Strain Engineering for Non-Ribosomal Peptide

CD Biosynsis leverages our specialized synthetic biology platform to help our customers establish an efficient and well-characterized non-ribosomal peptide synthesis system for a wide range of applications in the pharmaceutical, agricultural and industrial sectors. We are committed to helping our customers solve all problems encountered in the production of non-ribosomal peptides.

Advantages of Synthetic Biology-Driven Non-Ribosomal Peptide Production

Non-ribosomal peptides (NRPs) are structurally diverse secondary metabolites synthesized by large enzyme complexes called non-ribosomal peptide synthetases. NRPs have a variety of biological activities and have a wide range of applications in pharmaceutical, agricultural and industrial fields. However, the natural origin of non-ribosomal peptides is extremely limited in quantity and the methods of isolation and extraction are inefficient. In addition, non-ribosomal peptides with complex structures are difficult to produce by conventional chemical synthesis methods. All these issues have led to a significant hindrance in the development of an engineered production platform for non-ribosomal peptides. As an emerging engineering technology, synthetic biology can create an efficient, well-characterized, and sustainable synthetic system for producing non-ribosomal peptides. Our experienced scientists can develop high-quality microbial chassis as green manufacturing factories for the mass production of non-ribosomal peptides.

Figure 1. Synthetic biology tools and physiological traits required for production of non-ribosomal peptides from a heterologous host. (Cook TB, et al., 2019)

What We Provide

CD Biosynsis integrates emerging technologies and advanced tools into our synthetic biology platform to help our customers develop convenient and efficient production methods for non-ribosomal peptides to meet the growing demand for them in the pharmaceutical, agricultural and industrial fields. We are capable of helping our customers to solve all problems encountered in the development of non-ribosomal peptides engineering production platforms.

Deliverables

• Superior non-ribosomal peptide-producing microorganisms.
• High-quality non-ribosomal peptide products.

How We Can Help

Engineering of Microbial Chassis for Non-Ribosomal Peptides Production

We can help our customers select the most suitable microbial strains as hosts (including native hosts or heterologous hosts) from a large number of microorganisms based on their specific production and application needs for non-ribosomal peptides. We can use synthetic biology-based metabolic engineering strategies to directionally modify host microorganisms to obtain desirable traits and to become ideal microbial chassis for the production of non-ribosomal peptides. Our collection of microbial strains used for non-ribosomal peptides production is listed below. If you can't find the strain you are interested in, please fill out the online inquiry form and tell us more about your project.

Synthetic Biology Strategies for Overcoming the Challenges of Non-Ribosomal Peptides Production

We can develop synthetic biology tools and metabolic engineering strategies to construct in vitro, cell-free protein synthesis platforms to help overcome the problems encountered in the traditional production of non-ribosomal peptides. Our scientists can optimize the biosynthetic pathway of non-ribosomal peptides to improve their production yields.

Modification of Non-Ribosomal Peptide Synthase

The core backbone of the non-ribosomal peptide is assembled and synthesized by a modular non-ribosomal peptide synthase. We are able to provide co-adenylation structural domain-specific adjustment, multi-structure exchange, and docking structural domain modification services for different types of non-ribosomal peptide synthase engineering modification strategies.

Applications of Non-Ribosomal Peptides

CD Biosynsis provides customized solutions for synthetic biology-driven production of non-ribosomal peptides to promote their use in a wide range of applications.

The application of non-ribosomal peptides in the pharmaceutical field

• Used in the development of clinical antibiotic drugs.
• Used in the development of clinical antineoplastic drugs.
• Used in the development of clinical immunosuppressive drugs.

Application of non-ribosomal peptides in the agricultural field

• Used in crop protection.

Want to Learn More?

CD Biosynsis provides the most comprehensive and efficient solutions for synthetic biology workflows. We are committed to helping our customers solve all problems encountered in protein and peptide production to advance their applications in a wide range of fields. Each of our deliverables will undergo a rigorous quality inspection test to ensure the reliability and accuracy of the results. If you are interested in our services or have any further questions, please do not hesitate to contact us.

References

1. Cook TB, et al. Leveraging synthetic biology for producing bioactive polyketides and non-ribosomal peptides in bacterial heterologous hosts. Medchemcomm. 2019, 10(5): 668-681.
2. Wu Z, et al. Microbial production of small peptide: pathway engineering and synthetic biology. Microb Biotechnol. 2021, 14(6): 2257-2278.

Please note that all services are for research use only. Not intended for any clinical use.