Protein Expression Service

Overview Capabilities Advantages Services Process FAQs

Overview

Our company specialises in providing top-notch protein expression services. Our services include all steps from gene expression and purification to protein expression and purification, including selection and optimization of multiple expression platforms including bacterial, yeast, insect cell, and mammalian cell platforms. Our experience in the industry is over a decade, we have expressed over 6000 species of recombinant proteins and we can generate 1000 new proteins every year.

Our protein expression solutions are not just focused on efficiency and yield, but also, more importantly, protein function and purity. By optimizing expression and purification processes, we can assure that our customers get only the best recombinant proteins. We also offer in-house technical support to formulate and carry out protein expression and purification protocols as per customers ' requirements.

Our experts can advise you on the most suitable product for mass production and high-throughput screening to help you excel in science and industry. Please feel free to reach out to us for further information on our protein expression offerings.

Capabilities

The following is a breakdown of the protein expression services provided:

Expression system type	Service content	Characteristics
Prokaryote expression system (Escherichia coli)	Gene synthesis and codon optimization, vector construction, expression evaluation, purification	High success rate, suitable for large-scale production, low cost
Eukaryotic expression system (yeast)	Gene synthesis and codon optimization, vector construction, expression evaluation, purification	Economic and post-translational modification
Eukaryotic expression system (insect cells)	Gene synthesis and codon optimization, vector construction, virus packaging and amplification, expression evaluation, purification	Long cycle, suitable for complex enzymes and post-modified proteins
Eukaryotic expression system (mammalian cells)	Gene synthesis and codon optimization, vector construction, transient or stable transfection, expression evaluation, purification	High activity, suitable for biological activity research
Free Cell Expression System	Cloning to expression and purification	Fast and requires no cell culture

Advantages

Our protein expression services can offer customers quick, accurate and high-quality protein products that will enable customers to thrive in science and drug development. Our protein expression services have the following benefits:

Diversified expression platforms

We offer protein expression platforms such as prokaryotic (Escherichia coli), yeast, insect cells (baculovirus), mammalian cells, and so on that are able to address a broad range of protein expression requirements. Each offers advantages: prokaryotic systems, low cost and short culture times, which are all suited for the production of small molecule proteins; mammalian cell systems, on the other hand, are able to post-translate and fold proteins correctly, which is suited for very active and natural proteins.

One-stop service

From gene synthesis and vector design to protein expression and purification, we offer complete one-stop services to help customers manage the whole protein expression workflow. This blended service approach not only increases the efficiency but also makes it easier for customers to move between multiple providers.

Good success rate and instant delivery

Our protein expression success rate is over 95%, and we are able to provide high quality protein products very quickly. For instance, protein expression in cells of mammals can achieve a success rate as high as 95% and large-scale protein production can be achieved in relatively short time (up to 5 weeks).

Customized services

Our highly customized services can be designed for customers according to their own requirements such as choosing the right expression system, labeling, purification techniques, etc. We also offer additional services including endotoxin purification, label removal, sterile processing and lyophilisation to address a wide range of experimental demands.

Large-scale production capacity

From milligrams to kilograms, we have the protein production capacity that will accommodate the different production sizes. For instance, our fermentation tanks can reach 1000L — so it is easy and consistent to produce large quantities of protein at a huge scale.

Experience and technical support

The company has more than 10 years of experience in protein expression and is still focusing on optimizing and developing its technical platform. The ExpreS2 platform, for instance, has more than 10 years of success in producing proteins that are hard to express, and it utilises genetically engineered cell lines that provide a novel way to boost protein production.

Services Process

From gene synthesis, to protein expression, purification, quality control and shipment, we offer protein expression services to meet customers' needs at the highest quality and efficiency.

Contract signing

Upon the receipt of customer requirements, the company immediately communicates with the customer about the technical plan, selects the technical service plan and submits the quotation, communicates with the customer about the price and services, and finally signs the contract.

Project implementation

Gene synthesis and vector construction: Based on the sequence data you have received from the customer, Gene synthesis and codon optimization (optional), Cloning the target fragment into the appropriate expression vector and Sequencing verification.
Expression conditions optimisation: Choose the expression system you want (prokaryotic, yeast, insect cell or mammalian cell) and optimize the expression conditions based on the protein's composition.
Cell transfection and cloning: Expression vector is introduced into host cells and clones with high expression are selected.

Expression and purification

Small-scale expression and culture optimization: Run small-scale expression experiments to detect expression and optimise culture conditions.
Extraction on a large scale and separation: Extraction on a large scale under the best possible conditions is performed and then purified using any kind of chromatographic process (such as affinity chromatography, ion exchange chromatography, hydrophobic chromatography, and gel filtration).
Remove any unwanted tags: (If desired, use protease to eliminate the unwanted purification tags and purify again to get the target protein you want.

Quality control and delivery

Analyses: The quality of the protein is confirmed through SDS-PAGE, UV, HPLC-SEC purity test, etc., and endotoxin analysis (if required).
Proposal report: Create the project report or component analysis report, along with all the details and outputs of the expression and purification process.
Sample delivery: Break samples into warehouses as per client's needs, process the product delivery and delivery report, and generate the invoices to customers.

Contract signing

Project implementation

Expression and purification

Quality control and delivery

FAQs

Here is a breakdown of some frequently asked questions (FAQs) regarding protein expression services:

Q: What is a protein expression service?

A: Protein expression services involves the synthesis of specific proteins in host cells through genetic recombination. These include gene synthesis, vector creation, protein expression, and purification.

Q: What are protein expression systems?

A: The most commonly used expression systems for proteins are: prokaryotic expression systems (eg, Escherichia coli), eukaryotic expression systems (mammalian cells, yeast), baculovirus-insect cell systems, and cell-free expression systems.

Q: What are the benefits of protein expression services?

A: What is special about protein expression services is that they're able to generate massive amounts of high-quality recombinant proteins very quickly and efficiently for scientific, pharmaceutical and biotechnical purposes.

Q: How do Protein expression services work?

A: Protein expression services generally involve gene synthesis, vector design, protein expression, purification and validation. Depending on customer requirements, the process can be slightly different.

Q: What is the optimal protein expression system?

A: Choosing a suitable protein expression system, however, involves determining the target protein's properties (molecular weight, solubility, post-translational modification, etc. ), scale of production and profitability.

Q: What are the costs and periodicities of protein expression services?

A: Protein expression prices and cycle times vary depending on project scope and difficulty. In general, it costs anywhere between few hundred dollars to tens of thousands of dollars for small-scale or massive production, and is usually on weeks or months.

Q: Where do the quality controls for protein expression services go?

A: Protein expression services also provide SDS-PAGE, Western blot, HPLC-SEC purity measurement, and endotoxin testing to verify purity and function of the final product.

Q: What about more frequent problems in protein expression?

A: These problems typically include protein insolubility, low expression, post-translational changes, etc. These issues can be overcome through optimizing culture conditions, modifying expression mechanisms or other purification methods.

Q: Where are the uses of protein expression services?

A: Protein expression services are widely employed in biopharma, fundamental research, diagnostic reagent fabrication, vaccine manufacturing, and more.

Please note that all services are for research use only. Not intended for any clinical use.

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