Services for Whole-Cell Biosynthesis

At CD Biosynsis, we offer a range of services for whole-cell biosynthesis, including strain development, fermentation optimization, and downstream processing. Our team of experts works closely with each client to develop a customized solution that meets their specific needs.

Strain Development

Our strain development service is designed to help clients engineer microorganisms to produce specific compounds. Our team of experts uses a variety of genetic engineering techniques to optimize the production of target compounds, including gene knockout, knock-in, and overexpression. We can also engineer microorganisms to produce novel compounds with desired properties. Our strain development services include:

• Gene knockout: We use CRISPR-Cas9 or other gene-editing tools to delete genes that may interfere with the production of target compounds, resulting in improved yields and purity.
• Knock-in: We insert genes encoding enzymes that are required for the biosynthesis of specific compounds, allowing for their production by the host organism.
• Overexpression: We can engineer microorganisms to overexpress genes that are involved in the biosynthesis of specific compounds, resulting in increased production.

Fermentation Optimization

Our fermentation optimization service is designed to maximize the production of target compounds by optimizing the growth and metabolism of microorganisms. We use a variety of techniques to optimize fermentation conditions, including:

• Media optimization: We develop customized media formulations based on the specific needs of each microorganism, ensuring optimal growth and metabolism.
• Process optimization: We optimize key fermentation parameters such as temperature, pH, and agitation to maximize the production of target compounds.
• Scale-up: We can scale up fermentation processes from small-scale lab experiments to large-scale industrial production, ensuring consistent production of high-quality compounds.

Downstream Processing

• Chromatography: We use various types of chromatography, such as ion exchange, size exclusion, and affinity chromatography, to separate and purify target compounds from other components in the fermentation broth.
• Crystallization: We use crystallization techniques to purify target compounds to a high degree of purity.
• Other purification methods: We also use other purification methods, such as solvent extraction and precipitation, as necessary.

Our Advantages

• Efficient Production
  • High yields of target compounds.
  • Reduced waste and environmental impact.
  • Use of renewable resources.
• Customized Solutions
  • Tailored strain development services.
  • Customized fermentation optimization.
  • Downstream processing to meet specific purity requirements.
• Advanced Technology
  • Use of CRISPR-Cas9 and other gene-editing tools for strain development.
  • Advanced fermentation techniques for optimal growth and metabolism.
  • Various purification methods for downstream processing.

The services for whole-cell biosynthesis at CD Biosynsis are designed to help clients produce valuable compounds using genetically modified microorganisms. Our approach is based on advanced genetic engineering techniques, fermentation optimization, and downstream processing, resulting in improved efficiency, reduced waste, and the use of renewable resources. Contact us today to learn more about our customized solutions for strain development, fermentation optimization, and downstream processing, and how we can help you meet your research and development needs.

References

1. Hao L.S., et al. Highly efficient whole-cell biosynthesis and cytotoxicity of esculin esters. Journal of Biotechnology, 2021, 337: 46-56.
2. Xu, Y., et al. Engineering of a UDP-Glycosyltransferase for the Efficient Whole-Cell Biosynthesis of Siamenoside I in Escherichia coli. Journal of agricultural and food chemistry, 2022, 70, 5: 1601-1609.

Please note that all services are for research use only. Not intended for any clinical use.