Engineering of Bacterial Chassis for Biosynthesis

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CD Biosynsis has the ability to develop successful strategies for engineering and modifying bacterial chassis to help our customers drive synthetic biology downstream applications. We have developed optimized Design-Build-Test-Learn (DBTL) pipelines to integrate the advantages of model and non-model bacteria. Our scientists will work closely with our customers to exploit the full synthetic biology potential of bacteria.

Introduction to Bacterial Chassis

Engineering of Bacterial Chassis for Synthetic Biology

Synthetic biology focuses on the introduction of new functions in chassis organisms that they do not have in their natural environment. Since bacteria have evolved many useful physiological and metabolic properties, they have become the ideal synthetic biology chassis. In the past few years, there has been an unprecedented increase in the number of novel bacterial species that hole the synthetic biology potential. With the development and application of modern biotechnology such as omics and genome engineering, researchers are no longer limited to using bacterial strains isolated from nature, but have achieved the construction of cell factories based on bacterial chassis. The bacterial chassis have been used not only to unravel biological mechanisms, but also to expand applications in a variety of industries, such as medicine, food, materials, agriculture, and energy.

What We Can Do

CD Biosynsis is committed to providing our customers with ideal bacterial chassis with desirable properties using top-down and bottom-up strategies. Our experts in bacteria synthetic biology have been working to develop highly efficient strategies for identifying essential and non-essential genes in bacteria, large-scale genome reduction, high-throughput genetic engineering, DNA synthesis and assembly, and automated computational design.

Top-down and bottom-up strategies for engineering bacterial chassis – CD Biosynsis.

Applications of Bacterial Chassis

CD Biosynsis applies our considerable experience in synthetic biology to select bacterial species that can be used as chassis to meet expected applications. We have established a library of optimized bacterial chassis covering model and non-model bacteria to guide cell factory design and construction for fundamental research and diverse applications in the biotechnology, pharmacy, and many other fields. We are also working to explore the application potential of extremophilic bacteria such as thermophilic and cryophile bacteria. The following table lists some of the bacterial chassis in our synthetic biology toolbox, which will be updated continuously. Our scientists can help customers construct bacterial chassis using the common developed strains or support them to develop the potential of new strains.

Bacterial Strains	Characteristics	Examples of Applications
Escherichia coli	The best‐studied example among bacteria. A wide range of tools for genetic engineering.	Production of chemicals (e.g., butanol, 1,3-propanediol, polyhydroxyalkanoates (PHAs), and fatty acids).
Bacillus subtilis	Strong capacity for protein production. Numerous genetic tools are available.	Production of recombinant proteins and enzymes (e.g., α‐amylases, β‐glucanases, and laccases).
Corynebacterium glutamicum	Capable of producing high amounts of glutamate. Efficient and comprehensive tools for genetic editing.	Production of amino acids (e.g., glutamate and lysine).
Pseudomonas putida	Robust background metabolism. Remarkable tolerance to chemical stresses.	Production of fine chemicals (e.g., chiral amines, 2-quinoxalinecarboxylic acid, rhamnolipids, terpenoids, and polyketides).
Vibrio natriegens	Extremely short doubling time. Non-pathogenic to humans.	Production of natural products (e.g., alanine, indole-3-acetic acid, and β-carotene).
Zymomonas mobilis	Ability to produce ethanol with an outstanding yield. Ability to use the Entner-Doudoroff (ED) pathway under anaerobic conditions.	Production of industrially relevant compounds.
Acinetobacter baylyi ADP1	High natural transformation efficiency. Highly efficient systems for chromosomal incorporation of exogenous DNA.	Production of tetrapyrrole compounds, triacylglycerol, etc.
Clostridium spp.	Capable of using virtually all simple and complex carbohydrates. Capable of producing a broad spectrum of metabolites.	Produce high-value chemicals and biofuels (e.g., isopropanol, ethanol, and 3-hydroxybutyrate (3-HB)).
Lactic Acid Bacteria	A variety of genome editing techniques have been successfully established. A considerable number of promoters and terminators have already been isolated	Production of green chemicals, fuels, and enzymes (e.g., ethanol, bacteriocins, and proteolytic enzymes).
Cupriavidus necator	Capable of utilizing a wide range of substrates. Capable of fixing CO₂ through Calvin–Benson–Bassham (CBB) cycle.	Production of value-added biochemicals (e.g., PHA, isopropanol, and acetoin).
Halomonas spp.	Able to grow under a high salt concentration. Able to grow under open and continuous fermentation conditions.	Production of diverse PHA (e.g., PHB, P34HB, and PHBV).
Roseobacter clade	Able to survive in aerobic and anaerobic environments. High abundance and availability of physiologically diverse.	Production of secondary metabolites (e.g., antibiotics).
Cyanobacteria	Easily be genetically manipulated and a relatively fast growth. Ability to carry out oxygenic photosynthesis.	Production of renewably-produced fuels and specialty chemicals (e.g., long-chain alcohols, ethylene, alkanes, hydrogen, and ethanol).
Actinomycetes	The major source of biologically active secondary metabolites. Ability to produce novel natural products.	Production of natural products (e.g., tetracyclines, β-lactams, aminoglycosides, and macrolides).

Want to Learn More?

CD Biosynsis has been continuously expanding our synthetic biology toolbox and keeping our knowledge and skills current. We provide full support for our customers' innovations in synthetic biology. If you require any further details, please feel free to contact us and let us know how we can support your new idea or project.

References

Calero P & Nikel P I. Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms. Microbial Biotechnology, 2019, 12(1): 98-124.
Borkowski O, et al. Overloaded and stressed: whole-cell considerations for bacterial synthetic biology. Current opinion in microbiology, 2016, 33: 123-130.

Please note that all services are for research use only. Not intended for any clinical use.

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