Strain Engineering for Fumaric Acid

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CD Biosynsis is dedicated to improving the productivity of fumaric acid in cellular systems through genetic engineering, metabolic engineering, and enzyme engineering techniques, and to establishing optimal parameters for biosynthesis through precision fermentation techniques to provide ideas for improving microbial production of organic acids.

Background

Fumaric acid is an important platform chemical that can be easily esterified and polymerized due to its multifunctional structure, making it a raw material for industrial products such as resins, unsaturated polyester resins, and alkyd resins. Fumaric acid has been used in the production and processing of food, beverages, detergents, animal feed, pharmaceuticals, and various industrial products. Currently, the petrochemical production route of fumaric acid uses hydrocarbons as raw materials, and carbon monoxide and carbon dioxide are formed during the production process, causing harm to the environment. With the depletion of crude oil, the increasing demand for fumaric acid, and the emphasis on sustainability, synthetic biology-driven fumaric acid generation has become a focus of research.

Figure 1. The metabolic pathway for fumaric acid production. (Xu Q, et al., 2012)

What We Provide

Based on a synthetic biology platform, we are working to improve the fermentation process of fumaric acid using renewable raw materials and genetically modified microorganisms. The following are the platforms and tools involved in providing fumaric acid biosynthesis services. Included but not limited to.

Featured Services

We are able to help our customers increase the yield of fumaric acid using gene editing tools such as CRISPR/Cas-9 and RNA interference.
We can obtain a large number of new enzymes through protein sequence and structure databases, and design appropriate biocatalysts with bioinformatics tools.
Based on our metabolomics platform, we use metabolomics analysis to help our customers further understand the factors that influence fumaric acid biosynthesis and develop guidance strategies that can be successfully applied to culture media optimization.
We use different fermentation parameters to help customers explore the best conditions for biosynthesis.

Key Technologies

Deliverables

Efficient cell factory for fumaric acid production.
Fumaric acid.

How We Can Help

Microbial Strain Improvement

Bacteria, fungi, and yeast can be used as chassis organisms for the production of fumaric acid. Genetic modification of microorganisms is considered to be an important tool to increase the yield of fumaric acid. We are able to provide the following services to help our customers achieve the modification of fumaric acid production strains.

Random Mutagenesis

We offer services for UV, radiation, or chemical inducer-driven microbial mutagenesis and help our customers screen for suitable mutant strains.

Homologous and Heterologous Gene Expression

Fumaric acid is produced in the oxidative pathway of the tricarboxylic acid cycle and accumulated in the reductive pathway of the tricarboxylic acid cycle. Multiple enzymes are involved in the synthesis of fumaric acid from pyruvate, and we are able to help our customers overexpress genes encoding related enzymes to increase the production of fumaric acid.

Recombinant Microorganisms

We are able to help our customers construct recombinant microorganisms for fumaric acid production. We are also able to rapidly analyze the metabolic profile of the modified microorganisms using metabolomics analysis techniques.

Optimization of Fermentation Conditions

Fungal Morphology Control

The particulate form of the fungus, which relieves the high viscosity of the fermentation broth and supports the recirculation of subsequent fermentation batches, is considered the most favorable form for fumaric acid production. We are able to control the morphology of the fungi by adjusting the medium composition, trace metal ion concentration, spore concentration, pH, temperature, etc.

Fermentation Parameters Control

We are able to optimize the fermentation conditions for fumaric acid production by controlling fermentation parameters such as temperature, metal ion concentration, and dissolved oxygen concentration to improve the fumaric acid productivity of microorganisms.

Applications of Fumaric Acid

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive fumaric acid production and meet the needs of customers in a variety of industries.

Used as a food acidifier.
Used as an antibacterial agent.
As an intermediate for the preparation of malic acid and aspartic acid.
As a drug delivery system.
Used in the production of synthetic resins and biodegradable polymers.
Participate in the formulation design of drugs for psoriasis treatment.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in fumaric acid production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

References

Chen X, et al. Fumaric acid production by Torulopsis glabrata: engineering the urea cycle and the purine nucleotide cycle. Biotechnol Bioeng. 2015 Jan; 112(1): 156-67.
Xu Q, et al. Key technologies for the industrial production of fumaric acid by fermentation. Biotechnol Adv. 2012 Nov-Dec; 30(6): 1685-96.

Please note that all services are for research use only. Not intended for any clinical use.

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